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Analysis of Differentially Expressed Genes Associated with Coronatine-Induced Laticifer Differentiation in the Rubber Tree by Subtractive Hybridization Suppression.

Zhang SX, Wu SH, Chen YY, Tian WM - PLoS ONE (2015)

Bottom Line: Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree.The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development.It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Hainan University, Haikou, Hainan, 570228, China; Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan, 571737, China.

ABSTRACT
The secondary laticifer in the secondary phloem is differentiated from the vascular cambia of the rubber tree (Hevea brasiliensis Muell. Arg.). The number of secondary laticifers is closely related to the rubber yield potential of Hevea. Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree. In the present study, an experimental system of coronatine-induced laticifer differentiation was developed to perform SSH identification of genes with differential expression. A total of 528 positive clones were obtained by blue-white screening, of which 248 clones came from the forward SSH library while 280 clones came from the reverse SSH library. Approximately 215 of the 248 clones and 171 of the 280 clones contained cDNA inserts by colony PCR screening. A total of 286 of the 386 ESTs were detected to be differentially expressed by reverse northern blot and sequenced. Approximately 147 unigenes with an average length of 497 bp from the forward and 109 unigenes with an average length of 514 bp from the reverse SSH libraries were assembled and annotated. The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development. It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

No MeSH data available.


Diagram of Hevea epicormic shoots (A) and the cross-sections of bark (B-C) showing the sites of chemical application and secondary laticifer differentiation.The gray arrow in panel A indicates the site of chemical application and sampling. Panel B, treated with 20 μM coronatine (COR). Panel C, treated with water. EU, extension unit. White arrows show the primary laticifers. Black arrows show the secondary laticifers. Ca, cambium; St, sieve tube. Bars = 100 μm.
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pone.0132070.g001: Diagram of Hevea epicormic shoots (A) and the cross-sections of bark (B-C) showing the sites of chemical application and secondary laticifer differentiation.The gray arrow in panel A indicates the site of chemical application and sampling. Panel B, treated with 20 μM coronatine (COR). Panel C, treated with water. EU, extension unit. White arrows show the primary laticifers. Black arrows show the secondary laticifers. Ca, cambium; St, sieve tube. Bars = 100 μm.

Mentions: Plantlets of the rubber tree clone CATAS 7-33-97 budded on rootstocks were grown in the nursery of Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences (CATAS) on Hainan Island, P. R. China. The plantlets were pruned each year, and epicormic shoots were developed from the latent buds of the pruned stems. The epicormic shoots flush five to six times a year; therefore, such a shoot consists of a series of foliage clusters, separated by leafless lengths of stem. Each of these morphologically distinct growth increments represents a growth flush and is referred to as an extension unit (EU) [1] (Fig 1A). Fig 1A illustrates the sites of chemical application and sampling.


Analysis of Differentially Expressed Genes Associated with Coronatine-Induced Laticifer Differentiation in the Rubber Tree by Subtractive Hybridization Suppression.

Zhang SX, Wu SH, Chen YY, Tian WM - PLoS ONE (2015)

Diagram of Hevea epicormic shoots (A) and the cross-sections of bark (B-C) showing the sites of chemical application and secondary laticifer differentiation.The gray arrow in panel A indicates the site of chemical application and sampling. Panel B, treated with 20 μM coronatine (COR). Panel C, treated with water. EU, extension unit. White arrows show the primary laticifers. Black arrows show the secondary laticifers. Ca, cambium; St, sieve tube. Bars = 100 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493031&req=5

pone.0132070.g001: Diagram of Hevea epicormic shoots (A) and the cross-sections of bark (B-C) showing the sites of chemical application and secondary laticifer differentiation.The gray arrow in panel A indicates the site of chemical application and sampling. Panel B, treated with 20 μM coronatine (COR). Panel C, treated with water. EU, extension unit. White arrows show the primary laticifers. Black arrows show the secondary laticifers. Ca, cambium; St, sieve tube. Bars = 100 μm.
Mentions: Plantlets of the rubber tree clone CATAS 7-33-97 budded on rootstocks were grown in the nursery of Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences (CATAS) on Hainan Island, P. R. China. The plantlets were pruned each year, and epicormic shoots were developed from the latent buds of the pruned stems. The epicormic shoots flush five to six times a year; therefore, such a shoot consists of a series of foliage clusters, separated by leafless lengths of stem. Each of these morphologically distinct growth increments represents a growth flush and is referred to as an extension unit (EU) [1] (Fig 1A). Fig 1A illustrates the sites of chemical application and sampling.

Bottom Line: Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree.The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development.It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Hainan University, Haikou, Hainan, 570228, China; Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan, 571737, China.

ABSTRACT
The secondary laticifer in the secondary phloem is differentiated from the vascular cambia of the rubber tree (Hevea brasiliensis Muell. Arg.). The number of secondary laticifers is closely related to the rubber yield potential of Hevea. Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree. In the present study, an experimental system of coronatine-induced laticifer differentiation was developed to perform SSH identification of genes with differential expression. A total of 528 positive clones were obtained by blue-white screening, of which 248 clones came from the forward SSH library while 280 clones came from the reverse SSH library. Approximately 215 of the 248 clones and 171 of the 280 clones contained cDNA inserts by colony PCR screening. A total of 286 of the 386 ESTs were detected to be differentially expressed by reverse northern blot and sequenced. Approximately 147 unigenes with an average length of 497 bp from the forward and 109 unigenes with an average length of 514 bp from the reverse SSH libraries were assembled and annotated. The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development. It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

No MeSH data available.