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Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


Related in: MedlinePlus

Sagittal cut of the tongue dorsum of mouse oral candidiasis model.Representative images of mouse tongue dorsum infected with C. albicans and stained with periodic acid—Schiff; X400. (A) Healthy control, showing numerous elongated papillae. (B) Untreated control showing numerous yeast cells and hyphal filaments inhabiting the tongue papillae (C) MB treated group and (D) TB treated group showing mild response to treatment (E) Mice tongue treated with GNP-MB and (F) GNP-TB treated group, showing comparatively better inhibition of hyphal population than control or unconjugated PS treated groups (G) Combination of both GNP-MB and GNP-TB showing marked inhibition on the growth of yeast cells as well as hyphae. The population of C. albicans yeast cells and hyphae penetrating the tongue dorsum is indicated by the arrows.
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pone.0131684.g009: Sagittal cut of the tongue dorsum of mouse oral candidiasis model.Representative images of mouse tongue dorsum infected with C. albicans and stained with periodic acid—Schiff; X400. (A) Healthy control, showing numerous elongated papillae. (B) Untreated control showing numerous yeast cells and hyphal filaments inhabiting the tongue papillae (C) MB treated group and (D) TB treated group showing mild response to treatment (E) Mice tongue treated with GNP-MB and (F) GNP-TB treated group, showing comparatively better inhibition of hyphal population than control or unconjugated PS treated groups (G) Combination of both GNP-MB and GNP-TB showing marked inhibition on the growth of yeast cells as well as hyphae. The population of C. albicans yeast cells and hyphae penetrating the tongue dorsum is indicated by the arrows.

Mentions: GNP-MB and GNP-TB combination was also found to be effective in treating oral candidiasis as enumerated by Fig 9. Numerous yeast cells and hyphal filaments are observed harboring the papillae as well as papillary spaces in the untreated control. Upon treatment with various GNP-PS conjugates, a reduction in yeast cells and hyphal filaments is seen although the best result is obtained for GNP-MB and GNP-TB combination where a marked reduction in fungal population is observed with negligible yeast cells and only few hyphal filaments sparsely scattered in the papillae after treatment. MB and TB when given alone also exhibit reduction in fungal population (yeasts and hyphae) as compared to untreated control but the results are not as good as those observed for the conjugates (Fig 9).


Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Sagittal cut of the tongue dorsum of mouse oral candidiasis model.Representative images of mouse tongue dorsum infected with C. albicans and stained with periodic acid—Schiff; X400. (A) Healthy control, showing numerous elongated papillae. (B) Untreated control showing numerous yeast cells and hyphal filaments inhabiting the tongue papillae (C) MB treated group and (D) TB treated group showing mild response to treatment (E) Mice tongue treated with GNP-MB and (F) GNP-TB treated group, showing comparatively better inhibition of hyphal population than control or unconjugated PS treated groups (G) Combination of both GNP-MB and GNP-TB showing marked inhibition on the growth of yeast cells as well as hyphae. The population of C. albicans yeast cells and hyphae penetrating the tongue dorsum is indicated by the arrows.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493101&req=5

pone.0131684.g009: Sagittal cut of the tongue dorsum of mouse oral candidiasis model.Representative images of mouse tongue dorsum infected with C. albicans and stained with periodic acid—Schiff; X400. (A) Healthy control, showing numerous elongated papillae. (B) Untreated control showing numerous yeast cells and hyphal filaments inhabiting the tongue papillae (C) MB treated group and (D) TB treated group showing mild response to treatment (E) Mice tongue treated with GNP-MB and (F) GNP-TB treated group, showing comparatively better inhibition of hyphal population than control or unconjugated PS treated groups (G) Combination of both GNP-MB and GNP-TB showing marked inhibition on the growth of yeast cells as well as hyphae. The population of C. albicans yeast cells and hyphae penetrating the tongue dorsum is indicated by the arrows.
Mentions: GNP-MB and GNP-TB combination was also found to be effective in treating oral candidiasis as enumerated by Fig 9. Numerous yeast cells and hyphal filaments are observed harboring the papillae as well as papillary spaces in the untreated control. Upon treatment with various GNP-PS conjugates, a reduction in yeast cells and hyphal filaments is seen although the best result is obtained for GNP-MB and GNP-TB combination where a marked reduction in fungal population is observed with negligible yeast cells and only few hyphal filaments sparsely scattered in the papillae after treatment. MB and TB when given alone also exhibit reduction in fungal population (yeasts and hyphae) as compared to untreated control but the results are not as good as those observed for the conjugates (Fig 9).

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


Related in: MedlinePlus