c-Myb is required for plasma cell migration to bone marrow after immunization or infection.
Bottom Line: Here, we detail the critical role of the transcription factor c-Myb in determining plasma cell location.This was correlated with a dramatic reduction of plasma cells in peripheral blood, mislocalization in spleen, and an inability of c-Myb-deficient plasma cells to migrate along a CXCL12 gradient.Therefore, c-Myb plays an essential, novel role in establishing the long-lived plasma cell population in the BM via responsiveness to chemokine migration cues.
Affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia firstname.lastname@example.org email@example.com.Show MeSH
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Mentions: To further investigate the ASC defect, c-Mybfl/flCd23Cre/+ mice were crossed with Blimp-1gfp/+ reporter mice (Kallies et al., 2004), within which plasma cell populations can be divided into Blimp-1int and Blimp-1hi populations that have distinct characteristics (Kallies et al., 2004). Blimp-1int plasma cells are short-lived, less mature plasma cells, which is the population that retains migratory potential (Hauser et al., 2002). c-Mybfl/flCd23Cre/+ or c-Mybfl/fl littermates that were Blimp-1gfp/+ were used to track Blimp-1intCD138hi plasmablasts and Blimp-1hiCD138hi plasma cells. Whereas both populations still existed in the BM, mesenteric lymph nodes (MLNs), and spleen, there was a significant increase in Blimp-1int plasmablasts in the absence of c-Myb (Fig. 4, A and B). Furthermore, the majority of BM B220loCD138hi cells were IgM (mean ± SEM: 81 ± 3% versus 34 ± 1.7% in controls), indicating a different route of formation.
Affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia firstname.lastname@example.org email@example.com.