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Antibiotic-free selection in biotherapeutics: now and forever.

Mignon C, Sodoyer R, Werle B - Pathogens (2015)

Bottom Line: The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations.Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles.The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means.

View Article: PubMed Central - PubMed

Affiliation: Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France. Charlotte.mignon@bioaster.org.

ABSTRACT
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.

No MeSH data available.


Related in: MedlinePlus

Plasmid selection and maintenance by auxotrophy complementation. (A): Strain auxotrophy is due to an essential gene mutation or deletion. The auxotrophy is complemented by the plasmid-born expression of the functional gene. (B): Strain auxotrophy is due to an amber mutation in an essential gene. Expression of the suppressor tRNA correcting amber mutation can complement the auxotrophy. GoI: Gene of Interest; ori: Origin of replication.
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pathogens-04-00157-f001: Plasmid selection and maintenance by auxotrophy complementation. (A): Strain auxotrophy is due to an essential gene mutation or deletion. The auxotrophy is complemented by the plasmid-born expression of the functional gene. (B): Strain auxotrophy is due to an amber mutation in an essential gene. Expression of the suppressor tRNA correcting amber mutation can complement the auxotrophy. GoI: Gene of Interest; ori: Origin of replication.


Antibiotic-free selection in biotherapeutics: now and forever.

Mignon C, Sodoyer R, Werle B - Pathogens (2015)

Plasmid selection and maintenance by auxotrophy complementation. (A): Strain auxotrophy is due to an essential gene mutation or deletion. The auxotrophy is complemented by the plasmid-born expression of the functional gene. (B): Strain auxotrophy is due to an amber mutation in an essential gene. Expression of the suppressor tRNA correcting amber mutation can complement the auxotrophy. GoI: Gene of Interest; ori: Origin of replication.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493468&req=5

pathogens-04-00157-f001: Plasmid selection and maintenance by auxotrophy complementation. (A): Strain auxotrophy is due to an essential gene mutation or deletion. The auxotrophy is complemented by the plasmid-born expression of the functional gene. (B): Strain auxotrophy is due to an amber mutation in an essential gene. Expression of the suppressor tRNA correcting amber mutation can complement the auxotrophy. GoI: Gene of Interest; ori: Origin of replication.
Bottom Line: The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations.Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles.The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means.

View Article: PubMed Central - PubMed

Affiliation: Technology Research Institute Bioaster, 317 avenue Jean-Jaurés, 69007 Lyon, France. Charlotte.mignon@bioaster.org.

ABSTRACT
The continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. Among these biologic agents are DNA based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. The use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergoing a profound metamorphosis with the implementation and diversification of alternative selection means. This short review will present examples of alternatives to antibiotic selection and their context of application to highlight their ineluctable invasion of the bio-therapeutic world.

No MeSH data available.


Related in: MedlinePlus