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Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.

Hong KL, Sooter LJ - Biomed Res Int (2015)

Bottom Line: Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments.There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories.Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Pharmaceutical Sciences, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 20506, USA.

ABSTRACT
Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments. They can bind to user-defined targets with high affinity and specificity. There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories. A large number of target specific nucleic acids MREs and their applications are currently in the literature. This review first describes the general methodologies used in identifying single-stranded DNA (ssDNA) aptamers. It then summarizes advancements in the identification and biosensing application of ssDNA aptamers specific for bacteria, viruses, their associated molecules, and selected chemical toxins. Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

No MeSH data available.


Related in: MedlinePlus

Illustration of examples of ssDNA MRE based mass sensitive biosensors. (a) A representation of a detectable change in resonance frequency upon target binding to immobilized MRE on quartz crystal microbalance. (b) Representation of a detectable nanometer scale bending upon target binding to immobilized MRE on micromechanical cantilever.
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fig8: Illustration of examples of ssDNA MRE based mass sensitive biosensors. (a) A representation of a detectable change in resonance frequency upon target binding to immobilized MRE on quartz crystal microbalance. (b) Representation of a detectable nanometer scale bending upon target binding to immobilized MRE on micromechanical cantilever.

Mentions: A Quartz crystal microbalance (QCM) is an acoustic wave resonator based on the piezoelectric property of quartz crystal. Nucleic acid MREs can be immobilized on gold-coated quartz. The binding between target and MRE increases the mass on the surface of the crystal and leads to a detectable decrease in the resonance frequency of the crystal (Figure 8) [181]. The detection principle of surface acoustic wave (SAW) based biosensor is similar to QCM. Nucleic acid MREs have been utilized to fabricate a special type of Love-wave sensor that uses shear horizontal waves to enhance the surface sensitivity and achieve ultrasensitive detection of the target [269].


Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.

Hong KL, Sooter LJ - Biomed Res Int (2015)

Illustration of examples of ssDNA MRE based mass sensitive biosensors. (a) A representation of a detectable change in resonance frequency upon target binding to immobilized MRE on quartz crystal microbalance. (b) Representation of a detectable nanometer scale bending upon target binding to immobilized MRE on micromechanical cantilever.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493287&req=5

fig8: Illustration of examples of ssDNA MRE based mass sensitive biosensors. (a) A representation of a detectable change in resonance frequency upon target binding to immobilized MRE on quartz crystal microbalance. (b) Representation of a detectable nanometer scale bending upon target binding to immobilized MRE on micromechanical cantilever.
Mentions: A Quartz crystal microbalance (QCM) is an acoustic wave resonator based on the piezoelectric property of quartz crystal. Nucleic acid MREs can be immobilized on gold-coated quartz. The binding between target and MRE increases the mass on the surface of the crystal and leads to a detectable decrease in the resonance frequency of the crystal (Figure 8) [181]. The detection principle of surface acoustic wave (SAW) based biosensor is similar to QCM. Nucleic acid MREs have been utilized to fabricate a special type of Love-wave sensor that uses shear horizontal waves to enhance the surface sensitivity and achieve ultrasensitive detection of the target [269].

Bottom Line: Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments.There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories.Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Pharmaceutical Sciences, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 20506, USA.

ABSTRACT
Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments. They can bind to user-defined targets with high affinity and specificity. There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories. A large number of target specific nucleic acids MREs and their applications are currently in the literature. This review first describes the general methodologies used in identifying single-stranded DNA (ssDNA) aptamers. It then summarizes advancements in the identification and biosensing application of ssDNA aptamers specific for bacteria, viruses, their associated molecules, and selected chemical toxins. Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

No MeSH data available.


Related in: MedlinePlus