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Recent advances in elucidating the genetic mechanisms of nephrogenesis using zebrafish.

Cheng CN, Verdun VA, Wingert RA - Cells (2015)

Bottom Line: There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development.The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors.Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Center for Zebrafish Research, and Center for Stem Cells and Regenerative Medicine, University of Notre Dame, Notre Dame, IN 46556, USA. ccheng2@nd.edu.

ABSTRACT
The kidney is comprised of working units known as nephrons, which are epithelial tubules that contain a series of specialized cell types organized into a precise pattern of functionally distinct segment domains. There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development. The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors. Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

No MeSH data available.


Related in: MedlinePlus

Tubulogenesis of the zebrafish pronephros. (A) The timing of tubulogenesis is coincident with the stages of somitogenesis and organogenesis of the embryo; (B–B”) At 24 hours post fertilization (hpf), the two nephrons have formed distinct tubule lumens that can be detected by immunofluorescence to detect green fluorescent protein (GFP), acetylated tubulin (light blue), Prkc ι/ξ (red) and nuclei (DAPI, blue); (C, D) The precise onset of tubulogenesis occurs at the 20 ss, indicated by white arrowheads, along the proximal, central, and distal regions of the nephron territory, with progressive enlargement of the luminal space at 22 and 26 ss. Abbreviations: aorta (a), lateral plate mesoderm (lpm) notochord (n), somite stage (ss) cardinal vein (v), [Figure adapted from [43,57], through terms of the Creative Commons License of the Authors].
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cells-04-00218-f002: Tubulogenesis of the zebrafish pronephros. (A) The timing of tubulogenesis is coincident with the stages of somitogenesis and organogenesis of the embryo; (B–B”) At 24 hours post fertilization (hpf), the two nephrons have formed distinct tubule lumens that can be detected by immunofluorescence to detect green fluorescent protein (GFP), acetylated tubulin (light blue), Prkc ι/ξ (red) and nuclei (DAPI, blue); (C, D) The precise onset of tubulogenesis occurs at the 20 ss, indicated by white arrowheads, along the proximal, central, and distal regions of the nephron territory, with progressive enlargement of the luminal space at 22 and 26 ss. Abbreviations: aorta (a), lateral plate mesoderm (lpm) notochord (n), somite stage (ss) cardinal vein (v), [Figure adapted from [43,57], through terms of the Creative Commons License of the Authors].

Mentions: Recently, the timing of nephron tubulogenesis and polarity establishment in the zebrafish pronephros were ascertained through histological and immunofluorescence studies [57]. The authors demonstrated that renal progenitors undergo a MET to form a lumen at approximately the 20 somite stage (ss) [57] (Figure 2), which coincides with the regional expression of various tight junction components in the pronephros, such as cldn15a and cldn8 [58].


Recent advances in elucidating the genetic mechanisms of nephrogenesis using zebrafish.

Cheng CN, Verdun VA, Wingert RA - Cells (2015)

Tubulogenesis of the zebrafish pronephros. (A) The timing of tubulogenesis is coincident with the stages of somitogenesis and organogenesis of the embryo; (B–B”) At 24 hours post fertilization (hpf), the two nephrons have formed distinct tubule lumens that can be detected by immunofluorescence to detect green fluorescent protein (GFP), acetylated tubulin (light blue), Prkc ι/ξ (red) and nuclei (DAPI, blue); (C, D) The precise onset of tubulogenesis occurs at the 20 ss, indicated by white arrowheads, along the proximal, central, and distal regions of the nephron territory, with progressive enlargement of the luminal space at 22 and 26 ss. Abbreviations: aorta (a), lateral plate mesoderm (lpm) notochord (n), somite stage (ss) cardinal vein (v), [Figure adapted from [43,57], through terms of the Creative Commons License of the Authors].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493457&req=5

cells-04-00218-f002: Tubulogenesis of the zebrafish pronephros. (A) The timing of tubulogenesis is coincident with the stages of somitogenesis and organogenesis of the embryo; (B–B”) At 24 hours post fertilization (hpf), the two nephrons have formed distinct tubule lumens that can be detected by immunofluorescence to detect green fluorescent protein (GFP), acetylated tubulin (light blue), Prkc ι/ξ (red) and nuclei (DAPI, blue); (C, D) The precise onset of tubulogenesis occurs at the 20 ss, indicated by white arrowheads, along the proximal, central, and distal regions of the nephron territory, with progressive enlargement of the luminal space at 22 and 26 ss. Abbreviations: aorta (a), lateral plate mesoderm (lpm) notochord (n), somite stage (ss) cardinal vein (v), [Figure adapted from [43,57], through terms of the Creative Commons License of the Authors].
Mentions: Recently, the timing of nephron tubulogenesis and polarity establishment in the zebrafish pronephros were ascertained through histological and immunofluorescence studies [57]. The authors demonstrated that renal progenitors undergo a MET to form a lumen at approximately the 20 somite stage (ss) [57] (Figure 2), which coincides with the regional expression of various tight junction components in the pronephros, such as cldn15a and cldn8 [58].

Bottom Line: There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development.The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors.Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Center for Zebrafish Research, and Center for Stem Cells and Regenerative Medicine, University of Notre Dame, Notre Dame, IN 46556, USA. ccheng2@nd.edu.

ABSTRACT
The kidney is comprised of working units known as nephrons, which are epithelial tubules that contain a series of specialized cell types organized into a precise pattern of functionally distinct segment domains. There is a limited understanding of the genetic mechanisms that establish these discrete nephron cell types during renal development. The zebrafish embryonic kidney serves as a simplified yet conserved vertebrate model to delineate how nephron segments are patterned from renal progenitors. Here, we provide a concise review of recent advances in this emerging field, and discuss how continued research using zebrafish genetics can be applied to gain insights about nephrogenesis.

No MeSH data available.


Related in: MedlinePlus