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Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy.

Kinney SR, Carlson L, Ser-Dolansky J, Thompson C, Shah S, Gambrah A, Xing W, Schneider SS, Mathias CB - PLoS ONE (2015)

Bottom Line: In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation.Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs.In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical and Administrative Sciences, College of Pharmacy, Western New England University, Springfield, MA 01119, United States of America.

ABSTRACT
IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during allergic responses. We assessed whether curcumin ingestion during oral allergen exposure can modulate the development of food allergy using a murine model of ovalbumin (OVA)-induced intestinal anaphylaxis. Herein, we demonstrate that frequent ingestion of curcumin during oral OVA exposure inhibits the development of mastocytosis and intestinal anaphylaxis in OVA-challenged allergic mice. Intragastric (i.g.) exposure to OVA in sensitized BALB/c mice induced a robust IgE-mediated response accompanied by enhanced OVA-IgE levels, intestinal mastocytosis, elevated serum mMCP-1, and acute diarrhea. In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation. Furthermore, allergic diarrhea, mast cell activation and expansion, and Th2 responses were also suppressed in mice exposed to curcumin during the OVA-challenge phase alone, despite the presence of elevated levels of OVA-IgE, suggesting that curcumin may have a direct suppressive effect on intestinal mast cell activation and reverse food allergy symptoms in allergen-sensitized individuals. This was confirmed by observations that curcumin attenuated the expansion of both adoptively transferred bone marrow-derived mast cells (BMMCs), and inhibited their survival and activation during cell culture. Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs. In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

No MeSH data available.


Related in: MedlinePlus

Curcumin inhibits the expansion of mast cells in vivo and inhibits their proliferation, survival and activation in vitro.(A) CFSE+ BMMCs in the peritoneum of curcumin-gavaged mice. (B-D) BMMCs were cultured in the presence of IL-3 and SCF or DNP-IgE with or without 30 μM curcumin for 6 days. Data are representative of 3 or more independent experiments. (B) Numbers of BMMCs; (C) Percentages of apoptotic BMMCs; (D) and assessment of β-hex activity is shown. * = p<0.05 and ** = p<0.01 by Students t-test. † = p<0.0001 and ‡ = p<0.0005 by ANOVA.
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pone.0132467.g008: Curcumin inhibits the expansion of mast cells in vivo and inhibits their proliferation, survival and activation in vitro.(A) CFSE+ BMMCs in the peritoneum of curcumin-gavaged mice. (B-D) BMMCs were cultured in the presence of IL-3 and SCF or DNP-IgE with or without 30 μM curcumin for 6 days. Data are representative of 3 or more independent experiments. (B) Numbers of BMMCs; (C) Percentages of apoptotic BMMCs; (D) and assessment of β-hex activity is shown. * = p<0.05 and ** = p<0.01 by Students t-test. † = p<0.0001 and ‡ = p<0.0005 by ANOVA.

Mentions: Decreased numbers of mast cells in the intestines of curcumin-treated animals suggests that curcumin blocks the homeostasis of these cells in vivo during food allergy. To assess whether curcumin has a direct effect on mast cell expansion, we injected CFSE-labeled BMMCs into the peritoneum of BALB/c mice, and followed their proliferation and survival for six days during curcumin treatments. One week later, the peritoneal lavage was isolated and the numbers of CFSE+-mast cells were assessed. As expected, the numbers of CFSE+ cells had doubled in the peritoneum of untreated BALB/c mice (Fig 8A). In contrast, a similar expansion of CFSE+ cells was not observed in curcumin-treated mice, and in some animals, the numbers of CFSE+ cells had decreased from the original number that were injected (Fig 8A). These data suggest that curcumin directly inhibits the expansion of mast cells in vivo.


Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy.

Kinney SR, Carlson L, Ser-Dolansky J, Thompson C, Shah S, Gambrah A, Xing W, Schneider SS, Mathias CB - PLoS ONE (2015)

Curcumin inhibits the expansion of mast cells in vivo and inhibits their proliferation, survival and activation in vitro.(A) CFSE+ BMMCs in the peritoneum of curcumin-gavaged mice. (B-D) BMMCs were cultured in the presence of IL-3 and SCF or DNP-IgE with or without 30 μM curcumin for 6 days. Data are representative of 3 or more independent experiments. (B) Numbers of BMMCs; (C) Percentages of apoptotic BMMCs; (D) and assessment of β-hex activity is shown. * = p<0.05 and ** = p<0.01 by Students t-test. † = p<0.0001 and ‡ = p<0.0005 by ANOVA.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4493063&req=5

pone.0132467.g008: Curcumin inhibits the expansion of mast cells in vivo and inhibits their proliferation, survival and activation in vitro.(A) CFSE+ BMMCs in the peritoneum of curcumin-gavaged mice. (B-D) BMMCs were cultured in the presence of IL-3 and SCF or DNP-IgE with or without 30 μM curcumin for 6 days. Data are representative of 3 or more independent experiments. (B) Numbers of BMMCs; (C) Percentages of apoptotic BMMCs; (D) and assessment of β-hex activity is shown. * = p<0.05 and ** = p<0.01 by Students t-test. † = p<0.0001 and ‡ = p<0.0005 by ANOVA.
Mentions: Decreased numbers of mast cells in the intestines of curcumin-treated animals suggests that curcumin blocks the homeostasis of these cells in vivo during food allergy. To assess whether curcumin has a direct effect on mast cell expansion, we injected CFSE-labeled BMMCs into the peritoneum of BALB/c mice, and followed their proliferation and survival for six days during curcumin treatments. One week later, the peritoneal lavage was isolated and the numbers of CFSE+-mast cells were assessed. As expected, the numbers of CFSE+ cells had doubled in the peritoneum of untreated BALB/c mice (Fig 8A). In contrast, a similar expansion of CFSE+ cells was not observed in curcumin-treated mice, and in some animals, the numbers of CFSE+ cells had decreased from the original number that were injected (Fig 8A). These data suggest that curcumin directly inhibits the expansion of mast cells in vivo.

Bottom Line: In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation.Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs.In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical and Administrative Sciences, College of Pharmacy, Western New England University, Springfield, MA 01119, United States of America.

ABSTRACT
IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during allergic responses. We assessed whether curcumin ingestion during oral allergen exposure can modulate the development of food allergy using a murine model of ovalbumin (OVA)-induced intestinal anaphylaxis. Herein, we demonstrate that frequent ingestion of curcumin during oral OVA exposure inhibits the development of mastocytosis and intestinal anaphylaxis in OVA-challenged allergic mice. Intragastric (i.g.) exposure to OVA in sensitized BALB/c mice induced a robust IgE-mediated response accompanied by enhanced OVA-IgE levels, intestinal mastocytosis, elevated serum mMCP-1, and acute diarrhea. In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation. Furthermore, allergic diarrhea, mast cell activation and expansion, and Th2 responses were also suppressed in mice exposed to curcumin during the OVA-challenge phase alone, despite the presence of elevated levels of OVA-IgE, suggesting that curcumin may have a direct suppressive effect on intestinal mast cell activation and reverse food allergy symptoms in allergen-sensitized individuals. This was confirmed by observations that curcumin attenuated the expansion of both adoptively transferred bone marrow-derived mast cells (BMMCs), and inhibited their survival and activation during cell culture. Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs. In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

No MeSH data available.


Related in: MedlinePlus