Limits...
Specialized Cortex Glial Cells Accumulate Lipid Droplets in Drosophila melanogaster.

Kis V, Barti B, Lippai M, Sass M - PLoS ONE (2015)

Bottom Line: Although the central nervous system contains the highest relative amount and the largest number of different lipid species, neither the spatial nor the temporal distribution of LDs has been described.Superficial cortex glial cells, combined with subperineurial glial cells, express the Drosophila fatty acid binding protein (Dfabp), as we have demonstrated through light- and electron microscopic immunocytochemistry.To the best of our best knowledge this is the first study that describes LD neuroanatomy in the Drosophila larval brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.

ABSTRACT
Lipid droplets (LDs) are common organelles of the majority of eukaryotic cell types. Their biological significance has been extensively studied in mammalian liver cells and white adipose tissue. Although the central nervous system contains the highest relative amount and the largest number of different lipid species, neither the spatial nor the temporal distribution of LDs has been described. In this study, we used the brain of the fruitfly, Drosophila melanogaster, to investigate the neuroanatomy of LDs. We demonstrated that LDs are exclusively localised in glial cells but not in neurons in the larval nervous system. We showed that the brain's LD pool, rather than being constant, changes dynamically during development and reaches its highest value at the beginning of metamorphosis. LDs are particularly enriched in cortex glial cells located close to the brain surface. These specialized superficial cortex glial cells contain the highest amount of LDs among glial cell types and encapsulate neuroblasts and their daughter cells. Superficial cortex glial cells, combined with subperineurial glial cells, express the Drosophila fatty acid binding protein (Dfabp), as we have demonstrated through light- and electron microscopic immunocytochemistry. To the best of our best knowledge this is the first study that describes LD neuroanatomy in the Drosophila larval brain.

No MeSH data available.


Schematic illustration of the relative distribution of lipid droplets between glial cell types and the localization of Dfabp.LDs are concentrated in large clusters in the perinuclear region of glial cells but are not present at all in neurons (N). Specialized superficial cortex glial cells (CG) insulating neuroblasts (NB) and their daughter cells (asterisks) accumulating the highest amount of LDs. Neighboring subperineurial cells (SPG) establish septate junctions (SJ), while SPG and superficial cortex glial cells are connected to each other through adherens junctions (AJ). The Drosophila fatty acid binding protein (Dfabp) is expressed in LD accumulating superficial cortex glial cells and subperineurial (SPG) cells, and is localized in the cytosol and in the nucleus. NL: neural lamella, PG: perineural glia, NG: neuropil glia, ax: axon.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493057&req=5

pone.0131250.g007: Schematic illustration of the relative distribution of lipid droplets between glial cell types and the localization of Dfabp.LDs are concentrated in large clusters in the perinuclear region of glial cells but are not present at all in neurons (N). Specialized superficial cortex glial cells (CG) insulating neuroblasts (NB) and their daughter cells (asterisks) accumulating the highest amount of LDs. Neighboring subperineurial cells (SPG) establish septate junctions (SJ), while SPG and superficial cortex glial cells are connected to each other through adherens junctions (AJ). The Drosophila fatty acid binding protein (Dfabp) is expressed in LD accumulating superficial cortex glial cells and subperineurial (SPG) cells, and is localized in the cytosol and in the nucleus. NL: neural lamella, PG: perineural glia, NG: neuropil glia, ax: axon.

Mentions: LDs are common organelles of eukaryotic cells, participating in a variety of cellular processes. Although lipids are highly enriched in the nervous system, the spatio-temporal distribution and physiological function of LDs in the developing brain is poorly understood. In this work, we demonstrated the highly specific spatio-temporal distribution of LDs in the nervous system of Drosophila. Key findings of our work are summarized in Fig 7.


Specialized Cortex Glial Cells Accumulate Lipid Droplets in Drosophila melanogaster.

Kis V, Barti B, Lippai M, Sass M - PLoS ONE (2015)

Schematic illustration of the relative distribution of lipid droplets between glial cell types and the localization of Dfabp.LDs are concentrated in large clusters in the perinuclear region of glial cells but are not present at all in neurons (N). Specialized superficial cortex glial cells (CG) insulating neuroblasts (NB) and their daughter cells (asterisks) accumulating the highest amount of LDs. Neighboring subperineurial cells (SPG) establish septate junctions (SJ), while SPG and superficial cortex glial cells are connected to each other through adherens junctions (AJ). The Drosophila fatty acid binding protein (Dfabp) is expressed in LD accumulating superficial cortex glial cells and subperineurial (SPG) cells, and is localized in the cytosol and in the nucleus. NL: neural lamella, PG: perineural glia, NG: neuropil glia, ax: axon.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493057&req=5

pone.0131250.g007: Schematic illustration of the relative distribution of lipid droplets between glial cell types and the localization of Dfabp.LDs are concentrated in large clusters in the perinuclear region of glial cells but are not present at all in neurons (N). Specialized superficial cortex glial cells (CG) insulating neuroblasts (NB) and their daughter cells (asterisks) accumulating the highest amount of LDs. Neighboring subperineurial cells (SPG) establish septate junctions (SJ), while SPG and superficial cortex glial cells are connected to each other through adherens junctions (AJ). The Drosophila fatty acid binding protein (Dfabp) is expressed in LD accumulating superficial cortex glial cells and subperineurial (SPG) cells, and is localized in the cytosol and in the nucleus. NL: neural lamella, PG: perineural glia, NG: neuropil glia, ax: axon.
Mentions: LDs are common organelles of eukaryotic cells, participating in a variety of cellular processes. Although lipids are highly enriched in the nervous system, the spatio-temporal distribution and physiological function of LDs in the developing brain is poorly understood. In this work, we demonstrated the highly specific spatio-temporal distribution of LDs in the nervous system of Drosophila. Key findings of our work are summarized in Fig 7.

Bottom Line: Although the central nervous system contains the highest relative amount and the largest number of different lipid species, neither the spatial nor the temporal distribution of LDs has been described.Superficial cortex glial cells, combined with subperineurial glial cells, express the Drosophila fatty acid binding protein (Dfabp), as we have demonstrated through light- and electron microscopic immunocytochemistry.To the best of our best knowledge this is the first study that describes LD neuroanatomy in the Drosophila larval brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.

ABSTRACT
Lipid droplets (LDs) are common organelles of the majority of eukaryotic cell types. Their biological significance has been extensively studied in mammalian liver cells and white adipose tissue. Although the central nervous system contains the highest relative amount and the largest number of different lipid species, neither the spatial nor the temporal distribution of LDs has been described. In this study, we used the brain of the fruitfly, Drosophila melanogaster, to investigate the neuroanatomy of LDs. We demonstrated that LDs are exclusively localised in glial cells but not in neurons in the larval nervous system. We showed that the brain's LD pool, rather than being constant, changes dynamically during development and reaches its highest value at the beginning of metamorphosis. LDs are particularly enriched in cortex glial cells located close to the brain surface. These specialized superficial cortex glial cells contain the highest amount of LDs among glial cell types and encapsulate neuroblasts and their daughter cells. Superficial cortex glial cells, combined with subperineurial glial cells, express the Drosophila fatty acid binding protein (Dfabp), as we have demonstrated through light- and electron microscopic immunocytochemistry. To the best of our best knowledge this is the first study that describes LD neuroanatomy in the Drosophila larval brain.

No MeSH data available.