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Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


Related in: MedlinePlus

Photodynamic effect of GNP-MB and GNP-TB combination on C. albicans biofilm and planktonic cells.Effect of GNP-MB and GNP-TB alone and in combination against biofilm development (A) and mature biofilm (B). Growth percentage was analyzed by comparing relative metabolic activity (RMA) obtained through XTT assay taking untreated control as 100%. Photodynamic inactivation of planktonic C. albicans in vitro(C).C. albicans yeast cells incubated with GNP-PS complexes, after being irradiated with respective light source were picked (50 μl) and plated onto YPD agar plates for counting CFU. The data represent the mean of three determinants ± SD (Standard Deviation) and are representative of three different experiments (i.e, the experiment was done in triplicate) with similar observations. Statistically groups were compared with control using ANOVA (Analysis of Variance) with the Holm—Sidak test. P values <0.05 were considered statistically significant. GNP-MB+GNP-TB vs Control, P<0.005.
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pone.0131684.g005: Photodynamic effect of GNP-MB and GNP-TB combination on C. albicans biofilm and planktonic cells.Effect of GNP-MB and GNP-TB alone and in combination against biofilm development (A) and mature biofilm (B). Growth percentage was analyzed by comparing relative metabolic activity (RMA) obtained through XTT assay taking untreated control as 100%. Photodynamic inactivation of planktonic C. albicans in vitro(C).C. albicans yeast cells incubated with GNP-PS complexes, after being irradiated with respective light source were picked (50 μl) and plated onto YPD agar plates for counting CFU. The data represent the mean of three determinants ± SD (Standard Deviation) and are representative of three different experiments (i.e, the experiment was done in triplicate) with similar observations. Statistically groups were compared with control using ANOVA (Analysis of Variance) with the Holm—Sidak test. P values <0.05 were considered statistically significant. GNP-MB+GNP-TB vs Control, P<0.005.

Mentions: The XTT assay was performed quantitatively to assess potential of PDT against developing biofilms and mature biofilms (Fig 5A and 5B respectively). Although considerable inhibition of biofilm growth and depletion of mature biofilm was observed upon exposure with either of the PS conjugates, however, therapy employing both GNP-MB and GNP-TB combination simultaneously produced a marked synergistic inhibition of biofilm development as well as marked depletion of mature biofilm. For GNP-MB and GNP-TB conjugates, approx. 40–50% viable biofilm was observed both in the immature as well as fully developed film but the mixture of the two conjugates rendered a significant inhibition and only 18% and 20% viable biofilm could be quantified for immature and mature biofilm respectively at a conc. of 100 μg/ml for the mixture of conjugates.


Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Photodynamic effect of GNP-MB and GNP-TB combination on C. albicans biofilm and planktonic cells.Effect of GNP-MB and GNP-TB alone and in combination against biofilm development (A) and mature biofilm (B). Growth percentage was analyzed by comparing relative metabolic activity (RMA) obtained through XTT assay taking untreated control as 100%. Photodynamic inactivation of planktonic C. albicans in vitro(C).C. albicans yeast cells incubated with GNP-PS complexes, after being irradiated with respective light source were picked (50 μl) and plated onto YPD agar plates for counting CFU. The data represent the mean of three determinants ± SD (Standard Deviation) and are representative of three different experiments (i.e, the experiment was done in triplicate) with similar observations. Statistically groups were compared with control using ANOVA (Analysis of Variance) with the Holm—Sidak test. P values <0.05 were considered statistically significant. GNP-MB+GNP-TB vs Control, P<0.005.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4493101&req=5

pone.0131684.g005: Photodynamic effect of GNP-MB and GNP-TB combination on C. albicans biofilm and planktonic cells.Effect of GNP-MB and GNP-TB alone and in combination against biofilm development (A) and mature biofilm (B). Growth percentage was analyzed by comparing relative metabolic activity (RMA) obtained through XTT assay taking untreated control as 100%. Photodynamic inactivation of planktonic C. albicans in vitro(C).C. albicans yeast cells incubated with GNP-PS complexes, after being irradiated with respective light source were picked (50 μl) and plated onto YPD agar plates for counting CFU. The data represent the mean of three determinants ± SD (Standard Deviation) and are representative of three different experiments (i.e, the experiment was done in triplicate) with similar observations. Statistically groups were compared with control using ANOVA (Analysis of Variance) with the Holm—Sidak test. P values <0.05 were considered statistically significant. GNP-MB+GNP-TB vs Control, P<0.005.
Mentions: The XTT assay was performed quantitatively to assess potential of PDT against developing biofilms and mature biofilms (Fig 5A and 5B respectively). Although considerable inhibition of biofilm growth and depletion of mature biofilm was observed upon exposure with either of the PS conjugates, however, therapy employing both GNP-MB and GNP-TB combination simultaneously produced a marked synergistic inhibition of biofilm development as well as marked depletion of mature biofilm. For GNP-MB and GNP-TB conjugates, approx. 40–50% viable biofilm was observed both in the immature as well as fully developed film but the mixture of the two conjugates rendered a significant inhibition and only 18% and 20% viable biofilm could be quantified for immature and mature biofilm respectively at a conc. of 100 μg/ml for the mixture of conjugates.

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


Related in: MedlinePlus