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VIGS approach reveals the modulation of anthocyanin biosynthetic genes by CaMYB in chili pepper leaves.

Zhang Z, Li DW, Jin JH, Yin YX, Zhang HX, Chai WG, Gong ZH - Front Plant Sci (2015)

Bottom Line: Silencing of the R2R3-MYB transcription factor CaMYB in pepper leaves of Z1 resulted in the loss of anthocyanin accumulation.The expression of MYC was significantly lower in CaMYB-silenced leaves, whereas WD40 showed the opposite pattern.These results indicated that MYB plays an important role in the regulation of anthocyanin biosynthetic related genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China.

ABSTRACT
The purple coloration of pepper leaves arises from the accumulation of anthocyanin. Three regulatory and 12 structural genes have been characterized for their involvement in the anthocyanin biosynthesis. Examination of the abundance of these genes in leaves showed that the majority of them differed between anthocyanin pigmented line Z1 and non-pigmented line A3. Silencing of the R2R3-MYB transcription factor CaMYB in pepper leaves of Z1 resulted in the loss of anthocyanin accumulation. Moreover, the expression of multiple genes was altered in the silenced leaves. The expression of MYC was significantly lower in CaMYB-silenced leaves, whereas WD40 showed the opposite pattern. Most structural genes including CHS, CHI, F3H, F3'5'H, DFR, ANS, UFGT, ANP, and GST were repressed in CaMYB-silenced foliage with the exception of PAL, C4H, and 4CL. These results indicated that MYB plays an important role in the regulation of anthocyanin biosynthetic related genes. Besides CaMYB silenced leaves rendered more sporulation of Phytophthora capsici Leonian indicating that CaMYB might be involved in the defense response to pathogens.

No MeSH data available.


Disease symptoms developed on the non-infiltrated (CK), empty vector control (pTRV2: 00) and silenced (TRV2: CaMYB) leaves infected by Phytophthora capsici. (A) Non-infiltrated control; (B) leaves were infiltrated with TRV empty vector; (C) leaves were infiltrated with TRV2: CaMYB vector. Pictures were taken at 40 and 48 h after the inoculation.
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Figure 8: Disease symptoms developed on the non-infiltrated (CK), empty vector control (pTRV2: 00) and silenced (TRV2: CaMYB) leaves infected by Phytophthora capsici. (A) Non-infiltrated control; (B) leaves were infiltrated with TRV empty vector; (C) leaves were infiltrated with TRV2: CaMYB vector. Pictures were taken at 40 and 48 h after the inoculation.

Mentions: To determine the role of CaMYB in the basal defense, detached leaves from CK, NC, and pTRV2: CaMYB plants were exposed to an 8 mm diameter mycelium plug from P. capsici 21 days after the performance of the VIGS. Water-soaked lesions occurred 40 h after inoculation on the silenced Z1 leaves, and 8 h later the whole leaves became water-soaked (Figure 8). In contrast, water-soaked lesions were visible on CK and the NC plants at 72 h after inoculation and the symptoms developed slowly.


VIGS approach reveals the modulation of anthocyanin biosynthetic genes by CaMYB in chili pepper leaves.

Zhang Z, Li DW, Jin JH, Yin YX, Zhang HX, Chai WG, Gong ZH - Front Plant Sci (2015)

Disease symptoms developed on the non-infiltrated (CK), empty vector control (pTRV2: 00) and silenced (TRV2: CaMYB) leaves infected by Phytophthora capsici. (A) Non-infiltrated control; (B) leaves were infiltrated with TRV empty vector; (C) leaves were infiltrated with TRV2: CaMYB vector. Pictures were taken at 40 and 48 h after the inoculation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493389&req=5

Figure 8: Disease symptoms developed on the non-infiltrated (CK), empty vector control (pTRV2: 00) and silenced (TRV2: CaMYB) leaves infected by Phytophthora capsici. (A) Non-infiltrated control; (B) leaves were infiltrated with TRV empty vector; (C) leaves were infiltrated with TRV2: CaMYB vector. Pictures were taken at 40 and 48 h after the inoculation.
Mentions: To determine the role of CaMYB in the basal defense, detached leaves from CK, NC, and pTRV2: CaMYB plants were exposed to an 8 mm diameter mycelium plug from P. capsici 21 days after the performance of the VIGS. Water-soaked lesions occurred 40 h after inoculation on the silenced Z1 leaves, and 8 h later the whole leaves became water-soaked (Figure 8). In contrast, water-soaked lesions were visible on CK and the NC plants at 72 h after inoculation and the symptoms developed slowly.

Bottom Line: Silencing of the R2R3-MYB transcription factor CaMYB in pepper leaves of Z1 resulted in the loss of anthocyanin accumulation.The expression of MYC was significantly lower in CaMYB-silenced leaves, whereas WD40 showed the opposite pattern.These results indicated that MYB plays an important role in the regulation of anthocyanin biosynthetic related genes.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University Yangling, China.

ABSTRACT
The purple coloration of pepper leaves arises from the accumulation of anthocyanin. Three regulatory and 12 structural genes have been characterized for their involvement in the anthocyanin biosynthesis. Examination of the abundance of these genes in leaves showed that the majority of them differed between anthocyanin pigmented line Z1 and non-pigmented line A3. Silencing of the R2R3-MYB transcription factor CaMYB in pepper leaves of Z1 resulted in the loss of anthocyanin accumulation. Moreover, the expression of multiple genes was altered in the silenced leaves. The expression of MYC was significantly lower in CaMYB-silenced leaves, whereas WD40 showed the opposite pattern. Most structural genes including CHS, CHI, F3H, F3'5'H, DFR, ANS, UFGT, ANP, and GST were repressed in CaMYB-silenced foliage with the exception of PAL, C4H, and 4CL. These results indicated that MYB plays an important role in the regulation of anthocyanin biosynthetic related genes. Besides CaMYB silenced leaves rendered more sporulation of Phytophthora capsici Leonian indicating that CaMYB might be involved in the defense response to pathogens.

No MeSH data available.