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Antibiotic-free selection in biotherapeutics: now and forever.

Mignon C, Sodoyer R, Werle B - Pathogens (2015)

Bottom Line: The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations.Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles.The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means.

View Article: PubMed Central - PubMed

Affiliation: Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France. Charlotte.mignon@bioaster.org.

ABSTRACT
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.

No MeSH data available.


Related in: MedlinePlus

Plasmid selection or/and maintenance by post-segregational killing. (A): Both toxin and antitoxin genes are on the plasmid allowing plasmid maintenance. Another marker (i.e., antibiotic resistant gene) is still needed to allow strain selection. (B): As the toxin gene is inserted into bacterial genome and antitoxin is plasmid-born, this system allows both selection and plasmid maintenance in absence of any antibiotic. GoI: Gene of Interest; ori: Origin of replication.
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pathogens-04-00157-f003: Plasmid selection or/and maintenance by post-segregational killing. (A): Both toxin and antitoxin genes are on the plasmid allowing plasmid maintenance. Another marker (i.e., antibiotic resistant gene) is still needed to allow strain selection. (B): As the toxin gene is inserted into bacterial genome and antitoxin is plasmid-born, this system allows both selection and plasmid maintenance in absence of any antibiotic. GoI: Gene of Interest; ori: Origin of replication.


Antibiotic-free selection in biotherapeutics: now and forever.

Mignon C, Sodoyer R, Werle B - Pathogens (2015)

Plasmid selection or/and maintenance by post-segregational killing. (A): Both toxin and antitoxin genes are on the plasmid allowing plasmid maintenance. Another marker (i.e., antibiotic resistant gene) is still needed to allow strain selection. (B): As the toxin gene is inserted into bacterial genome and antitoxin is plasmid-born, this system allows both selection and plasmid maintenance in absence of any antibiotic. GoI: Gene of Interest; ori: Origin of replication.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493468&req=5

pathogens-04-00157-f003: Plasmid selection or/and maintenance by post-segregational killing. (A): Both toxin and antitoxin genes are on the plasmid allowing plasmid maintenance. Another marker (i.e., antibiotic resistant gene) is still needed to allow strain selection. (B): As the toxin gene is inserted into bacterial genome and antitoxin is plasmid-born, this system allows both selection and plasmid maintenance in absence of any antibiotic. GoI: Gene of Interest; ori: Origin of replication.
Bottom Line: The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations.Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles.The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means.

View Article: PubMed Central - PubMed

Affiliation: Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France. Charlotte.mignon@bioaster.org.

ABSTRACT
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.

No MeSH data available.


Related in: MedlinePlus