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Differential cell adhesion of breast cancer stem cells on biomaterial substrate with nanotopographical cues.

Tan KK, Giam CS, Leow MY, Chan CW, Yim EK - J Funct Biomater (2015)

Bottom Line: Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent.Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7.A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns.

View Article: PubMed Central - PubMed

Affiliation: Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411. mbiktkb@nus.edu.sg.

ABSTRACT
Cancer stem cells are speculated to have the capability of self-renewal and re-establishment of tumor heterogeneity, possibly involved in the potential relapse of cancer. CD44+CD24-/lowESA+ cells have been reported to possess tumorigenic properties, and these biomarkers are thought to be highly expressed in breast cancer stem cells. Cell behavior can be influenced by biomolecular and topographical cues in the natural microenvironment. We hypothesized that different cell populations in breast cancer tissue exhibit different adhesion characteristics on substrates with nanotopography. Adhesion characterizations were performed using human mammary epithelial cells (HMEC), breast cancer cell line MCF7 and primary invasive ductal carcinoma (IDC) cells obtained from patients' samples, on micro- and nano-patterned poly-L-lactic acid (PLLA) films. Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent. Cells showed elongation morphology on gratings. The CD44+CD24-/lowESA+ subpopulation in MCF7 and IDC cells showed preferential adhesion on 350-nm gratings. Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7. A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns. Taken together, the study demonstrated that the cancer stem cell subpopulation could be enriched using different nanopatterns. The enriched population could subsequently aid in the isolation and characterization of cancer stem cells.

No MeSH data available.


Related in: MedlinePlus

(A) Number of MCF7 attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, **P < 0.01, mean ± SD, n = 5); (B) Number of HMEC attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, mean ± SD, n = 5). Percentage of CD44+ (C) MCF7 and (D) HMECs from HMEC-MCF7 co-culture. The graph represents the percentage of CD44+ cells (in the CD44+/CellTracker Red− region for MCF7 or the CellTracker Red+ region for HMEC) in an event count of at least 5000 after the 24-hour culture period on the PLLA films. Co-cultures of HMEC and MCF7 were seeded on PLLA films, and after 24 h of culture time, cells were fixed and stained for CD44.
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jfb-06-00241-f004: (A) Number of MCF7 attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, **P < 0.01, mean ± SD, n = 5); (B) Number of HMEC attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, mean ± SD, n = 5). Percentage of CD44+ (C) MCF7 and (D) HMECs from HMEC-MCF7 co-culture. The graph represents the percentage of CD44+ cells (in the CD44+/CellTracker Red− region for MCF7 or the CellTracker Red+ region for HMEC) in an event count of at least 5000 after the 24-hour culture period on the PLLA films. Co-cultures of HMEC and MCF7 were seeded on PLLA films, and after 24 h of culture time, cells were fixed and stained for CD44.

Mentions: The number of attached cells after a certain time point is a key parameter in determining the adhesion behavior of the cells. Cell adhesion is drastically influenced by the surface topography, thus affecting the number of cells that adhere to the substrate [20,21]. The number of attached cells on each of the PLLA films at the time points of 4 and 24 h is presented in Figure 3A and Figure 4A.


Differential cell adhesion of breast cancer stem cells on biomaterial substrate with nanotopographical cues.

Tan KK, Giam CS, Leow MY, Chan CW, Yim EK - J Funct Biomater (2015)

(A) Number of MCF7 attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, **P < 0.01, mean ± SD, n = 5); (B) Number of HMEC attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, mean ± SD, n = 5). Percentage of CD44+ (C) MCF7 and (D) HMECs from HMEC-MCF7 co-culture. The graph represents the percentage of CD44+ cells (in the CD44+/CellTracker Red− region for MCF7 or the CellTracker Red+ region for HMEC) in an event count of at least 5000 after the 24-hour culture period on the PLLA films. Co-cultures of HMEC and MCF7 were seeded on PLLA films, and after 24 h of culture time, cells were fixed and stained for CD44.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493510&req=5

jfb-06-00241-f004: (A) Number of MCF7 attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, **P < 0.01, mean ± SD, n = 5); (B) Number of HMEC attached 24 h after seeding on PLLA films, seeding density 16,000 cells/cm2 (*P < 0.05, mean ± SD, n = 5). Percentage of CD44+ (C) MCF7 and (D) HMECs from HMEC-MCF7 co-culture. The graph represents the percentage of CD44+ cells (in the CD44+/CellTracker Red− region for MCF7 or the CellTracker Red+ region for HMEC) in an event count of at least 5000 after the 24-hour culture period on the PLLA films. Co-cultures of HMEC and MCF7 were seeded on PLLA films, and after 24 h of culture time, cells were fixed and stained for CD44.
Mentions: The number of attached cells after a certain time point is a key parameter in determining the adhesion behavior of the cells. Cell adhesion is drastically influenced by the surface topography, thus affecting the number of cells that adhere to the substrate [20,21]. The number of attached cells on each of the PLLA films at the time points of 4 and 24 h is presented in Figure 3A and Figure 4A.

Bottom Line: Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent.Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7.A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns.

View Article: PubMed Central - PubMed

Affiliation: Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411. mbiktkb@nus.edu.sg.

ABSTRACT
Cancer stem cells are speculated to have the capability of self-renewal and re-establishment of tumor heterogeneity, possibly involved in the potential relapse of cancer. CD44+CD24-/lowESA+ cells have been reported to possess tumorigenic properties, and these biomarkers are thought to be highly expressed in breast cancer stem cells. Cell behavior can be influenced by biomolecular and topographical cues in the natural microenvironment. We hypothesized that different cell populations in breast cancer tissue exhibit different adhesion characteristics on substrates with nanotopography. Adhesion characterizations were performed using human mammary epithelial cells (HMEC), breast cancer cell line MCF7 and primary invasive ductal carcinoma (IDC) cells obtained from patients' samples, on micro- and nano-patterned poly-L-lactic acid (PLLA) films. Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent. Cells showed elongation morphology on gratings. The CD44+CD24-/lowESA+ subpopulation in MCF7 and IDC cells showed preferential adhesion on 350-nm gratings. Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7. A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns. Taken together, the study demonstrated that the cancer stem cell subpopulation could be enriched using different nanopatterns. The enriched population could subsequently aid in the isolation and characterization of cancer stem cells.

No MeSH data available.


Related in: MedlinePlus