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Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates.

Shin YC, Lee JH, Kim MJ, Park JH, Kim SE, Kim JS, Oh JW, Han DW - J Funct Biomater (2015)

Bottom Line: RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry.Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM.These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea. choel15@naver.com.

ABSTRACT
In biomedical applications, there is a need for tissue engineering scaffolds to promote and control cellular behaviors, including adhesion, proliferation and differentiation. In particular, the initial adhesion of cells has a great influence on those cellular behaviors. In this study, we concentrate on developing cell-adhesive substrates applicable for tissue engineering scaffolds. The hybrid nanofiber sheets were prepared by electrospinning poly(lactic-co-glycolic acid) (PLGA) and M13 phage, which was genetically modified to enhance cell adhesion thru expressing RGD peptides on their surface. The RGD peptide is a specific motif of extracellular matrix (ECM) for integrin receptors of cells. RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry. In addition, the initial adhesion and proliferation of four different types of mammalian cells were determined in order to evaluate the potential of RGD-PLGA nanofiber sheets as cell-adhesive substrates. Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM. Furthermore, the initial adhesion and proliferation of cells were significantly enhanced on RGD-PLGA sheets. These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

No MeSH data available.


Related in: MedlinePlus

(A) Water contact angles of the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets. An asterisk (*) denotes a significant difference between the PLGA nanofiber sheet and other groups, p < 0.05. (B) DSC thermogram of the pure PLGA and RGD-PLGA nanofiber sheets.
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jfb-06-00367-f003: (A) Water contact angles of the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets. An asterisk (*) denotes a significant difference between the PLGA nanofiber sheet and other groups, p < 0.05. (B) DSC thermogram of the pure PLGA and RGD-PLGA nanofiber sheets.

Mentions: Figure 3A shows the water contact angles of the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets. The water contact angles were 132.7° ± 3.2°, 83.2° ± 3.3° and 73.2° ± 2.3° for the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets, respectively. The water contact angles of electrospun nanofiber sheets were significantly (p < 0.05) decreased by blending with RGD-M13 phage or collagen. The improvement in the hydrophilicity of the RGD-PLGA nanofiber sheets is comparable to that of the PLGA/Collagen nanofiber sheets as the positive control. The increased hydrophilic nature of the substrate surface can lead to enhanced cellular behaviors, including cell adhesion, migration, proliferation and differentiation [29]. However, although collagen is a very hydrophilic biomaterial, one of the main disadvantages of collagen-based sheets is its rapid degradation behavior [30,31]. Therefore, RGD-PLGA nanofiber sheets are desirable candidates as cell-adhesive substrates, because they have a suitable architecture and a sufficient hydrophilic surface for cell adhesion. We also evaluated the thermal behaviors of PLGA and RGD-PLGA nanofiber sheets by DSC (Figure 3B). The DSC thermogram showed two endotherm peaks for each nanofiber sheets. The first endotherm peak was observed at approximately 48.1 and 50.1 °C for the pure PLGA and RGD-PLGA nanofiber sheets, respectively. This can be due to the glass transition temperature of PLGA ranging between approximately 45–55 °C [32,33]. The second endotherm peak, the endothermic melting peak, was observed at approximately 359.4 and 332.1 °C for the pure PLGA and RGD-PLGA nanofiber sheets, respectively. These results indicated that the thermal behavior of the RGD-PLGA nanofiber sheets is not substantially affected by RGD-M13 phage addition under cell culture conditions, although their thermal stability was slightly decreased at temperatures higher than 300 °C. Therefore, it is suggested that the RGD-PLGA nanofiber sheets can serve as a favorable microenvironment for cell adhesion and proliferation.


Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates.

Shin YC, Lee JH, Kim MJ, Park JH, Kim SE, Kim JS, Oh JW, Han DW - J Funct Biomater (2015)

(A) Water contact angles of the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets. An asterisk (*) denotes a significant difference between the PLGA nanofiber sheet and other groups, p < 0.05. (B) DSC thermogram of the pure PLGA and RGD-PLGA nanofiber sheets.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493517&req=5

jfb-06-00367-f003: (A) Water contact angles of the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets. An asterisk (*) denotes a significant difference between the PLGA nanofiber sheet and other groups, p < 0.05. (B) DSC thermogram of the pure PLGA and RGD-PLGA nanofiber sheets.
Mentions: Figure 3A shows the water contact angles of the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets. The water contact angles were 132.7° ± 3.2°, 83.2° ± 3.3° and 73.2° ± 2.3° for the pure PLGA, RGD-PLGA and PLGA/collagen nanofiber sheets, respectively. The water contact angles of electrospun nanofiber sheets were significantly (p < 0.05) decreased by blending with RGD-M13 phage or collagen. The improvement in the hydrophilicity of the RGD-PLGA nanofiber sheets is comparable to that of the PLGA/Collagen nanofiber sheets as the positive control. The increased hydrophilic nature of the substrate surface can lead to enhanced cellular behaviors, including cell adhesion, migration, proliferation and differentiation [29]. However, although collagen is a very hydrophilic biomaterial, one of the main disadvantages of collagen-based sheets is its rapid degradation behavior [30,31]. Therefore, RGD-PLGA nanofiber sheets are desirable candidates as cell-adhesive substrates, because they have a suitable architecture and a sufficient hydrophilic surface for cell adhesion. We also evaluated the thermal behaviors of PLGA and RGD-PLGA nanofiber sheets by DSC (Figure 3B). The DSC thermogram showed two endotherm peaks for each nanofiber sheets. The first endotherm peak was observed at approximately 48.1 and 50.1 °C for the pure PLGA and RGD-PLGA nanofiber sheets, respectively. This can be due to the glass transition temperature of PLGA ranging between approximately 45–55 °C [32,33]. The second endotherm peak, the endothermic melting peak, was observed at approximately 359.4 and 332.1 °C for the pure PLGA and RGD-PLGA nanofiber sheets, respectively. These results indicated that the thermal behavior of the RGD-PLGA nanofiber sheets is not substantially affected by RGD-M13 phage addition under cell culture conditions, although their thermal stability was slightly decreased at temperatures higher than 300 °C. Therefore, it is suggested that the RGD-PLGA nanofiber sheets can serve as a favorable microenvironment for cell adhesion and proliferation.

Bottom Line: RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry.Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM.These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea. choel15@naver.com.

ABSTRACT
In biomedical applications, there is a need for tissue engineering scaffolds to promote and control cellular behaviors, including adhesion, proliferation and differentiation. In particular, the initial adhesion of cells has a great influence on those cellular behaviors. In this study, we concentrate on developing cell-adhesive substrates applicable for tissue engineering scaffolds. The hybrid nanofiber sheets were prepared by electrospinning poly(lactic-co-glycolic acid) (PLGA) and M13 phage, which was genetically modified to enhance cell adhesion thru expressing RGD peptides on their surface. The RGD peptide is a specific motif of extracellular matrix (ECM) for integrin receptors of cells. RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry. In addition, the initial adhesion and proliferation of four different types of mammalian cells were determined in order to evaluate the potential of RGD-PLGA nanofiber sheets as cell-adhesive substrates. Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM. Furthermore, the initial adhesion and proliferation of cells were significantly enhanced on RGD-PLGA sheets. These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

No MeSH data available.


Related in: MedlinePlus