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Recent advances in elucidating the genetic mechanisms of nephrogenesis using zebrafish.

Cheng CN, Verdun VA, Wingert RA - Cells (2015)

Bottom Line: There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development.The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors.Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Center for Zebrafish Research, and Center for Stem Cells and Regenerative Medicine, University of Notre Dame, Notre Dame, IN 46556, USA. ccheng2@nd.edu.

ABSTRACT
The kidney is comprised of working units known as nephrons, which are epithelial tubules that contain a series of specialized cell types organized into a precise pattern of functionally distinct segment domains. There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development. The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors. Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

No MeSH data available.


Model of mecom functions in nephron segmentation. mecom expression is proposed to promote DL formation and inhibit PST fates during proximodistal segment patterning. These activities act in opposition to RA signaling, which promotes PCT and PST formation and has been speculated to inhibit distal fates. Abbreviations: distal early (DE), distal late (DL), intermediate mesoderm (IM), proximal convoluted tubule (PCT), pronephric duct (PD), proximal straight tubule (PST), retinoic acid (RA). [Figure adapted from [68], through terms of the Creative Commons License of the Authors].
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cells-04-00218-f004: Model of mecom functions in nephron segmentation. mecom expression is proposed to promote DL formation and inhibit PST fates during proximodistal segment patterning. These activities act in opposition to RA signaling, which promotes PCT and PST formation and has been speculated to inhibit distal fates. Abbreviations: distal early (DE), distal late (DL), intermediate mesoderm (IM), proximal convoluted tubule (PCT), pronephric duct (PD), proximal straight tubule (PST), retinoic acid (RA). [Figure adapted from [68], through terms of the Creative Commons License of the Authors].

Mentions: During loss of function experiments, these studies found that nephron segmentation defects were elicited following the knockdown of either mecom or sim1a [45,68]. Specifically, mecom morphants display expanded PST, which led to a corresponding distal shift of the DE, as well as a reduced DL segment [68]. Thus, it is likely that mecom regulates normal segmentation patterning by restricting PST fates and promoting DL cell types [68] (Figure 4).


Recent advances in elucidating the genetic mechanisms of nephrogenesis using zebrafish.

Cheng CN, Verdun VA, Wingert RA - Cells (2015)

Model of mecom functions in nephron segmentation. mecom expression is proposed to promote DL formation and inhibit PST fates during proximodistal segment patterning. These activities act in opposition to RA signaling, which promotes PCT and PST formation and has been speculated to inhibit distal fates. Abbreviations: distal early (DE), distal late (DL), intermediate mesoderm (IM), proximal convoluted tubule (PCT), pronephric duct (PD), proximal straight tubule (PST), retinoic acid (RA). [Figure adapted from [68], through terms of the Creative Commons License of the Authors].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493457&req=5

cells-04-00218-f004: Model of mecom functions in nephron segmentation. mecom expression is proposed to promote DL formation and inhibit PST fates during proximodistal segment patterning. These activities act in opposition to RA signaling, which promotes PCT and PST formation and has been speculated to inhibit distal fates. Abbreviations: distal early (DE), distal late (DL), intermediate mesoderm (IM), proximal convoluted tubule (PCT), pronephric duct (PD), proximal straight tubule (PST), retinoic acid (RA). [Figure adapted from [68], through terms of the Creative Commons License of the Authors].
Mentions: During loss of function experiments, these studies found that nephron segmentation defects were elicited following the knockdown of either mecom or sim1a [45,68]. Specifically, mecom morphants display expanded PST, which led to a corresponding distal shift of the DE, as well as a reduced DL segment [68]. Thus, it is likely that mecom regulates normal segmentation patterning by restricting PST fates and promoting DL cell types [68] (Figure 4).

Bottom Line: There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development.The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors.Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Center for Zebrafish Research, and Center for Stem Cells and Regenerative Medicine, University of Notre Dame, Notre Dame, IN 46556, USA. ccheng2@nd.edu.

ABSTRACT
The kidney is comprised of working units known as nephrons, which are epithelial tubules that contain a series of specialized cell types organized into a precise pattern of functionally distinct segment domains. There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development. The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors. Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

No MeSH data available.