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Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus

Quantification of Candida cells adhered to the swab.Mean and standard deviation of the CFU/mL (Log) of C. albicans in monotypic suspension, C. krusei in monotypic suspension, C. glabrata in monotypic suspension, C. albicans in heteroptypic suspension with C. krusei, C. albicans in heteroptypic suspension with C. glabrata, C. krusei in heteroptypic suspension with C. albicans, and C. glabrata in heteroptypic suspension with C. albicans. ANOVA test: there are no statistically significant differences between the groups.
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pone.0131700.g005: Quantification of Candida cells adhered to the swab.Mean and standard deviation of the CFU/mL (Log) of C. albicans in monotypic suspension, C. krusei in monotypic suspension, C. glabrata in monotypic suspension, C. albicans in heteroptypic suspension with C. krusei, C. albicans in heteroptypic suspension with C. glabrata, C. krusei in heteroptypic suspension with C. albicans, and C. glabrata in heteroptypic suspension with C. albicans. ANOVA test: there are no statistically significant differences between the groups.

Mentions: The interaction Candida study established in G. mellonella larvae was moved to a model of experimental oral candidiasis in immunosuppressed mice. Monotypic and heterotypic suspensions of Candida species were inoculated in the oral cavity of mice using a swab previously soaked in the Candida suspensions. Initialy, we did an experiment to confirm the concentration of Candida cells adhered to the swab. C. albicans, C. krusei and C. glabrata showed the same capacity to adhere to the swab when they were soaked in both monotypic and heterotypic suspensions (Fig 5).


Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Quantification of Candida cells adhered to the swab.Mean and standard deviation of the CFU/mL (Log) of C. albicans in monotypic suspension, C. krusei in monotypic suspension, C. glabrata in monotypic suspension, C. albicans in heteroptypic suspension with C. krusei, C. albicans in heteroptypic suspension with C. glabrata, C. krusei in heteroptypic suspension with C. albicans, and C. glabrata in heteroptypic suspension with C. albicans. ANOVA test: there are no statistically significant differences between the groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493022&req=5

pone.0131700.g005: Quantification of Candida cells adhered to the swab.Mean and standard deviation of the CFU/mL (Log) of C. albicans in monotypic suspension, C. krusei in monotypic suspension, C. glabrata in monotypic suspension, C. albicans in heteroptypic suspension with C. krusei, C. albicans in heteroptypic suspension with C. glabrata, C. krusei in heteroptypic suspension with C. albicans, and C. glabrata in heteroptypic suspension with C. albicans. ANOVA test: there are no statistically significant differences between the groups.
Mentions: The interaction Candida study established in G. mellonella larvae was moved to a model of experimental oral candidiasis in immunosuppressed mice. Monotypic and heterotypic suspensions of Candida species were inoculated in the oral cavity of mice using a swab previously soaked in the Candida suspensions. Initialy, we did an experiment to confirm the concentration of Candida cells adhered to the swab. C. albicans, C. krusei and C. glabrata showed the same capacity to adhere to the swab when they were soaked in both monotypic and heterotypic suspensions (Fig 5).

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus