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Evaluation of the selenotranscriptome expression in two hepatocellular carcinoma cell lines.

Guariniello S, Di Bernardo G, Colonna G, Cammarota M, Castello G, Costantini S - Anal Cell Pathol (Amst) (2015)

Bottom Line: Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis.In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR).Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università degli Studi di Napoli, 80138 Napoli, Italy.

ABSTRACT
Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. Since the selenium is able to fight the oxidative damage which is one of the major origins of cell damage as well as cancer, we have recently focused our attention on selenoprotein family and their involvement in HCC. In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR). Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes. Additionally, interactomic studies were carried out to evaluate the ability of these down- and upregulated genes to interact between them as well as to identify putative HUB nodes representing the centers of correlation able to exercise a direct control over the coordinated genes.

No MeSH data available.


Related in: MedlinePlus

Network analysis: down- and upregulated genes are evidenced by yellow symbols, HUB nodes by cyan symbols, whereas all other genes by white symbols.
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fig2: Network analysis: down- and upregulated genes are evidenced by yellow symbols, HUB nodes by cyan symbols, whereas all other genes by white symbols.

Mentions: We have used the IPA algorithm to study the correlation between down- and upregulated genes. Figure 2 shows that three downregulated (DIO1, DIO2, and SELO) and eight upregulated (GPX4, SELK, SELT, SELV, SEP15, SELN, SEPW1, and TrxR1) genes are connected in the same network named “amino acid metabolism, protein synthesis, and small molecule biochemistry” that presents some nodes (HUB nodes) that bind to our selenoprotein mRNAs: SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4), SP1 (specificity protein 1), SECISBP2 (sec insertion sequence binding protein), NCOR2 (nuclear receptor corepressor 2), and TBL1X (transducin beta-like protein 1X).


Evaluation of the selenotranscriptome expression in two hepatocellular carcinoma cell lines.

Guariniello S, Di Bernardo G, Colonna G, Cammarota M, Castello G, Costantini S - Anal Cell Pathol (Amst) (2015)

Network analysis: down- and upregulated genes are evidenced by yellow symbols, HUB nodes by cyan symbols, whereas all other genes by white symbols.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493270&req=5

fig2: Network analysis: down- and upregulated genes are evidenced by yellow symbols, HUB nodes by cyan symbols, whereas all other genes by white symbols.
Mentions: We have used the IPA algorithm to study the correlation between down- and upregulated genes. Figure 2 shows that three downregulated (DIO1, DIO2, and SELO) and eight upregulated (GPX4, SELK, SELT, SELV, SEP15, SELN, SEPW1, and TrxR1) genes are connected in the same network named “amino acid metabolism, protein synthesis, and small molecule biochemistry” that presents some nodes (HUB nodes) that bind to our selenoprotein mRNAs: SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4), SP1 (specificity protein 1), SECISBP2 (sec insertion sequence binding protein), NCOR2 (nuclear receptor corepressor 2), and TBL1X (transducin beta-like protein 1X).

Bottom Line: Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis.In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR).Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università degli Studi di Napoli, 80138 Napoli, Italy.

ABSTRACT
Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. Since the selenium is able to fight the oxidative damage which is one of the major origins of cell damage as well as cancer, we have recently focused our attention on selenoprotein family and their involvement in HCC. In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR). Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes. Additionally, interactomic studies were carried out to evaluate the ability of these down- and upregulated genes to interact between them as well as to identify putative HUB nodes representing the centers of correlation able to exercise a direct control over the coordinated genes.

No MeSH data available.


Related in: MedlinePlus