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Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy.

Kinney SR, Carlson L, Ser-Dolansky J, Thompson C, Shah S, Gambrah A, Xing W, Schneider SS, Mathias CB - PLoS ONE (2015)

Bottom Line: In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation.Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs.In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical and Administrative Sciences, College of Pharmacy, Western New England University, Springfield, MA 01119, United States of America.

ABSTRACT
IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during allergic responses. We assessed whether curcumin ingestion during oral allergen exposure can modulate the development of food allergy using a murine model of ovalbumin (OVA)-induced intestinal anaphylaxis. Herein, we demonstrate that frequent ingestion of curcumin during oral OVA exposure inhibits the development of mastocytosis and intestinal anaphylaxis in OVA-challenged allergic mice. Intragastric (i.g.) exposure to OVA in sensitized BALB/c mice induced a robust IgE-mediated response accompanied by enhanced OVA-IgE levels, intestinal mastocytosis, elevated serum mMCP-1, and acute diarrhea. In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation. Furthermore, allergic diarrhea, mast cell activation and expansion, and Th2 responses were also suppressed in mice exposed to curcumin during the OVA-challenge phase alone, despite the presence of elevated levels of OVA-IgE, suggesting that curcumin may have a direct suppressive effect on intestinal mast cell activation and reverse food allergy symptoms in allergen-sensitized individuals. This was confirmed by observations that curcumin attenuated the expansion of both adoptively transferred bone marrow-derived mast cells (BMMCs), and inhibited their survival and activation during cell culture. Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs. In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

No MeSH data available.


Related in: MedlinePlus

Treatment with curcumin inhibits the expression of intestinal Th2 cytokines in allergic mice.Mice were fed with OVA and curcumin as depicted in Fig 1A. (A-H) Expression of jejunal mRNA for various cytokines is shown. Data are representative of 2 independent experiments. * = p<0.05; ** = p<0.01.
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pone.0132467.g003: Treatment with curcumin inhibits the expression of intestinal Th2 cytokines in allergic mice.Mice were fed with OVA and curcumin as depicted in Fig 1A. (A-H) Expression of jejunal mRNA for various cytokines is shown. Data are representative of 2 independent experiments. * = p<0.05; ** = p<0.01.

Mentions: In order to examine whether curcumin suppresses local Th2 responses in the intestines, the jejunae of experimental animals was examined for the expression of the cytokines IL-4, IL-13, IL-5, IL-9, IL-33, IL-10, IL-17, and IFN-γ. As expected, the expression of the classic Th2 cytokines IL-4, IL-5, IL-13, IL-9, and IL-10 was significantly increased in the jejunum of OVA-challenged BALB/c mice compared with saline-treated controls. (Fig 3A–3D and 3F) In contrast, the expression of these cytokines was reduced in the intestines of OVA-challenged, curcumin-treated animals (Fig 3A–3D and 3F). There were no differences in IL-33 or IFN-γ expression in both groups (Fig 3E and 3H). Interestingly however, the increased expression of Th2 cytokines induced by OVA-challenge was also accompanied by a decrease in the expression of the Th17 cytokine, IL-17, in allergic mice (Fig 3G). In contrast, no change in expression of IL-17 was observed in the intestines of OVA-challenged, curcumin-treated mice as compared to saline-sensitized, curcumin-treated control animals (Fig 3G). Taken together, these data suggest that curcumin modulates the T cell response to oral antigen by skewing it away from a Th2-dominated phenotype.


Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy.

Kinney SR, Carlson L, Ser-Dolansky J, Thompson C, Shah S, Gambrah A, Xing W, Schneider SS, Mathias CB - PLoS ONE (2015)

Treatment with curcumin inhibits the expression of intestinal Th2 cytokines in allergic mice.Mice were fed with OVA and curcumin as depicted in Fig 1A. (A-H) Expression of jejunal mRNA for various cytokines is shown. Data are representative of 2 independent experiments. * = p<0.05; ** = p<0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493063&req=5

pone.0132467.g003: Treatment with curcumin inhibits the expression of intestinal Th2 cytokines in allergic mice.Mice were fed with OVA and curcumin as depicted in Fig 1A. (A-H) Expression of jejunal mRNA for various cytokines is shown. Data are representative of 2 independent experiments. * = p<0.05; ** = p<0.01.
Mentions: In order to examine whether curcumin suppresses local Th2 responses in the intestines, the jejunae of experimental animals was examined for the expression of the cytokines IL-4, IL-13, IL-5, IL-9, IL-33, IL-10, IL-17, and IFN-γ. As expected, the expression of the classic Th2 cytokines IL-4, IL-5, IL-13, IL-9, and IL-10 was significantly increased in the jejunum of OVA-challenged BALB/c mice compared with saline-treated controls. (Fig 3A–3D and 3F) In contrast, the expression of these cytokines was reduced in the intestines of OVA-challenged, curcumin-treated animals (Fig 3A–3D and 3F). There were no differences in IL-33 or IFN-γ expression in both groups (Fig 3E and 3H). Interestingly however, the increased expression of Th2 cytokines induced by OVA-challenge was also accompanied by a decrease in the expression of the Th17 cytokine, IL-17, in allergic mice (Fig 3G). In contrast, no change in expression of IL-17 was observed in the intestines of OVA-challenged, curcumin-treated mice as compared to saline-sensitized, curcumin-treated control animals (Fig 3G). Taken together, these data suggest that curcumin modulates the T cell response to oral antigen by skewing it away from a Th2-dominated phenotype.

Bottom Line: In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation.Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs.In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical and Administrative Sciences, College of Pharmacy, Western New England University, Springfield, MA 01119, United States of America.

ABSTRACT
IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during allergic responses. We assessed whether curcumin ingestion during oral allergen exposure can modulate the development of food allergy using a murine model of ovalbumin (OVA)-induced intestinal anaphylaxis. Herein, we demonstrate that frequent ingestion of curcumin during oral OVA exposure inhibits the development of mastocytosis and intestinal anaphylaxis in OVA-challenged allergic mice. Intragastric (i.g.) exposure to OVA in sensitized BALB/c mice induced a robust IgE-mediated response accompanied by enhanced OVA-IgE levels, intestinal mastocytosis, elevated serum mMCP-1, and acute diarrhea. In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation. Furthermore, allergic diarrhea, mast cell activation and expansion, and Th2 responses were also suppressed in mice exposed to curcumin during the OVA-challenge phase alone, despite the presence of elevated levels of OVA-IgE, suggesting that curcumin may have a direct suppressive effect on intestinal mast cell activation and reverse food allergy symptoms in allergen-sensitized individuals. This was confirmed by observations that curcumin attenuated the expansion of both adoptively transferred bone marrow-derived mast cells (BMMCs), and inhibited their survival and activation during cell culture. Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs. In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.

No MeSH data available.


Related in: MedlinePlus