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Development of thermosensitive hydrogels of chitosan, sodium and magnesium glycerophosphate for bone regeneration applications.

Lisková J, Bačaková L, Skwarczyńska AL, Musial O, Bliznuk V, De Schamphelaere K, Modrzejewska Z, Douglas TE - J Funct Biomater (2015)

Bottom Line: Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability.Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications.However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic. janefox@biolog.cz.

ABSTRACT
Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

No MeSH data available.


Related in: MedlinePlus

FTIR spectra of lyophilized of chitosan/Na-β-GP (a) and chitosan/Na-β-GP/Mg-GP (b) hydrogels containing 2.5 mg/mL ALP after incubation for 14 days in a simulated body fluid (SBF).
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jfb-06-00192-f003: FTIR spectra of lyophilized of chitosan/Na-β-GP (a) and chitosan/Na-β-GP/Mg-GP (b) hydrogels containing 2.5 mg/mL ALP after incubation for 14 days in a simulated body fluid (SBF).

Mentions: ATR-FTIR analysis (Figure 3) demonstrated that the mineral formed in both hydrogel types was apatite. Bands characteristic for phosphate υ4 and υ3 stretching were seen in the regions 600–500 cm−1 and 1200–800 cm−1, respectively [22]. Both hydrogel types showed a sharp and very intensive band at approximately 1020 cm−1 and a double band at approximately 540 and 600 cm−1. These are typical for apatite [22,23].


Development of thermosensitive hydrogels of chitosan, sodium and magnesium glycerophosphate for bone regeneration applications.

Lisková J, Bačaková L, Skwarczyńska AL, Musial O, Bliznuk V, De Schamphelaere K, Modrzejewska Z, Douglas TE - J Funct Biomater (2015)

FTIR spectra of lyophilized of chitosan/Na-β-GP (a) and chitosan/Na-β-GP/Mg-GP (b) hydrogels containing 2.5 mg/mL ALP after incubation for 14 days in a simulated body fluid (SBF).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493507&req=5

jfb-06-00192-f003: FTIR spectra of lyophilized of chitosan/Na-β-GP (a) and chitosan/Na-β-GP/Mg-GP (b) hydrogels containing 2.5 mg/mL ALP after incubation for 14 days in a simulated body fluid (SBF).
Mentions: ATR-FTIR analysis (Figure 3) demonstrated that the mineral formed in both hydrogel types was apatite. Bands characteristic for phosphate υ4 and υ3 stretching were seen in the regions 600–500 cm−1 and 1200–800 cm−1, respectively [22]. Both hydrogel types showed a sharp and very intensive band at approximately 1020 cm−1 and a double band at approximately 540 and 600 cm−1. These are typical for apatite [22,23].

Bottom Line: Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability.Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications.However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic. janefox@biolog.cz.

ABSTRACT
Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.

No MeSH data available.


Related in: MedlinePlus