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Antibiotic-free selection in biotherapeutics: now and forever.

Mignon C, Sodoyer R, Werle B - Pathogens (2015)

Bottom Line: The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations.Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles.The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means.

View Article: PubMed Central - PubMed

Affiliation: Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France. Charlotte.mignon@bioaster.org.

ABSTRACT
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.

No MeSH data available.


Related in: MedlinePlus

Plasmid selection and maintenance by RNA/RNA interaction. (A): An essential gene is under control of a repressor fused to RNAI homologous sequence. RNAI, produced from plasmid ori (ColE1 derived), binds to this homologous RNA, inhibits the repressor translation and restores the essential gene expression. (B): sacB gene is inserted into the genome, fused to RNA-IN sequence. RNA-OUT, encoded by the plasmid, binds to RNA-IN, inhibiting sacB translation, restoring ability of the strain to grow on sucrose containing medium. GoI: Gene of Interest; ori: Origin of replication.
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pathogens-04-00157-f004: Plasmid selection and maintenance by RNA/RNA interaction. (A): An essential gene is under control of a repressor fused to RNAI homologous sequence. RNAI, produced from plasmid ori (ColE1 derived), binds to this homologous RNA, inhibits the repressor translation and restores the essential gene expression. (B): sacB gene is inserted into the genome, fused to RNA-IN sequence. RNA-OUT, encoded by the plasmid, binds to RNA-IN, inhibiting sacB translation, restoring ability of the strain to grow on sucrose containing medium. GoI: Gene of Interest; ori: Origin of replication.


Antibiotic-free selection in biotherapeutics: now and forever.

Mignon C, Sodoyer R, Werle B - Pathogens (2015)

Plasmid selection and maintenance by RNA/RNA interaction. (A): An essential gene is under control of a repressor fused to RNAI homologous sequence. RNAI, produced from plasmid ori (ColE1 derived), binds to this homologous RNA, inhibits the repressor translation and restores the essential gene expression. (B): sacB gene is inserted into the genome, fused to RNA-IN sequence. RNA-OUT, encoded by the plasmid, binds to RNA-IN, inhibiting sacB translation, restoring ability of the strain to grow on sucrose containing medium. GoI: Gene of Interest; ori: Origin of replication.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493468&req=5

pathogens-04-00157-f004: Plasmid selection and maintenance by RNA/RNA interaction. (A): An essential gene is under control of a repressor fused to RNAI homologous sequence. RNAI, produced from plasmid ori (ColE1 derived), binds to this homologous RNA, inhibits the repressor translation and restores the essential gene expression. (B): sacB gene is inserted into the genome, fused to RNA-IN sequence. RNA-OUT, encoded by the plasmid, binds to RNA-IN, inhibiting sacB translation, restoring ability of the strain to grow on sucrose containing medium. GoI: Gene of Interest; ori: Origin of replication.
Bottom Line: The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations.Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles.The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means.

View Article: PubMed Central - PubMed

Affiliation: Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France. Charlotte.mignon@bioaster.org.

ABSTRACT
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.

No MeSH data available.


Related in: MedlinePlus