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Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring.

Mennitti LV, Oyama LM, de Oliveira JL, Hachul AC, Santamarina AB, de Santana AA, Okuda MH, Ribeiro EB, Oller do Nascimento CM, Pisani LP - PLoS ONE (2015)

Bottom Line: We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group.Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET.These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, Santos, SP, Brazil.

ABSTRACT
Previously, we showed that oligofructose (10%) supplementation during pregnancy and lactation increased endotoxemia in 21-d-old pups. The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old offspring. On the first day of pregnancy, female Wistar rats were divided into the following groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. Serum TNF-α (tumor necrosis factor alpha) was assessed using a specific kit. Protein expression was determined by Western Blotting, and the relative mRNA levels were analyzed by RT-PCR (real-time polymerase chain reaction). We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group. Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET. These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group. In conclusion, supplementing the dam's diet with 10% of oligofructose during pregnancy and lactation, independent of hydrogenated vegetable fat addition, contributes to the increased pro-inflammatory status of 21-d-old offspring, possibly through the activation of the TLR4 (toll like receptor 4) pathway.

No MeSH data available.


Related in: MedlinePlus

Serum concentration of TNF-α.C—male offspring of dams fed control diet; CF—male offspring of dams fed control diet supplemented with oligofructose; T—male offspring of dams fed diet enriched with hydrogenated vegetable fat; TF—male offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value (C group presented one outlier sample). Data are means ± SEMs. *p≤ 0.05 versus C. $p≤ 0.05 versus T. #p≤ 0.05 versus TF.
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pone.0132038.g001: Serum concentration of TNF-α.C—male offspring of dams fed control diet; CF—male offspring of dams fed control diet supplemented with oligofructose; T—male offspring of dams fed diet enriched with hydrogenated vegetable fat; TF—male offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value (C group presented one outlier sample). Data are means ± SEMs. *p≤ 0.05 versus C. $p≤ 0.05 versus T. #p≤ 0.05 versus TF.

Mentions: In order to investigate the effects of maternal supplementation with 10% of oligofructose during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old male offspring, we evaluated the TNF-α serum concentration, mRNA and protein levels of receptors involved in the inflammatory process (TLR4, TNFR1, IL-6R and ADIPOR1/ADIPOR2) of different tissues and protein expression of phosphorylated form of transcriptional factor NFκB (p50 and/or p65 subunits) in liver and RET. The results demonstrated a higher serum concentration of TNF-α in the TF group when compared with the T (p<0.05), C (p<0.01) and CF (p<0.05) groups (Fig 1).


Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring.

Mennitti LV, Oyama LM, de Oliveira JL, Hachul AC, Santamarina AB, de Santana AA, Okuda MH, Ribeiro EB, Oller do Nascimento CM, Pisani LP - PLoS ONE (2015)

Serum concentration of TNF-α.C—male offspring of dams fed control diet; CF—male offspring of dams fed control diet supplemented with oligofructose; T—male offspring of dams fed diet enriched with hydrogenated vegetable fat; TF—male offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value (C group presented one outlier sample). Data are means ± SEMs. *p≤ 0.05 versus C. $p≤ 0.05 versus T. #p≤ 0.05 versus TF.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4493056&req=5

pone.0132038.g001: Serum concentration of TNF-α.C—male offspring of dams fed control diet; CF—male offspring of dams fed control diet supplemented with oligofructose; T—male offspring of dams fed diet enriched with hydrogenated vegetable fat; TF—male offspring of dams fed diet enriched with hydrogenated vegetable fat supplemented with oligofructose. The number in parentheses refers to the sample value (C group presented one outlier sample). Data are means ± SEMs. *p≤ 0.05 versus C. $p≤ 0.05 versus T. #p≤ 0.05 versus TF.
Mentions: In order to investigate the effects of maternal supplementation with 10% of oligofructose during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old male offspring, we evaluated the TNF-α serum concentration, mRNA and protein levels of receptors involved in the inflammatory process (TLR4, TNFR1, IL-6R and ADIPOR1/ADIPOR2) of different tissues and protein expression of phosphorylated form of transcriptional factor NFκB (p50 and/or p65 subunits) in liver and RET. The results demonstrated a higher serum concentration of TNF-α in the TF group when compared with the T (p<0.05), C (p<0.01) and CF (p<0.05) groups (Fig 1).

Bottom Line: We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group.Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET.These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, Santos, SP, Brazil.

ABSTRACT
Previously, we showed that oligofructose (10%) supplementation during pregnancy and lactation increased endotoxemia in 21-d-old pups. The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old offspring. On the first day of pregnancy, female Wistar rats were divided into the following groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. Serum TNF-α (tumor necrosis factor alpha) was assessed using a specific kit. Protein expression was determined by Western Blotting, and the relative mRNA levels were analyzed by RT-PCR (real-time polymerase chain reaction). We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group. Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET. These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group. In conclusion, supplementing the dam's diet with 10% of oligofructose during pregnancy and lactation, independent of hydrogenated vegetable fat addition, contributes to the increased pro-inflammatory status of 21-d-old offspring, possibly through the activation of the TLR4 (toll like receptor 4) pathway.

No MeSH data available.


Related in: MedlinePlus