Limits...
Gold Nanoparticles Promote Oxidant-Mediated Activation of NF-κB and 53BP1 Recruitment-Based Adaptive Response in Human Astrocytes.

Mytych J, Lewinska A, Zebrowski J, Wnuk M - Biomed Res Int (2015)

Bottom Line: In contrast, nanogold provoked changes in the astrocyte cell cycle and induced senescence-associated β-galactosidase activity.The robust 53BP1 recruitment resulted in reduced micronuclei production.Thus, nanogold treatment stimulated an adaptive response in a human astrocyte cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.

ABSTRACT
Nanogold-based materials are promising candidate tools for nanobased medicine. Nevertheless, no conclusive information on their cytotoxicity is available. In the present study, we investigated the effects of gold nanoparticles (AuNPs) on human astrocytes in vitro. Nanogold treatment in a wide range of concentrations did not result in cytotoxicity. In contrast, nanogold provoked changes in the astrocyte cell cycle and induced senescence-associated β-galactosidase activity. AuNPs promoted oxidative stress and caused activation of NF-κB pathway. After nanogold treatment, an inverse correlation between the formation of 53BP1 foci and micronuclei generation was observed. The robust 53BP1 recruitment resulted in reduced micronuclei production. Thus, nanogold treatment stimulated an adaptive response in a human astrocyte cell.

No MeSH data available.


The effect of nanogold on micronuclei production (a) and 53BP1 recruitment (b). Human astrocytes were treated with 1.1 × 109–5.5 × 1011 AuNPs/mL for 96 h. (a) The cytokinesis-block micronucleus (CBMN) assay. The bars indicate the SD, n = 3, ∗∗∗P < 0.001, and ∗∗P < 0.01 compared with control (ANOVA and Dunnett's a posteriori test). (b) 53BP1 foci were revealed using 53BP1 immunostaining. Cells with 0, 1, 2, 3, and more than 3 53BP1 foci were scored [%].
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493286&req=5

fig4: The effect of nanogold on micronuclei production (a) and 53BP1 recruitment (b). Human astrocytes were treated with 1.1 × 109–5.5 × 1011 AuNPs/mL for 96 h. (a) The cytokinesis-block micronucleus (CBMN) assay. The bars indicate the SD, n = 3, ∗∗∗P < 0.001, and ∗∗P < 0.01 compared with control (ANOVA and Dunnett's a posteriori test). (b) 53BP1 foci were revealed using 53BP1 immunostaining. Cells with 0, 1, 2, 3, and more than 3 53BP1 foci were scored [%].

Mentions: Nanogold did not stimulate micronuclei production (Figure 4(a)).


Gold Nanoparticles Promote Oxidant-Mediated Activation of NF-κB and 53BP1 Recruitment-Based Adaptive Response in Human Astrocytes.

Mytych J, Lewinska A, Zebrowski J, Wnuk M - Biomed Res Int (2015)

The effect of nanogold on micronuclei production (a) and 53BP1 recruitment (b). Human astrocytes were treated with 1.1 × 109–5.5 × 1011 AuNPs/mL for 96 h. (a) The cytokinesis-block micronucleus (CBMN) assay. The bars indicate the SD, n = 3, ∗∗∗P < 0.001, and ∗∗P < 0.01 compared with control (ANOVA and Dunnett's a posteriori test). (b) 53BP1 foci were revealed using 53BP1 immunostaining. Cells with 0, 1, 2, 3, and more than 3 53BP1 foci were scored [%].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493286&req=5

fig4: The effect of nanogold on micronuclei production (a) and 53BP1 recruitment (b). Human astrocytes were treated with 1.1 × 109–5.5 × 1011 AuNPs/mL for 96 h. (a) The cytokinesis-block micronucleus (CBMN) assay. The bars indicate the SD, n = 3, ∗∗∗P < 0.001, and ∗∗P < 0.01 compared with control (ANOVA and Dunnett's a posteriori test). (b) 53BP1 foci were revealed using 53BP1 immunostaining. Cells with 0, 1, 2, 3, and more than 3 53BP1 foci were scored [%].
Mentions: Nanogold did not stimulate micronuclei production (Figure 4(a)).

Bottom Line: In contrast, nanogold provoked changes in the astrocyte cell cycle and induced senescence-associated β-galactosidase activity.The robust 53BP1 recruitment resulted in reduced micronuclei production.Thus, nanogold treatment stimulated an adaptive response in a human astrocyte cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland.

ABSTRACT
Nanogold-based materials are promising candidate tools for nanobased medicine. Nevertheless, no conclusive information on their cytotoxicity is available. In the present study, we investigated the effects of gold nanoparticles (AuNPs) on human astrocytes in vitro. Nanogold treatment in a wide range of concentrations did not result in cytotoxicity. In contrast, nanogold provoked changes in the astrocyte cell cycle and induced senescence-associated β-galactosidase activity. AuNPs promoted oxidative stress and caused activation of NF-κB pathway. After nanogold treatment, an inverse correlation between the formation of 53BP1 foci and micronuclei generation was observed. The robust 53BP1 recruitment resulted in reduced micronuclei production. Thus, nanogold treatment stimulated an adaptive response in a human astrocyte cell.

No MeSH data available.