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Analysis of Differentially Expressed Genes Associated with Coronatine-Induced Laticifer Differentiation in the Rubber Tree by Subtractive Hybridization Suppression.

Zhang SX, Wu SH, Chen YY, Tian WM - PLoS ONE (2015)

Bottom Line: Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree.The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development.It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Hainan University, Haikou, Hainan, 570228, China; Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan, 571737, China.

ABSTRACT
The secondary laticifer in the secondary phloem is differentiated from the vascular cambia of the rubber tree (Hevea brasiliensis Muell. Arg.). The number of secondary laticifers is closely related to the rubber yield potential of Hevea. Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree. In the present study, an experimental system of coronatine-induced laticifer differentiation was developed to perform SSH identification of genes with differential expression. A total of 528 positive clones were obtained by blue-white screening, of which 248 clones came from the forward SSH library while 280 clones came from the reverse SSH library. Approximately 215 of the 248 clones and 171 of the 280 clones contained cDNA inserts by colony PCR screening. A total of 286 of the 386 ESTs were detected to be differentially expressed by reverse northern blot and sequenced. Approximately 147 unigenes with an average length of 497 bp from the forward and 109 unigenes with an average length of 514 bp from the reverse SSH libraries were assembled and annotated. The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development. It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

No MeSH data available.


The GO standard analysis of 286 genes from the forward and reverse SSH libraries by BGI WEGO.
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pone.0132070.g003: The GO standard analysis of 286 genes from the forward and reverse SSH libraries by BGI WEGO.

Mentions: Gene Ontology [14] conventions were used to provide descriptions of the gene products associated with molecular functions, cellular components and biological processes [19]. The functional classification of the 236 unigenes was performed by the GO convention (http://geneontology.org/). Fig 3 was drawn in Web Gene Ontology Annotation Plotting (BGI WEGO) (http://wego.genomics.org.cn/cgi-bin/wego/index.pl) to show the percentages of the subtractive unigenes in the three GO categories. (1) Approximately 54 and 17 unigenes were classified into 5 categories of the cellular component in the forward and reverse SSH libraries. Among them, unigenes encoding for cytochrome f, cytochrome b6 and ORF 143 were only present in the forward SSH library. (2) Approximately 67 and 35 unigenes were classified into 8 categories of molecular functions. The largest proportion of molecular functions were categorized by binding (31 and 17 unigenes) and catalytic activity (24 and 9 unigenes). Interestingly, antioxidant activity (1 unigene: predicted protein) and transporter activity (3 unigenes: vesicle docking protein P115, pyrophosphate-energized membrane proton pump 3 and PPase) were only noted in the forward SSH library; however, transcription regulator activity (1 unigene: Hevea brasiliensis microsatellite Hbtnr-23) and translation regulator activity (3 unigenes were all translation initiation factor, EIF) were annotated in the reverse SSH library. (3) Approximately 73 and 44 unigenes were classified into 8 categories of biological processes in the forward and reverse SSH libraries. Cellular processes (30 and 20 unigenes) and metabolic processes (31 and 20 unigenes) were the most frequently represented cellular components. Additionally, the 4 categories of biological processes: cellular component biogenesis (1 unigene: auxin-responsive protein IAA19), establishment of localization (3 unigenes: PPase, vesicle docking protein P115 and pyrophosphate-energized membrane proton pump 3), localization (3 unigenes: PPase, vesicle docking protein P115 and pyrophosphate-energized membrane proton pump 3) and response to stimulus (3 unigenes: predicted protein and HSP80) only appeared in the forward SSH library. Overall, the results showed that an obvious difference in several parts of the three GO categories existed between unigenes in the forward and reverse SSH libraries.


Analysis of Differentially Expressed Genes Associated with Coronatine-Induced Laticifer Differentiation in the Rubber Tree by Subtractive Hybridization Suppression.

Zhang SX, Wu SH, Chen YY, Tian WM - PLoS ONE (2015)

The GO standard analysis of 286 genes from the forward and reverse SSH libraries by BGI WEGO.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493031&req=5

pone.0132070.g003: The GO standard analysis of 286 genes from the forward and reverse SSH libraries by BGI WEGO.
Mentions: Gene Ontology [14] conventions were used to provide descriptions of the gene products associated with molecular functions, cellular components and biological processes [19]. The functional classification of the 236 unigenes was performed by the GO convention (http://geneontology.org/). Fig 3 was drawn in Web Gene Ontology Annotation Plotting (BGI WEGO) (http://wego.genomics.org.cn/cgi-bin/wego/index.pl) to show the percentages of the subtractive unigenes in the three GO categories. (1) Approximately 54 and 17 unigenes were classified into 5 categories of the cellular component in the forward and reverse SSH libraries. Among them, unigenes encoding for cytochrome f, cytochrome b6 and ORF 143 were only present in the forward SSH library. (2) Approximately 67 and 35 unigenes were classified into 8 categories of molecular functions. The largest proportion of molecular functions were categorized by binding (31 and 17 unigenes) and catalytic activity (24 and 9 unigenes). Interestingly, antioxidant activity (1 unigene: predicted protein) and transporter activity (3 unigenes: vesicle docking protein P115, pyrophosphate-energized membrane proton pump 3 and PPase) were only noted in the forward SSH library; however, transcription regulator activity (1 unigene: Hevea brasiliensis microsatellite Hbtnr-23) and translation regulator activity (3 unigenes were all translation initiation factor, EIF) were annotated in the reverse SSH library. (3) Approximately 73 and 44 unigenes were classified into 8 categories of biological processes in the forward and reverse SSH libraries. Cellular processes (30 and 20 unigenes) and metabolic processes (31 and 20 unigenes) were the most frequently represented cellular components. Additionally, the 4 categories of biological processes: cellular component biogenesis (1 unigene: auxin-responsive protein IAA19), establishment of localization (3 unigenes: PPase, vesicle docking protein P115 and pyrophosphate-energized membrane proton pump 3), localization (3 unigenes: PPase, vesicle docking protein P115 and pyrophosphate-energized membrane proton pump 3) and response to stimulus (3 unigenes: predicted protein and HSP80) only appeared in the forward SSH library. Overall, the results showed that an obvious difference in several parts of the three GO categories existed between unigenes in the forward and reverse SSH libraries.

Bottom Line: Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree.The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development.It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Hainan University, Haikou, Hainan, 570228, China; Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan, 571737, China.

ABSTRACT
The secondary laticifer in the secondary phloem is differentiated from the vascular cambia of the rubber tree (Hevea brasiliensis Muell. Arg.). The number of secondary laticifers is closely related to the rubber yield potential of Hevea. Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree. In the present study, an experimental system of coronatine-induced laticifer differentiation was developed to perform SSH identification of genes with differential expression. A total of 528 positive clones were obtained by blue-white screening, of which 248 clones came from the forward SSH library while 280 clones came from the reverse SSH library. Approximately 215 of the 248 clones and 171 of the 280 clones contained cDNA inserts by colony PCR screening. A total of 286 of the 386 ESTs were detected to be differentially expressed by reverse northern blot and sequenced. Approximately 147 unigenes with an average length of 497 bp from the forward and 109 unigenes with an average length of 514 bp from the reverse SSH libraries were assembled and annotated. The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development. It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

No MeSH data available.