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Development of thermosensitive hydrogels of chitosan, sodium and magnesium glycerophosphate for bone regeneration applications.

Lisková J, Bačaková L, Skwarczyńska AL, Musial O, Bliznuk V, De Schamphelaere K, Modrzejewska Z, Douglas TE - J Funct Biomater (2015)

Bottom Line: Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability.Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications.However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic. janefox@biolog.cz.

ABSTRACT
Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

No MeSH data available.


Related in: MedlinePlus

Cytocompatibility and adhesion of MG63 cells. (a) Mitochondrial activity of cells cultured in dilutions of eluates of chitosan/Na-β-GP (Ch Na) and chitosan/Na-β-GP/Mg-GP (Ch Na/Mg) hydrogels. Mean values (n = 3) error bars show standard deviation; (b) Live-Dead staining of cells cultured directly on hydrogels after 2 and 4 days.
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jfb-06-00192-f005: Cytocompatibility and adhesion of MG63 cells. (a) Mitochondrial activity of cells cultured in dilutions of eluates of chitosan/Na-β-GP (Ch Na) and chitosan/Na-β-GP/Mg-GP (Ch Na/Mg) hydrogels. Mean values (n = 3) error bars show standard deviation; (b) Live-Dead staining of cells cultured directly on hydrogels after 2 and 4 days.

Mentions: Experiments with osteoblast-like cells (Figure 5) revealed that neither hydrogel type exhibited cytotoxicity when eluated in the medium. The eluates even slightly supported cell growth (Figure 5a). After 2 days, viable cells were observed on both hydrogel types (Figure 5b). After 4 days, both viable and dead cells were observed on both hydrogel types. In another study, human adipose-derived stem cells were encapsulated in chitosan/Na-β-GP hydrogels of similar composition to those used in this study [30]. After 7 days, both live and dead cells were observed.


Development of thermosensitive hydrogels of chitosan, sodium and magnesium glycerophosphate for bone regeneration applications.

Lisková J, Bačaková L, Skwarczyńska AL, Musial O, Bliznuk V, De Schamphelaere K, Modrzejewska Z, Douglas TE - J Funct Biomater (2015)

Cytocompatibility and adhesion of MG63 cells. (a) Mitochondrial activity of cells cultured in dilutions of eluates of chitosan/Na-β-GP (Ch Na) and chitosan/Na-β-GP/Mg-GP (Ch Na/Mg) hydrogels. Mean values (n = 3) error bars show standard deviation; (b) Live-Dead staining of cells cultured directly on hydrogels after 2 and 4 days.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493507&req=5

jfb-06-00192-f005: Cytocompatibility and adhesion of MG63 cells. (a) Mitochondrial activity of cells cultured in dilutions of eluates of chitosan/Na-β-GP (Ch Na) and chitosan/Na-β-GP/Mg-GP (Ch Na/Mg) hydrogels. Mean values (n = 3) error bars show standard deviation; (b) Live-Dead staining of cells cultured directly on hydrogels after 2 and 4 days.
Mentions: Experiments with osteoblast-like cells (Figure 5) revealed that neither hydrogel type exhibited cytotoxicity when eluated in the medium. The eluates even slightly supported cell growth (Figure 5a). After 2 days, viable cells were observed on both hydrogel types (Figure 5b). After 4 days, both viable and dead cells were observed on both hydrogel types. In another study, human adipose-derived stem cells were encapsulated in chitosan/Na-β-GP hydrogels of similar composition to those used in this study [30]. After 7 days, both live and dead cells were observed.

Bottom Line: Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability.Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications.However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic. janefox@biolog.cz.

ABSTRACT
Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

No MeSH data available.


Related in: MedlinePlus