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Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts.

Abdalrahman LS, Wells H, Fakhr MK - Pathogens (2015)

Bottom Line: The hemolysin genes, particularly hlb, were more prevalent in isolates from beef livers.Molecular typing of a subset of the recovered isolates showed that they are highly diverse where spa typing was more discriminatory than PFGE.The alarmingly high incidence of S. aureus in retail beef livers in this study should raise awareness about the food safety of such meat products.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA. Lubna-abdalrahman@utulsa.edu.

ABSTRACT
Staphylococcus aureus is one of the top five pathogens contributing to acquired foodborne illnesses causing an estimated quarter million cases every year in the US. The objectives of this study were to determine the prevalence of Methicillin Susceptible S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA) in retail beef livers, beef, and pork meats sold in Tulsa, Oklahoma and to characterize the recovered strains for their virulence and antimicrobial resistance. Ninety six chilled retail beef (50 beef livers and 46 beef other cuts), and 99 pork meat samples were collected. The prevalence in beef livers was 40/50 (80%) followed by other beef cuts 23/46 (50%) then pork 43/99 (43.3%). No isolates were positive for MRSA since none harbored the mecA or mecC gene. A total of 334 recovered S. aureus isolates (143 beef livers, 76 beef, and 115 pork isolates) were screened for their antimicrobial susceptibility against 16 different antimicrobials and their possession of 18 different toxin genes. Multidrug resistance was more prevalent in the pork isolates followed by beef then beef livers. The prevalence of enterotoxin genes such as seg, seh, and sei and the toxic shock syndrome gene tst was higher in the pork isolates than in the beef ones. The hemolysin genes, particularly hlb, were more prevalent in isolates from beef livers. Molecular typing of a subset of the recovered isolates showed that they are highly diverse where spa typing was more discriminatory than PFGE. The alarmingly high incidence of S. aureus in retail beef livers in this study should raise awareness about the food safety of such meat products.

No MeSH data available.


Related in: MedlinePlus

Distribution of the total number of Staphylococcus aureus isolates isolated from beef livers, beef, and pork according to their Multidrug Resistance (MDR) to one to four antimicrobials, five to seven antimicrobials, and more than seven antimicrobials of the 16 antimicrobials tested.
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pathogens-04-00182-f001: Distribution of the total number of Staphylococcus aureus isolates isolated from beef livers, beef, and pork according to their Multidrug Resistance (MDR) to one to four antimicrobials, five to seven antimicrobials, and more than seven antimicrobials of the 16 antimicrobials tested.

Mentions: The distribution of Multidrug Resistance (MDR) among the 219 S. aureus beef isolates (143 beef livers and 76 other beef cuts) was as follows: 128 beef liver isolates and 43 other beef cuts isolates were resistant to one to four antimicrobials, 13 beef liver isolates and 16 other beef cuts isolates were resistant to five to seven antimicrobials, and two beef liver isolates and 17 other beef cuts isolates were resistant to more than seven antimicrobials (Figure 1). Also, the distribution of Multidrug Resistance (MDR) among the 115 S. aureus pork isolates was as follows: 12 isolates resistant to one to four antimicrobials, 31 isolates resistant to five to seven antimicrobials, and 72 isolates resistant to more than seven antimicrobials (Figure 1). Most of the pork isolates were highly multidrug resistant being resistant to more than seven antimicrobials. Also, most beef livers isolates were resistant to one to four antimicrobials indicating a lower level of multidrug resistance than the beef and pork ones.


Staphylococcus aureus is More Prevalent in Retail Beef Livers than in Pork and other Beef Cuts.

Abdalrahman LS, Wells H, Fakhr MK - Pathogens (2015)

Distribution of the total number of Staphylococcus aureus isolates isolated from beef livers, beef, and pork according to their Multidrug Resistance (MDR) to one to four antimicrobials, five to seven antimicrobials, and more than seven antimicrobials of the 16 antimicrobials tested.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493469&req=5

pathogens-04-00182-f001: Distribution of the total number of Staphylococcus aureus isolates isolated from beef livers, beef, and pork according to their Multidrug Resistance (MDR) to one to four antimicrobials, five to seven antimicrobials, and more than seven antimicrobials of the 16 antimicrobials tested.
Mentions: The distribution of Multidrug Resistance (MDR) among the 219 S. aureus beef isolates (143 beef livers and 76 other beef cuts) was as follows: 128 beef liver isolates and 43 other beef cuts isolates were resistant to one to four antimicrobials, 13 beef liver isolates and 16 other beef cuts isolates were resistant to five to seven antimicrobials, and two beef liver isolates and 17 other beef cuts isolates were resistant to more than seven antimicrobials (Figure 1). Also, the distribution of Multidrug Resistance (MDR) among the 115 S. aureus pork isolates was as follows: 12 isolates resistant to one to four antimicrobials, 31 isolates resistant to five to seven antimicrobials, and 72 isolates resistant to more than seven antimicrobials (Figure 1). Most of the pork isolates were highly multidrug resistant being resistant to more than seven antimicrobials. Also, most beef livers isolates were resistant to one to four antimicrobials indicating a lower level of multidrug resistance than the beef and pork ones.

Bottom Line: The hemolysin genes, particularly hlb, were more prevalent in isolates from beef livers.Molecular typing of a subset of the recovered isolates showed that they are highly diverse where spa typing was more discriminatory than PFGE.The alarmingly high incidence of S. aureus in retail beef livers in this study should raise awareness about the food safety of such meat products.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA. Lubna-abdalrahman@utulsa.edu.

ABSTRACT
Staphylococcus aureus is one of the top five pathogens contributing to acquired foodborne illnesses causing an estimated quarter million cases every year in the US. The objectives of this study were to determine the prevalence of Methicillin Susceptible S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA) in retail beef livers, beef, and pork meats sold in Tulsa, Oklahoma and to characterize the recovered strains for their virulence and antimicrobial resistance. Ninety six chilled retail beef (50 beef livers and 46 beef other cuts), and 99 pork meat samples were collected. The prevalence in beef livers was 40/50 (80%) followed by other beef cuts 23/46 (50%) then pork 43/99 (43.3%). No isolates were positive for MRSA since none harbored the mecA or mecC gene. A total of 334 recovered S. aureus isolates (143 beef livers, 76 beef, and 115 pork isolates) were screened for their antimicrobial susceptibility against 16 different antimicrobials and their possession of 18 different toxin genes. Multidrug resistance was more prevalent in the pork isolates followed by beef then beef livers. The prevalence of enterotoxin genes such as seg, seh, and sei and the toxic shock syndrome gene tst was higher in the pork isolates than in the beef ones. The hemolysin genes, particularly hlb, were more prevalent in isolates from beef livers. Molecular typing of a subset of the recovered isolates showed that they are highly diverse where spa typing was more discriminatory than PFGE. The alarmingly high incidence of S. aureus in retail beef livers in this study should raise awareness about the food safety of such meat products.

No MeSH data available.


Related in: MedlinePlus