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Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


Related in: MedlinePlus

Microscopic images revealing photodynamic inhibition of C. albicans biofilm grown on plastic coverslips.(A) Untreated control image of the C. albicans biofilm (B) Biofilm treated with naked GNPs (C) Biofilm exposed to MB (D) Biofilm after treatment with TB (E) GNP-MB (F) GNP-TB treated biofilms (G) GNP-MB and GNP-TB combination exposed C. albicans biofilm.
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pone.0131684.g003: Microscopic images revealing photodynamic inhibition of C. albicans biofilm grown on plastic coverslips.(A) Untreated control image of the C. albicans biofilm (B) Biofilm treated with naked GNPs (C) Biofilm exposed to MB (D) Biofilm after treatment with TB (E) GNP-MB (F) GNP-TB treated biofilms (G) GNP-MB and GNP-TB combination exposed C. albicans biofilm.

Mentions: Both naked as well as PS conjugated GNPs were evaluated for their potential to inactivate C. albicans biofilms grown on plastic coverslips. Figs 3 and 4 display a marked inactivation of C. albicans biofilm upon its sensitization with either GNP-MB or GNP-TB when compared to control. Interestingly, a more pronounced reduction in the biofilm was observed upon its exposure to mixture of GNP-MB and GNP-TB than for the conjugates taken solitarily (Figs 3G and 4G). Although GNP, MB and TB exhibited slight reduction in biofilm as compared to untreated control but the results are much feeble than observed for the conjugates.


Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Microscopic images revealing photodynamic inhibition of C. albicans biofilm grown on plastic coverslips.(A) Untreated control image of the C. albicans biofilm (B) Biofilm treated with naked GNPs (C) Biofilm exposed to MB (D) Biofilm after treatment with TB (E) GNP-MB (F) GNP-TB treated biofilms (G) GNP-MB and GNP-TB combination exposed C. albicans biofilm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493101&req=5

pone.0131684.g003: Microscopic images revealing photodynamic inhibition of C. albicans biofilm grown on plastic coverslips.(A) Untreated control image of the C. albicans biofilm (B) Biofilm treated with naked GNPs (C) Biofilm exposed to MB (D) Biofilm after treatment with TB (E) GNP-MB (F) GNP-TB treated biofilms (G) GNP-MB and GNP-TB combination exposed C. albicans biofilm.
Mentions: Both naked as well as PS conjugated GNPs were evaluated for their potential to inactivate C. albicans biofilms grown on plastic coverslips. Figs 3 and 4 display a marked inactivation of C. albicans biofilm upon its sensitization with either GNP-MB or GNP-TB when compared to control. Interestingly, a more pronounced reduction in the biofilm was observed upon its exposure to mixture of GNP-MB and GNP-TB than for the conjugates taken solitarily (Figs 3G and 4G). Although GNP, MB and TB exhibited slight reduction in biofilm as compared to untreated control but the results are much feeble than observed for the conjugates.

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


Related in: MedlinePlus