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Tissue Pharmacology of Da-Cheng-Qi Decoction in Experimental Acute Pancreatitis in Rats.

Zhao X, Zhang Y, Li J, Wan M, Zhu S, Guo H, Xiang J, Thrower EC, Tang W - Evid Based Complement Alternat Med (2015)

Bottom Line: Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD.The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05).Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Sichuan Provincial Pancreatitis Center, West China Hospital, Sichuan University, Chengdu 610041, China.

ABSTRACT
Objectives. The Chinese herbal medicine Da-Cheng-Qi Decoction (DCQD) can ameliorate the severity of acute pancreatitis (AP). However, the potential pharmacological mechanism remains unclear. This study explored the potential effective components and the pharmacokinetic characteristics of DCQD in target tissue in experimental acute pancreatitis in rats. Methods. Acute pancreatitis-like symptoms were first induced in rats and then they were given different doses of DCQD (6 g/kg, 12 g/kg, and 24 g/kg body weight) orally. Tissue drug concentration, tissue pathological score, and inflammatory mediators in pancreas, intestine, and lung tissues of rats were examined after 24 hours, respectively. Results. Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD. The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05). Conclusions. DCQD could alleviate pancreatic, intestinal, and lung injury by altering levels of inflammatory cytokines in AP rats with tissue distribution of its components.

No MeSH data available.


Related in: MedlinePlus

Effects of different dose of DCQD on the inflammatory cytokines in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of proinflammatory cytokines (IL-6 and TNF-α) and anti-inflammatory cytokines (IL-4 and IL-10). (a) Inflammatory cytokines in pancreas tissues. (b) Inflammatory cytokines in intestine tissues. (c) Inflammatory cytokines in lung tissues. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.
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fig2: Effects of different dose of DCQD on the inflammatory cytokines in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of proinflammatory cytokines (IL-6 and TNF-α) and anti-inflammatory cytokines (IL-4 and IL-10). (a) Inflammatory cytokines in pancreas tissues. (b) Inflammatory cytokines in intestine tissues. (c) Inflammatory cytokines in lung tissues. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.

Mentions: In the AP model group, there was a significant increase of inflammatory cytokine infiltration of pancreatic tissue at 24 h after sodium taurocholate stimulation compared with the sham operation group, especially the proinflammatory cytokine IL-6. In the DCQD-treated group, proinflammatory cytokine (IL-6 and TNF-α) infiltration was significantly lower, and the anti-inflammatory cytokine levels (IL-4 and IL-10) were significantly higher than that in the AP model group (P < 0.05) (Figure 2(a)). The greatest effect was seen in the HDG (P < 0.01). Similar results were found in intestine and lung tissues of experimental AP (Figures 2(b) and 2(c)).


Tissue Pharmacology of Da-Cheng-Qi Decoction in Experimental Acute Pancreatitis in Rats.

Zhao X, Zhang Y, Li J, Wan M, Zhu S, Guo H, Xiang J, Thrower EC, Tang W - Evid Based Complement Alternat Med (2015)

Effects of different dose of DCQD on the inflammatory cytokines in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of proinflammatory cytokines (IL-6 and TNF-α) and anti-inflammatory cytokines (IL-4 and IL-10). (a) Inflammatory cytokines in pancreas tissues. (b) Inflammatory cytokines in intestine tissues. (c) Inflammatory cytokines in lung tissues. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig2: Effects of different dose of DCQD on the inflammatory cytokines in lung, intestine, and pancreas tissues. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for examination of proinflammatory cytokines (IL-6 and TNF-α) and anti-inflammatory cytokines (IL-4 and IL-10). (a) Inflammatory cytokines in pancreas tissues. (b) Inflammatory cytokines in intestine tissues. (c) Inflammatory cytokines in lung tissues. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.
Mentions: In the AP model group, there was a significant increase of inflammatory cytokine infiltration of pancreatic tissue at 24 h after sodium taurocholate stimulation compared with the sham operation group, especially the proinflammatory cytokine IL-6. In the DCQD-treated group, proinflammatory cytokine (IL-6 and TNF-α) infiltration was significantly lower, and the anti-inflammatory cytokine levels (IL-4 and IL-10) were significantly higher than that in the AP model group (P < 0.05) (Figure 2(a)). The greatest effect was seen in the HDG (P < 0.01). Similar results were found in intestine and lung tissues of experimental AP (Figures 2(b) and 2(c)).

Bottom Line: Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD.The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05).Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Sichuan Provincial Pancreatitis Center, West China Hospital, Sichuan University, Chengdu 610041, China.

ABSTRACT
Objectives. The Chinese herbal medicine Da-Cheng-Qi Decoction (DCQD) can ameliorate the severity of acute pancreatitis (AP). However, the potential pharmacological mechanism remains unclear. This study explored the potential effective components and the pharmacokinetic characteristics of DCQD in target tissue in experimental acute pancreatitis in rats. Methods. Acute pancreatitis-like symptoms were first induced in rats and then they were given different doses of DCQD (6 g/kg, 12 g/kg, and 24 g/kg body weight) orally. Tissue drug concentration, tissue pathological score, and inflammatory mediators in pancreas, intestine, and lung tissues of rats were examined after 24 hours, respectively. Results. Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD. The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05). Conclusions. DCQD could alleviate pancreatic, intestinal, and lung injury by altering levels of inflammatory cytokines in AP rats with tissue distribution of its components.

No MeSH data available.


Related in: MedlinePlus