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Tissue Pharmacology of Da-Cheng-Qi Decoction in Experimental Acute Pancreatitis in Rats.

Zhao X, Zhang Y, Li J, Wan M, Zhu S, Guo H, Xiang J, Thrower EC, Tang W - Evid Based Complement Alternat Med (2015)

Bottom Line: Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD.The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05).Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Sichuan Provincial Pancreatitis Center, West China Hospital, Sichuan University, Chengdu 610041, China.

ABSTRACT
Objectives. The Chinese herbal medicine Da-Cheng-Qi Decoction (DCQD) can ameliorate the severity of acute pancreatitis (AP). However, the potential pharmacological mechanism remains unclear. This study explored the potential effective components and the pharmacokinetic characteristics of DCQD in target tissue in experimental acute pancreatitis in rats. Methods. Acute pancreatitis-like symptoms were first induced in rats and then they were given different doses of DCQD (6 g/kg, 12 g/kg, and 24 g/kg body weight) orally. Tissue drug concentration, tissue pathological score, and inflammatory mediators in pancreas, intestine, and lung tissues of rats were examined after 24 hours, respectively. Results. Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD. The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05). Conclusions. DCQD could alleviate pancreatic, intestinal, and lung injury by altering levels of inflammatory cytokines in AP rats with tissue distribution of its components.

No MeSH data available.


Related in: MedlinePlus

Distribution of different dose of DCQD in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of drug concentration of DCQD. (a) Drug concentration in pancreas tissues. (b) Drug concentration in intestine tissues. (c) Drug concentration in lung tissues. The results are mean ± SE.
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fig3: Distribution of different dose of DCQD in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of drug concentration of DCQD. (a) Drug concentration in pancreas tissues. (b) Drug concentration in intestine tissues. (c) Drug concentration in lung tissues. The results are mean ± SE.

Mentions: Concentrations of major components of DCQD in pancreatic, lung, and intestinal tissue of rats treated with DCQD were measured using a sensitive HPLC-TMS method. The result showed that the five major components of DCQD that were most abundant in the pancreatic tissue of rats after oral administration are naringenin, naringin, rhein, aloe-emodin, and emodin. The concentration of these components increased concurrently with the oral dose of DCQD other than naringenin (Figure 3(a)). Similar results could be found in intestinal and lung tissues (Figures 3(b) and 3(c)). The highest tissue component level of DCQG was rhein in pancreas, naringenin in intestine, and emodin in lung when given high-dose DCQD, respectively (Figure 3). It also was found that the concentration of the major components of DQCD in intestinal tissue was higher than that in pancreas and lung tissue.


Tissue Pharmacology of Da-Cheng-Qi Decoction in Experimental Acute Pancreatitis in Rats.

Zhao X, Zhang Y, Li J, Wan M, Zhu S, Guo H, Xiang J, Thrower EC, Tang W - Evid Based Complement Alternat Med (2015)

Distribution of different dose of DCQD in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of drug concentration of DCQD. (a) Drug concentration in pancreas tissues. (b) Drug concentration in intestine tissues. (c) Drug concentration in lung tissues. The results are mean ± SE.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493295&req=5

fig3: Distribution of different dose of DCQD in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of drug concentration of DCQD. (a) Drug concentration in pancreas tissues. (b) Drug concentration in intestine tissues. (c) Drug concentration in lung tissues. The results are mean ± SE.
Mentions: Concentrations of major components of DCQD in pancreatic, lung, and intestinal tissue of rats treated with DCQD were measured using a sensitive HPLC-TMS method. The result showed that the five major components of DCQD that were most abundant in the pancreatic tissue of rats after oral administration are naringenin, naringin, rhein, aloe-emodin, and emodin. The concentration of these components increased concurrently with the oral dose of DCQD other than naringenin (Figure 3(a)). Similar results could be found in intestinal and lung tissues (Figures 3(b) and 3(c)). The highest tissue component level of DCQG was rhein in pancreas, naringenin in intestine, and emodin in lung when given high-dose DCQD, respectively (Figure 3). It also was found that the concentration of the major components of DQCD in intestinal tissue was higher than that in pancreas and lung tissue.

Bottom Line: Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD.The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05).Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Sichuan Provincial Pancreatitis Center, West China Hospital, Sichuan University, Chengdu 610041, China.

ABSTRACT
Objectives. The Chinese herbal medicine Da-Cheng-Qi Decoction (DCQD) can ameliorate the severity of acute pancreatitis (AP). However, the potential pharmacological mechanism remains unclear. This study explored the potential effective components and the pharmacokinetic characteristics of DCQD in target tissue in experimental acute pancreatitis in rats. Methods. Acute pancreatitis-like symptoms were first induced in rats and then they were given different doses of DCQD (6 g/kg, 12 g/kg, and 24 g/kg body weight) orally. Tissue drug concentration, tissue pathological score, and inflammatory mediators in pancreas, intestine, and lung tissues of rats were examined after 24 hours, respectively. Results. Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD. The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05). Conclusions. DCQD could alleviate pancreatic, intestinal, and lung injury by altering levels of inflammatory cytokines in AP rats with tissue distribution of its components.

No MeSH data available.


Related in: MedlinePlus