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Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus

Macroscopic analysis of candidiasis lesions.Scores and medians obtained from the macroscopic examination of the tongue dorsum of groups infected with C. albicans monospecies, C. krusei monospecies, C. glabrata monospecies, C. albicans-C. krusei multi-species and C. albicans-C. glabrata multi-species. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0001), with similarities between C. albicans monospecies and multi-species groups, and variations when compared with C. krusei and C. glabrata monospecies groups.
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pone.0131700.g007: Macroscopic analysis of candidiasis lesions.Scores and medians obtained from the macroscopic examination of the tongue dorsum of groups infected with C. albicans monospecies, C. krusei monospecies, C. glabrata monospecies, C. albicans-C. krusei multi-species and C. albicans-C. glabrata multi-species. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0001), with similarities between C. albicans monospecies and multi-species groups, and variations when compared with C. krusei and C. glabrata monospecies groups.

Mentions: In order to evaluate the development of oral candidiasis in mice, the clinical aspect of tongue dorsum was analysed during the experiments. In the macroscopic analysis of mice with single infection, the animals infected with C. albicans presented lesions typical of candidosis on their tongue dorsum. These lesions were characterized by white patches with areas of papillary atrophy and presence of pseudomembrane. On the other hand, monospecies groups formed by C. krusei or C. glabrata did not present macroscopic candidosis lesions on the tongue dorsum, suggesting that non-albicans species were not able to instigate the development such lesions, despite being capable of colonizing the oral cavities of mice. These macroscopic lesions were quantified and we observed that the group with single infection by C. albicans had a higher median than the groups infected with mixed species, although no significant statistical difference was found between groups (Fig 7).


Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Macroscopic analysis of candidiasis lesions.Scores and medians obtained from the macroscopic examination of the tongue dorsum of groups infected with C. albicans monospecies, C. krusei monospecies, C. glabrata monospecies, C. albicans-C. krusei multi-species and C. albicans-C. glabrata multi-species. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0001), with similarities between C. albicans monospecies and multi-species groups, and variations when compared with C. krusei and C. glabrata monospecies groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493022&req=5

pone.0131700.g007: Macroscopic analysis of candidiasis lesions.Scores and medians obtained from the macroscopic examination of the tongue dorsum of groups infected with C. albicans monospecies, C. krusei monospecies, C. glabrata monospecies, C. albicans-C. krusei multi-species and C. albicans-C. glabrata multi-species. Kruskal-Wallis and Dunn test: significant statistical differences were confirmed between the groups (p = 0.0001), with similarities between C. albicans monospecies and multi-species groups, and variations when compared with C. krusei and C. glabrata monospecies groups.
Mentions: In order to evaluate the development of oral candidiasis in mice, the clinical aspect of tongue dorsum was analysed during the experiments. In the macroscopic analysis of mice with single infection, the animals infected with C. albicans presented lesions typical of candidosis on their tongue dorsum. These lesions were characterized by white patches with areas of papillary atrophy and presence of pseudomembrane. On the other hand, monospecies groups formed by C. krusei or C. glabrata did not present macroscopic candidosis lesions on the tongue dorsum, suggesting that non-albicans species were not able to instigate the development such lesions, despite being capable of colonizing the oral cavities of mice. These macroscopic lesions were quantified and we observed that the group with single infection by C. albicans had a higher median than the groups infected with mixed species, although no significant statistical difference was found between groups (Fig 7).

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus