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Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


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Transmission electron microscopic images of GNP, GNP-TB and GNP—MB.(A) Biomimetically synthesized naked spherical colloidal GNPs (B) MB-conjugated GNPs (C) TB-conjugated GNPs. Coating of PSs around GNPs is indicated by black arrows.
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pone.0131684.g002: Transmission electron microscopic images of GNP, GNP-TB and GNP—MB.(A) Biomimetically synthesized naked spherical colloidal GNPs (B) MB-conjugated GNPs (C) TB-conjugated GNPs. Coating of PSs around GNPs is indicated by black arrows.

Mentions: Dispersion of colloidal gold nanoparticles in a basic solution (pH 9.0) helped them acquire negative surface charge. The positively charged photosensitizers were allowed to interact electrostatically with GNPs. The conjugation was ascertained by visible spectroscopy and TEM analysis. Fig 1B depicts the UV-visible absorbance spectra of PS conjugated GNPs normalized to the maximum absorbance intensity (the original or un-normalized absorption spectra are shown in S1 Fig). As exhibited in Fig 1B, GNP-MB and GNP-TB conjugates show an additional small shoulder at 540 nm, a characteristic of GNPs which is absent in either unconjugated MB or TB spectrum. The appearance of this additional peak at 540 nm in conjugates suggests conjugation of MB and TB to the GNPs. Furthermore, TEM images also highlight the conjugation of MB and TB to GNP surface (Fig 2). A layer of MB and TB seems to be coated on the surface of dye conjugated GNPs as shown by the arrow heads.


Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice.

Sherwani MA, Tufail S, Khan AA, Owais M - PLoS ONE (2015)

Transmission electron microscopic images of GNP, GNP-TB and GNP—MB.(A) Biomimetically synthesized naked spherical colloidal GNPs (B) MB-conjugated GNPs (C) TB-conjugated GNPs. Coating of PSs around GNPs is indicated by black arrows.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493101&req=5

pone.0131684.g002: Transmission electron microscopic images of GNP, GNP-TB and GNP—MB.(A) Biomimetically synthesized naked spherical colloidal GNPs (B) MB-conjugated GNPs (C) TB-conjugated GNPs. Coating of PSs around GNPs is indicated by black arrows.
Mentions: Dispersion of colloidal gold nanoparticles in a basic solution (pH 9.0) helped them acquire negative surface charge. The positively charged photosensitizers were allowed to interact electrostatically with GNPs. The conjugation was ascertained by visible spectroscopy and TEM analysis. Fig 1B depicts the UV-visible absorbance spectra of PS conjugated GNPs normalized to the maximum absorbance intensity (the original or un-normalized absorption spectra are shown in S1 Fig). As exhibited in Fig 1B, GNP-MB and GNP-TB conjugates show an additional small shoulder at 540 nm, a characteristic of GNPs which is absent in either unconjugated MB or TB spectrum. The appearance of this additional peak at 540 nm in conjugates suggests conjugation of MB and TB to the GNPs. Furthermore, TEM images also highlight the conjugation of MB and TB to GNP surface (Fig 2). A layer of MB and TB seems to be coated on the surface of dye conjugated GNPs as shown by the arrow heads.

Bottom Line: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms.We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm.The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India.

ABSTRACT

Background: Photodynamic therapy (PDT) has been found to be effective in inhibiting biofilm producing organisms. We investigated the photodynamic effect of gold nanoparticle (GNP) conjugated photosensitizers against Candida albicans biofilm. We also examined the photodynamic efficacy of photosensitizer (PS) conjugated GNPs (GNP-PS) to treat skin and oral C. albicans infection in BALB/c mice.

Methods: The biomimetically synthesized GNPs were conjugated to photosensitizers viz. methylene blue (MB) or toluidine blue O (TB). The conjugation of PSs with GNPs was characterized by spectroscopic and microscopic techniques. The efficacy of gold nanoparticle conjugates against C. albicans biofilm was demonstrated by XTT assay and microscopic studies. The therapeutic efficacy of the combination of the GNP conjugates against cutaneous C. albicans infection was examined in mouse model by enumerating residual fungal burden and histopathological studies.

Results: The GNP-PS conjugate based PDT was found to effectively kill both C. albicans planktonic cells and biofilm populating hyphal forms. The mixture of GNPs conjugated to two different PSs significantly depleted the hyphal C. albicans burden against superficial skin and oral C. albicans infection in mice.

Conclusion: The GNP-PS conjugate combination exhibits synergism in photodynamic inactivation of C. albicans. The GNP conjugate based PDT can be employed effectively in treatment of cutaneous C. albicans infections in model animals. The antibiofilm potential of PDT therapy can also be exploited in depletion of C. albicans on medical appliances such as implants and catheters etc.

No MeSH data available.


Related in: MedlinePlus