Limits...
Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates.

Shin YC, Lee JH, Kim MJ, Park JH, Kim SE, Kim JS, Oh JW, Han DW - J Funct Biomater (2015)

Bottom Line: RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry.Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM.These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea. choel15@naver.com.

ABSTRACT
In biomedical applications, there is a need for tissue engineering scaffolds to promote and control cellular behaviors, including adhesion, proliferation and differentiation. In particular, the initial adhesion of cells has a great influence on those cellular behaviors. In this study, we concentrate on developing cell-adhesive substrates applicable for tissue engineering scaffolds. The hybrid nanofiber sheets were prepared by electrospinning poly(lactic-co-glycolic acid) (PLGA) and M13 phage, which was genetically modified to enhance cell adhesion thru expressing RGD peptides on their surface. The RGD peptide is a specific motif of extracellular matrix (ECM) for integrin receptors of cells. RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry. In addition, the initial adhesion and proliferation of four different types of mammalian cells were determined in order to evaluate the potential of RGD-PLGA nanofiber sheets as cell-adhesive substrates. Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM. Furthermore, the initial adhesion and proliferation of cells were significantly enhanced on RGD-PLGA sheets. These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

No MeSH data available.


Related in: MedlinePlus

Initial adhesion of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and primary human aortic smooth muscle cell (HASMC) on the controls (tissue culture plastics, TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493517&req=5

jfb-06-00367-f004: Initial adhesion of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and primary human aortic smooth muscle cell (HASMC) on the controls (tissue culture plastics, TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05.

Mentions: To evaluate the initial cell adhesion on RGD-PLGA nanofiber sheets, the four types of cells were cultured on the nanofiber sheets. The cells used in the present study were the murine macrophage cell line (RAW 264.7 cell), the murine preosteoblastic cell line (MC3T3-E1 cell), the human osteosarcoma cell line (MG-63 cell) and the primary human aortic smooth muscle cell (HASMC). All cells were seeded on the nanofiber sheets, and their initial adhesions were evaluated by measuring the cell viability at six hours based on the mitochondrial activity. As shown in Figure 4, all cells, regardless of cell types or species, showed the highest initial adhesion on the RGD-PLGA nanofiber sheets, whereas they showed the lowest initial adhesion on the pure PLGA nanofiber sheets. These results can be understood by considering the correlation between the cellular behaviors and the hydrophilic property of the substrate surface [29]. In addition, the decorated RGD peptides also contributed to an increase in the initial adhesion. Previous studies have shown that the RGD peptides are proven to enhance cell adhesion [34,35]. Consequently, the RGD-PLGA nanofiber sheets can enhance cell adhesion due to the improvement in the surface hydrophilicity and the decorated RGD peptides.


Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates.

Shin YC, Lee JH, Kim MJ, Park JH, Kim SE, Kim JS, Oh JW, Han DW - J Funct Biomater (2015)

Initial adhesion of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and primary human aortic smooth muscle cell (HASMC) on the controls (tissue culture plastics, TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493517&req=5

jfb-06-00367-f004: Initial adhesion of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and primary human aortic smooth muscle cell (HASMC) on the controls (tissue culture plastics, TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05.
Mentions: To evaluate the initial cell adhesion on RGD-PLGA nanofiber sheets, the four types of cells were cultured on the nanofiber sheets. The cells used in the present study were the murine macrophage cell line (RAW 264.7 cell), the murine preosteoblastic cell line (MC3T3-E1 cell), the human osteosarcoma cell line (MG-63 cell) and the primary human aortic smooth muscle cell (HASMC). All cells were seeded on the nanofiber sheets, and their initial adhesions were evaluated by measuring the cell viability at six hours based on the mitochondrial activity. As shown in Figure 4, all cells, regardless of cell types or species, showed the highest initial adhesion on the RGD-PLGA nanofiber sheets, whereas they showed the lowest initial adhesion on the pure PLGA nanofiber sheets. These results can be understood by considering the correlation between the cellular behaviors and the hydrophilic property of the substrate surface [29]. In addition, the decorated RGD peptides also contributed to an increase in the initial adhesion. Previous studies have shown that the RGD peptides are proven to enhance cell adhesion [34,35]. Consequently, the RGD-PLGA nanofiber sheets can enhance cell adhesion due to the improvement in the surface hydrophilicity and the decorated RGD peptides.

Bottom Line: RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry.Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM.These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea. choel15@naver.com.

ABSTRACT
In biomedical applications, there is a need for tissue engineering scaffolds to promote and control cellular behaviors, including adhesion, proliferation and differentiation. In particular, the initial adhesion of cells has a great influence on those cellular behaviors. In this study, we concentrate on developing cell-adhesive substrates applicable for tissue engineering scaffolds. The hybrid nanofiber sheets were prepared by electrospinning poly(lactic-co-glycolic acid) (PLGA) and M13 phage, which was genetically modified to enhance cell adhesion thru expressing RGD peptides on their surface. The RGD peptide is a specific motif of extracellular matrix (ECM) for integrin receptors of cells. RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry. In addition, the initial adhesion and proliferation of four different types of mammalian cells were determined in order to evaluate the potential of RGD-PLGA nanofiber sheets as cell-adhesive substrates. Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM. Furthermore, the initial adhesion and proliferation of cells were significantly enhanced on RGD-PLGA sheets. These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

No MeSH data available.


Related in: MedlinePlus