Limits...
Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.

Hong KL, Sooter LJ - Biomed Res Int (2015)

Bottom Line: Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments.There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories.Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Pharmaceutical Sciences, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 20506, USA.

ABSTRACT
Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments. They can bind to user-defined targets with high affinity and specificity. There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories. A large number of target specific nucleic acids MREs and their applications are currently in the literature. This review first describes the general methodologies used in identifying single-stranded DNA (ssDNA) aptamers. It then summarizes advancements in the identification and biosensing application of ssDNA aptamers specific for bacteria, viruses, their associated molecules, and selected chemical toxins. Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

No MeSH data available.


Related in: MedlinePlus

Illustration of ssDNA MRE based surface plasmon resonance biosensors. When targets bind to immobilized MREs, a change in the plasmon resonance and plasmon angle will be detected and translated into a real-time response unit.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493287&req=5

fig7: Illustration of ssDNA MRE based surface plasmon resonance biosensors. When targets bind to immobilized MREs, a change in the plasmon resonance and plasmon angle will be detected and translated into a real-time response unit.

Mentions: Surface plasmon resonance (SPR) sensors measure a change in the refractive index and resonance angle when a mass change occurs upon target binding. MREs are often biotin-tagged and immobilized on streptavidin coated gold chip. When targets in solution pass through the flow cell, the binding between targets and immobilized MREs cause a change in mass on the sensor chip surface and is subsequently translated into a change in the refractive index. This change in resonance is proportional to the amount of target bound to the immobilized MREs and therefore providing a real-time detection of the target in solution (Figure 7) [183]. Commercially available SPR systems are typically large and limited to bench top use. One study reported a portable SPR biosensor based on ssDNA MREs for the detection of H5N1 influenza virus [183].


Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.

Hong KL, Sooter LJ - Biomed Res Int (2015)

Illustration of ssDNA MRE based surface plasmon resonance biosensors. When targets bind to immobilized MREs, a change in the plasmon resonance and plasmon angle will be detected and translated into a real-time response unit.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493287&req=5

fig7: Illustration of ssDNA MRE based surface plasmon resonance biosensors. When targets bind to immobilized MREs, a change in the plasmon resonance and plasmon angle will be detected and translated into a real-time response unit.
Mentions: Surface plasmon resonance (SPR) sensors measure a change in the refractive index and resonance angle when a mass change occurs upon target binding. MREs are often biotin-tagged and immobilized on streptavidin coated gold chip. When targets in solution pass through the flow cell, the binding between targets and immobilized MREs cause a change in mass on the sensor chip surface and is subsequently translated into a change in the refractive index. This change in resonance is proportional to the amount of target bound to the immobilized MREs and therefore providing a real-time detection of the target in solution (Figure 7) [183]. Commercially available SPR systems are typically large and limited to bench top use. One study reported a portable SPR biosensor based on ssDNA MREs for the detection of H5N1 influenza virus [183].

Bottom Line: Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments.There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories.Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Pharmaceutical Sciences, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 20506, USA.

ABSTRACT
Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments. They can bind to user-defined targets with high affinity and specificity. There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories. A large number of target specific nucleic acids MREs and their applications are currently in the literature. This review first describes the general methodologies used in identifying single-stranded DNA (ssDNA) aptamers. It then summarizes advancements in the identification and biosensing application of ssDNA aptamers specific for bacteria, viruses, their associated molecules, and selected chemical toxins. Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

No MeSH data available.


Related in: MedlinePlus