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Tissue Pharmacology of Da-Cheng-Qi Decoction in Experimental Acute Pancreatitis in Rats.

Zhao X, Zhang Y, Li J, Wan M, Zhu S, Guo H, Xiang J, Thrower EC, Tang W - Evid Based Complement Alternat Med (2015)

Bottom Line: Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD.The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05).Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Sichuan Provincial Pancreatitis Center, West China Hospital, Sichuan University, Chengdu 610041, China.

ABSTRACT
Objectives. The Chinese herbal medicine Da-Cheng-Qi Decoction (DCQD) can ameliorate the severity of acute pancreatitis (AP). However, the potential pharmacological mechanism remains unclear. This study explored the potential effective components and the pharmacokinetic characteristics of DCQD in target tissue in experimental acute pancreatitis in rats. Methods. Acute pancreatitis-like symptoms were first induced in rats and then they were given different doses of DCQD (6 g/kg, 12 g/kg, and 24 g/kg body weight) orally. Tissue drug concentration, tissue pathological score, and inflammatory mediators in pancreas, intestine, and lung tissues of rats were examined after 24 hours, respectively. Results. Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD. The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05). Conclusions. DCQD could alleviate pancreatic, intestinal, and lung injury by altering levels of inflammatory cytokines in AP rats with tissue distribution of its components.

No MeSH data available.


Related in: MedlinePlus

DCQD alleviated acute pancreatitis-associated tissue damage. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for pathological examination by hematoxylin and eosin (HE) staining. (a1) Pathological picture of pancreas (HE, ×400). (a2) Pathological scores of pancreas injury. (b1) Pathological picture of intestine (HE, ×100). (b2) Pathological scores of intestine injury. (c1) Pathological picture of lung (HE, ×400). (c2) Pathological scores of lung injury. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.
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fig1: DCQD alleviated acute pancreatitis-associated tissue damage. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for pathological examination by hematoxylin and eosin (HE) staining. (a1) Pathological picture of pancreas (HE, ×400). (a2) Pathological scores of pancreas injury. (b1) Pathological picture of intestine (HE, ×100). (b2) Pathological scores of intestine injury. (c1) Pathological picture of lung (HE, ×400). (c2) Pathological scores of lung injury. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.

Mentions: In NG, the pancreas exhibited no sign of edema and necrosis. However, the MG showed the features of AP characterized by expansion of interstitial edema, extensive infiltration of inflammatory cells, obvious pancreatic acinar cell vacuolization, necrosis, and hemorrhage. The rats treated with DCQD had a significant reduction of inflammatory cell infiltration, hemorrhaging, necrosis, and interstitial edema compared to MG, the greatest effect being seen in the HDG. DCQD reduced the standard pathological scores of the pancreas affected by experimental AP, and the scores of MDG and HDG were significantly lower than that in the MG at 24 hours (Figures 1(a1) and 1(a2)). Similar results could be found in intestinal and lung tissue of animals with experimental AP (Figures 1(b1), 1(b2), 1(c1), and 1(c2)).


Tissue Pharmacology of Da-Cheng-Qi Decoction in Experimental Acute Pancreatitis in Rats.

Zhao X, Zhang Y, Li J, Wan M, Zhu S, Guo H, Xiang J, Thrower EC, Tang W - Evid Based Complement Alternat Med (2015)

DCQD alleviated acute pancreatitis-associated tissue damage. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for pathological examination by hematoxylin and eosin (HE) staining. (a1) Pathological picture of pancreas (HE, ×400). (a2) Pathological scores of pancreas injury. (b1) Pathological picture of intestine (HE, ×100). (b2) Pathological scores of intestine injury. (c1) Pathological picture of lung (HE, ×400). (c2) Pathological scores of lung injury. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493295&req=5

fig1: DCQD alleviated acute pancreatitis-associated tissue damage. Normal group = NG, model group = MG, low-dose group = LDG, medium-dose group = MDG, high-dose group = HDG. Rats (n = 6 per group) were given different dose of DCQD (6 g/kg in LDG, 12 g/kg in MDG, and 24 g/kg in HDG by body weight) 2 h after operation. After 24 h, the lung, intestine, and pancreas tissues were collected for pathological examination by hematoxylin and eosin (HE) staining. (a1) Pathological picture of pancreas (HE, ×400). (a2) Pathological scores of pancreas injury. (b1) Pathological picture of intestine (HE, ×100). (b2) Pathological scores of intestine injury. (c1) Pathological picture of lung (HE, ×400). (c2) Pathological scores of lung injury. The results are mean ± SE. ∗P < 0.05 and ∗∗P < 0.01 versus AP model group.
Mentions: In NG, the pancreas exhibited no sign of edema and necrosis. However, the MG showed the features of AP characterized by expansion of interstitial edema, extensive infiltration of inflammatory cells, obvious pancreatic acinar cell vacuolization, necrosis, and hemorrhage. The rats treated with DCQD had a significant reduction of inflammatory cell infiltration, hemorrhaging, necrosis, and interstitial edema compared to MG, the greatest effect being seen in the HDG. DCQD reduced the standard pathological scores of the pancreas affected by experimental AP, and the scores of MDG and HDG were significantly lower than that in the MG at 24 hours (Figures 1(a1) and 1(a2)). Similar results could be found in intestinal and lung tissue of animals with experimental AP (Figures 1(b1), 1(b2), 1(c1), and 1(c2)).

Bottom Line: Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD.The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05).Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Sichuan Provincial Pancreatitis Center, West China Hospital, Sichuan University, Chengdu 610041, China.

ABSTRACT
Objectives. The Chinese herbal medicine Da-Cheng-Qi Decoction (DCQD) can ameliorate the severity of acute pancreatitis (AP). However, the potential pharmacological mechanism remains unclear. This study explored the potential effective components and the pharmacokinetic characteristics of DCQD in target tissue in experimental acute pancreatitis in rats. Methods. Acute pancreatitis-like symptoms were first induced in rats and then they were given different doses of DCQD (6 g/kg, 12 g/kg, and 24 g/kg body weight) orally. Tissue drug concentration, tissue pathological score, and inflammatory mediators in pancreas, intestine, and lung tissues of rats were examined after 24 hours, respectively. Results. Major components of DCQD could be found in target tissues and their concentrations increased in conjunction with the intake dose of DCQD. The high-dose compounds showed maximal effect on altering levels of anti-inflammatory (interleukin-4 and interleukin-10) and proinflammatory markers (tumor necrosis factor α and interleukin-6) and ameliorating the pathological damage in target tissues (P < 0.05). Conclusions. DCQD could alleviate pancreatic, intestinal, and lung injury by altering levels of inflammatory cytokines in AP rats with tissue distribution of its components.

No MeSH data available.


Related in: MedlinePlus