c-Myb is required for plasma cell migration to bone marrow after immunization or infection.
Bottom Line: Here, we detail the critical role of the transcription factor c-Myb in determining plasma cell location.This was correlated with a dramatic reduction of plasma cells in peripheral blood, mislocalization in spleen, and an inability of c-Myb-deficient plasma cells to migrate along a CXCL12 gradient.Therefore, c-Myb plays an essential, novel role in establishing the long-lived plasma cell population in the BM via responsiveness to chemokine migration cues.
Affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia email@example.com firstname.lastname@example.org.Show MeSH
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Mentions: To rule out potential secondary changes in the microenvironment affecting migration of ASCs in c-Mybfl/flCd23Cre/+ mice, mixed BM chimeras were created. Ly5.1 wild-type BM was mixed 1:1 with Ly5.2 c-Mybfl/flCd23Cre/+ (or Cd23Cre/+ only for control chimeras) and used to reconstitute irradiated recipients (Fig. 2 A). Immunization of these chimeras revealed Ly5.1+NP+IgG1+ ASCs were present in the BM (Fig. 2, B and C), as were Cd23Cre/+ Ly5.2+NP+IgG1+ ASCs (Fig. 2 C). In contrast, NP+IgG1+ c-Myb–deficient, BM-resident ASCs were absent (Fig. 2 B). Therefore, although wild-type ASCs migrated to the BM, c-Myb–deficient ASCs did not within the same animal, demonstrating the B cell intrinsic basis of the defect.
Affiliation: Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia email@example.com firstname.lastname@example.org.