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Redescription of two subterranean amphipods Niphargusmolnari Méhely, 1927 and Niphargusgebhardti Schellenberg, 1934 (Amphipoda, Niphargidae) and their phylogenetic position.

Angyal D, Balázs G, Zakšek V, Krízsik V, Fišer C - Zookeys (2015)

Bottom Line: Using three independent molecular markers we showed that Niphargusgebhardti belongs to the clade distributed between Central and Eastern Europe, whereas phylogenetic relationship of Niphargusmolnari to the rest of Niphargus species is not clear.The two species from the Mecsek Mts. are phylogenetically not closely related.Both species need to be treated as vulnerable according to IUCN Red List of Threatened Species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Zoology, Hungarian Natural History Museum, Baross u. 13, 1088 Budapest, Hungary ; Doctoral School of Animal-and Agricultural Environmental Sciences, Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia, Deák Ferenc u. 16, 8360 Keszthely, Hungary.

ABSTRACT
A detailed redescription of two endemic, cave-dwelling niphargid species of the Hungarian Mecsek Mts., Niphargusmolnari Méhely, 1927 and Niphargusgebhardti Schellenberg, 1934 is given based on newly collected material. Morphology was studied under light microscopy and with scanning electon microscopy. Morphological descriptions are complemented with mitochondrial cytochrome c oxidase subunit I (COI) sequences as barcodes for both species and with notes on their ecology. Using three independent molecular markers we showed that Niphargusgebhardti belongs to the clade distributed between Central and Eastern Europe, whereas phylogenetic relationship of Niphargusmolnari to the rest of Niphargus species is not clear. The two species from the Mecsek Mts. are phylogenetically not closely related. Both species need to be treated as vulnerable according to IUCN Red List of Threatened Species.

No MeSH data available.


Distribution of the two species within the Abaligeti Cave. 1Niphargusmolnari along the stream of the Western 2. collateral 2Niphargusgebhardti in a permanent pool of ’Karthago romjai’ 3Niphargusgebhardti in a permanent pool near the Akácos Sinkhole’s entrance.
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Figure 2: Distribution of the two species within the Abaligeti Cave. 1Niphargusmolnari along the stream of the Western 2. collateral 2Niphargusgebhardti in a permanent pool of ’Karthago romjai’ 3Niphargusgebhardti in a permanent pool near the Akácos Sinkhole’s entrance.

Mentions: Samples for the redescription were collected in the Abaligeti Cave (N46°8'11.89", E18°6'59.40"), which is located in Southern Hungary, Western Mecsek in Abaliget village, near Pécs city. The altitude of the cave entrance is 219 m above sea level. With its three collaterals and the main passage, the total length of the cave is 2000 m. Its lowest point below the entrance is 10 m, while its highest point is 38 m. Shallow pools of water in the cave are of two types: some are formated by dripping water of the dripstones whereas others are filled during floods and contain residual water. The cave was regulary visited between 2010 and 2013 to characterize its fauna. For the morphological and molecular taxonomic analysis in total 18 and 20 specimens of Niphargusmolnari and Niphargusgebhardti respectively were collected on 23 March 2013. Niphargusmolnari was found in the stream of the Western 2. collateral and Niphargusgebhardti was collected from a permanent pool in a lateral chamber of ‘Karthago romjai’ hall in the main passage and from a pool at the end of Western 2. collateral, near Akácos Sinkhole’s entrance (Fig. 2). An additional specimen of Niphargusgebhardti for molecular studies was collected from a pool of the Szajha-felső Sinkhole (46°8'5.4" N, 18°7'8.22 E) 30 m vertical distance and 100 m horizontal distance from the entrance. The cave is situated in the area of a platform right above the Abaligeti Cave, 283 m above sea level. The two caves are supposedly connected, their entrances are approximately 1 km from each other (Dezső 2011). Specimens were collected using entomological (soft) forceps and were fixed and stored in 96% ethanol.


Redescription of two subterranean amphipods Niphargusmolnari Méhely, 1927 and Niphargusgebhardti Schellenberg, 1934 (Amphipoda, Niphargidae) and their phylogenetic position.

Angyal D, Balázs G, Zakšek V, Krízsik V, Fišer C - Zookeys (2015)

Distribution of the two species within the Abaligeti Cave. 1Niphargusmolnari along the stream of the Western 2. collateral 2Niphargusgebhardti in a permanent pool of ’Karthago romjai’ 3Niphargusgebhardti in a permanent pool near the Akácos Sinkhole’s entrance.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493343&req=5

Figure 2: Distribution of the two species within the Abaligeti Cave. 1Niphargusmolnari along the stream of the Western 2. collateral 2Niphargusgebhardti in a permanent pool of ’Karthago romjai’ 3Niphargusgebhardti in a permanent pool near the Akácos Sinkhole’s entrance.
Mentions: Samples for the redescription were collected in the Abaligeti Cave (N46°8'11.89", E18°6'59.40"), which is located in Southern Hungary, Western Mecsek in Abaliget village, near Pécs city. The altitude of the cave entrance is 219 m above sea level. With its three collaterals and the main passage, the total length of the cave is 2000 m. Its lowest point below the entrance is 10 m, while its highest point is 38 m. Shallow pools of water in the cave are of two types: some are formated by dripping water of the dripstones whereas others are filled during floods and contain residual water. The cave was regulary visited between 2010 and 2013 to characterize its fauna. For the morphological and molecular taxonomic analysis in total 18 and 20 specimens of Niphargusmolnari and Niphargusgebhardti respectively were collected on 23 March 2013. Niphargusmolnari was found in the stream of the Western 2. collateral and Niphargusgebhardti was collected from a permanent pool in a lateral chamber of ‘Karthago romjai’ hall in the main passage and from a pool at the end of Western 2. collateral, near Akácos Sinkhole’s entrance (Fig. 2). An additional specimen of Niphargusgebhardti for molecular studies was collected from a pool of the Szajha-felső Sinkhole (46°8'5.4" N, 18°7'8.22 E) 30 m vertical distance and 100 m horizontal distance from the entrance. The cave is situated in the area of a platform right above the Abaligeti Cave, 283 m above sea level. The two caves are supposedly connected, their entrances are approximately 1 km from each other (Dezső 2011). Specimens were collected using entomological (soft) forceps and were fixed and stored in 96% ethanol.

Bottom Line: Using three independent molecular markers we showed that Niphargusgebhardti belongs to the clade distributed between Central and Eastern Europe, whereas phylogenetic relationship of Niphargusmolnari to the rest of Niphargus species is not clear.The two species from the Mecsek Mts. are phylogenetically not closely related.Both species need to be treated as vulnerable according to IUCN Red List of Threatened Species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Zoology, Hungarian Natural History Museum, Baross u. 13, 1088 Budapest, Hungary ; Doctoral School of Animal-and Agricultural Environmental Sciences, Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia, Deák Ferenc u. 16, 8360 Keszthely, Hungary.

ABSTRACT
A detailed redescription of two endemic, cave-dwelling niphargid species of the Hungarian Mecsek Mts., Niphargusmolnari Méhely, 1927 and Niphargusgebhardti Schellenberg, 1934 is given based on newly collected material. Morphology was studied under light microscopy and with scanning electon microscopy. Morphological descriptions are complemented with mitochondrial cytochrome c oxidase subunit I (COI) sequences as barcodes for both species and with notes on their ecology. Using three independent molecular markers we showed that Niphargusgebhardti belongs to the clade distributed between Central and Eastern Europe, whereas phylogenetic relationship of Niphargusmolnari to the rest of Niphargus species is not clear. The two species from the Mecsek Mts. are phylogenetically not closely related. Both species need to be treated as vulnerable according to IUCN Red List of Threatened Species.

No MeSH data available.