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Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus

Candida hyphae and yeast on the tongue dorsum of mice.(A) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin. PAS; original magnification: 200X. (B) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and polymorphonuclear leukocytes forming intraepithelial microabscesses. PAS; magnification: 630X. C) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. krusei multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and spongiosis in the epithelium. PAS; magnification: 200X. D) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. glabrata multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin and in the epithelium. PAS; magnification: 400X.
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pone.0131700.g008: Candida hyphae and yeast on the tongue dorsum of mice.(A) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin. PAS; original magnification: 200X. (B) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and polymorphonuclear leukocytes forming intraepithelial microabscesses. PAS; magnification: 630X. C) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. krusei multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and spongiosis in the epithelium. PAS; magnification: 200X. D) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. glabrata multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin and in the epithelium. PAS; magnification: 400X.

Mentions: After that, the tongues were submitted to microscopic analysis and we verified the presence of yeast and hyphae in the keratinized layer of the tongue dorsum, specifically concentrated on conical papillae. In the areas with yeast and hyphae, we also found polymorphonuclear leukocytes forming intraepithelial microabscesses and cells of inflammatory infiltrate in the connective tissue (Fig 8). Several lesions were also observed in the epithelium such as desquamation, loss of filiform papillae, loss of stratification, epithelial hyperplasia, exocytosis, spongiosis, acantholysis, hyperkeratosis and basal layer (Fig 9).


Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Candida hyphae and yeast on the tongue dorsum of mice.(A) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin. PAS; original magnification: 200X. (B) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and polymorphonuclear leukocytes forming intraepithelial microabscesses. PAS; magnification: 630X. C) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. krusei multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and spongiosis in the epithelium. PAS; magnification: 200X. D) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. glabrata multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin and in the epithelium. PAS; magnification: 400X.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493022&req=5

pone.0131700.g008: Candida hyphae and yeast on the tongue dorsum of mice.(A) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin. PAS; original magnification: 200X. (B) Sagittal incision of the tongue dorsum of mice infected with C. albicans monospecies inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and polymorphonuclear leukocytes forming intraepithelial microabscesses. PAS; magnification: 630X. C) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. krusei multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin, and spongiosis in the epithelium. PAS; magnification: 200X. D) Sagittal incision of the tongue dorsum of mice infected with C. albicans and C. glabrata multi-species inoculum, displaying the presence of yeast and hyphae (↓) in keratin and in the epithelium. PAS; magnification: 400X.
Mentions: After that, the tongues were submitted to microscopic analysis and we verified the presence of yeast and hyphae in the keratinized layer of the tongue dorsum, specifically concentrated on conical papillae. In the areas with yeast and hyphae, we also found polymorphonuclear leukocytes forming intraepithelial microabscesses and cells of inflammatory infiltrate in the connective tissue (Fig 8). Several lesions were also observed in the epithelium such as desquamation, loss of filiform papillae, loss of stratification, epithelial hyperplasia, exocytosis, spongiosis, acantholysis, hyperkeratosis and basal layer (Fig 9).

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus