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Differential cell adhesion of breast cancer stem cells on biomaterial substrate with nanotopographical cues.

Tan KK, Giam CS, Leow MY, Chan CW, Yim EK - J Funct Biomater (2015)

Bottom Line: Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent.Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7.A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns.

View Article: PubMed Central - PubMed

Affiliation: Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411. mbiktkb@nus.edu.sg.

ABSTRACT
Cancer stem cells are speculated to have the capability of self-renewal and re-establishment of tumor heterogeneity, possibly involved in the potential relapse of cancer. CD44+CD24-/lowESA+ cells have been reported to possess tumorigenic properties, and these biomarkers are thought to be highly expressed in breast cancer stem cells. Cell behavior can be influenced by biomolecular and topographical cues in the natural microenvironment. We hypothesized that different cell populations in breast cancer tissue exhibit different adhesion characteristics on substrates with nanotopography. Adhesion characterizations were performed using human mammary epithelial cells (HMEC), breast cancer cell line MCF7 and primary invasive ductal carcinoma (IDC) cells obtained from patients' samples, on micro- and nano-patterned poly-L-lactic acid (PLLA) films. Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent. Cells showed elongation morphology on gratings. The CD44+CD24-/lowESA+ subpopulation in MCF7 and IDC cells showed preferential adhesion on 350-nm gratings. Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7. A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns. Taken together, the study demonstrated that the cancer stem cell subpopulation could be enriched using different nanopatterns. The enriched population could subsequently aid in the isolation and characterization of cancer stem cells.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence images of (A) MCF7 and (B) human mammary epithelial cells (HMEC) after 24 h of culture. The cells were stained for CD44 (green) and co-stained with either CD24 (red) or ESA (red), with DAPI as the counter-stain of nuclei (blue).
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jfb-06-00241-f002: Immunofluorescence images of (A) MCF7 and (B) human mammary epithelial cells (HMEC) after 24 h of culture. The cells were stained for CD44 (green) and co-stained with either CD24 (red) or ESA (red), with DAPI as the counter-stain of nuclei (blue).

Mentions: Immunofluorescence staining was used to examine the cell morphology and to assess the expression of CD44, CD24 and ESA qualitatively. MCF7 and HMEC have different cell morphologies when seeded on substrates with nanotopography after 24 h of culture (Figure 2). Both cell types attached well and in clusters onto the patterned PLLA films without pre-coating with extracellular matrix protein. While a round morphology was most commonly observed, MCF7 and HMEC were well spread on patterned PLLA compared to unpatterned PLLA (Supplementary Figure S1), and cells were slightly more spread on 1-µm wells, 250-nm wells and 1-µm gratings, although a significant difference was not observed among patterns. Cell elongation was observed on the grating patterns, showing that topography was interacting with cells and might affect differential adhesion within heterogeneous cell populations.


Differential cell adhesion of breast cancer stem cells on biomaterial substrate with nanotopographical cues.

Tan KK, Giam CS, Leow MY, Chan CW, Yim EK - J Funct Biomater (2015)

Immunofluorescence images of (A) MCF7 and (B) human mammary epithelial cells (HMEC) after 24 h of culture. The cells were stained for CD44 (green) and co-stained with either CD24 (red) or ESA (red), with DAPI as the counter-stain of nuclei (blue).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493510&req=5

jfb-06-00241-f002: Immunofluorescence images of (A) MCF7 and (B) human mammary epithelial cells (HMEC) after 24 h of culture. The cells were stained for CD44 (green) and co-stained with either CD24 (red) or ESA (red), with DAPI as the counter-stain of nuclei (blue).
Mentions: Immunofluorescence staining was used to examine the cell morphology and to assess the expression of CD44, CD24 and ESA qualitatively. MCF7 and HMEC have different cell morphologies when seeded on substrates with nanotopography after 24 h of culture (Figure 2). Both cell types attached well and in clusters onto the patterned PLLA films without pre-coating with extracellular matrix protein. While a round morphology was most commonly observed, MCF7 and HMEC were well spread on patterned PLLA compared to unpatterned PLLA (Supplementary Figure S1), and cells were slightly more spread on 1-µm wells, 250-nm wells and 1-µm gratings, although a significant difference was not observed among patterns. Cell elongation was observed on the grating patterns, showing that topography was interacting with cells and might affect differential adhesion within heterogeneous cell populations.

Bottom Line: Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent.Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7.A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns.

View Article: PubMed Central - PubMed

Affiliation: Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411. mbiktkb@nus.edu.sg.

ABSTRACT
Cancer stem cells are speculated to have the capability of self-renewal and re-establishment of tumor heterogeneity, possibly involved in the potential relapse of cancer. CD44+CD24-/lowESA+ cells have been reported to possess tumorigenic properties, and these biomarkers are thought to be highly expressed in breast cancer stem cells. Cell behavior can be influenced by biomolecular and topographical cues in the natural microenvironment. We hypothesized that different cell populations in breast cancer tissue exhibit different adhesion characteristics on substrates with nanotopography. Adhesion characterizations were performed using human mammary epithelial cells (HMEC), breast cancer cell line MCF7 and primary invasive ductal carcinoma (IDC) cells obtained from patients' samples, on micro- and nano-patterned poly-L-lactic acid (PLLA) films. Topography demonstrated a significant effect on cell adhesion, and the effect was cell type dependent. Cells showed elongation morphology on gratings. The CD44+CD24-/lowESA+ subpopulation in MCF7 and IDC cells showed preferential adhesion on 350-nm gratings. Flow cytometry analysis showed that 350-nm gratings captured a significantly higher percentage of CD44+CD24- in MCF7. A slightly higher percentage of CD44+CD24-/lowESA+ was captured on the 350-nm gratings, although no significant difference was observed in the CD44+CD24-ESA+ in IDC cells across patterns. Taken together, the study demonstrated that the cancer stem cell subpopulation could be enriched using different nanopatterns. The enriched population could subsequently aid in the isolation and characterization of cancer stem cells.

No MeSH data available.


Related in: MedlinePlus