Limits...
Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus

Quantification of hyphae, yeast and epithelial changes.(A) Scores and medians of the number of hyphae and yeast derived from single and mixed infection. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0009), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter. (B) Number of epithelial changes and medians observed in candidosis microscopic lesions on the tongue dorsum of mice inoculated with Candida monospecies and multi-species suspensions. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0013), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493022&req=5

pone.0131700.g010: Quantification of hyphae, yeast and epithelial changes.(A) Scores and medians of the number of hyphae and yeast derived from single and mixed infection. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0009), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter. (B) Number of epithelial changes and medians observed in candidosis microscopic lesions on the tongue dorsum of mice inoculated with Candida monospecies and multi-species suspensions. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0013), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter.

Mentions: In the analysis for quantification of yeasts/hyphae and epithelium alterations, the group with single infection by C. albicans obtained higher medians than the groups with mixed infection formed by C. albicans-C. krusei or by C. albicans-C. glabrata. In contrast, yeasts and hyphae were not found in the groups inoculated with monotypic suspensions of C. krusei or C. glabrata. Moreover, few epithelium alterations were found in these groups. These findings are consistent with the macroscopic results obtained in this study, which demonstrated that these non-albicans species are not capable of causing infection in the proposed model, being only able to colonize the oral cavity of mice (Fig 10).


Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Quantification of hyphae, yeast and epithelial changes.(A) Scores and medians of the number of hyphae and yeast derived from single and mixed infection. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0009), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter. (B) Number of epithelial changes and medians observed in candidosis microscopic lesions on the tongue dorsum of mice inoculated with Candida monospecies and multi-species suspensions. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0013), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493022&req=5

pone.0131700.g010: Quantification of hyphae, yeast and epithelial changes.(A) Scores and medians of the number of hyphae and yeast derived from single and mixed infection. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0009), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter. (B) Number of epithelial changes and medians observed in candidosis microscopic lesions on the tongue dorsum of mice inoculated with Candida monospecies and multi-species suspensions. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0013), with variations between C. albicans monospecies and multi-species groups, and similarities amid the latter.
Mentions: In the analysis for quantification of yeasts/hyphae and epithelium alterations, the group with single infection by C. albicans obtained higher medians than the groups with mixed infection formed by C. albicans-C. krusei or by C. albicans-C. glabrata. In contrast, yeasts and hyphae were not found in the groups inoculated with monotypic suspensions of C. krusei or C. glabrata. Moreover, few epithelium alterations were found in these groups. These findings are consistent with the macroscopic results obtained in this study, which demonstrated that these non-albicans species are not capable of causing infection in the proposed model, being only able to colonize the oral cavity of mice (Fig 10).

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus