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Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


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Quantification of fungal cells in G. mellonella hemolymph.Mean and standard deviation of CFU/mL (Log) values in G. mellonella hemolymph at various time periods of experimental infection. (A) C. albicans CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 12 h period (ANOVA and Tukey’s test). C. albicans was not quantified in G. mellonella hemolymph at 18 and 24 h because all larvae had perished by those times. (B) C. krusei CFU/mL measurements: significant statistical differences were observed between groups in the 12, 18 and 24 h periods (Student t test). (C) C. glabrata CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 24 h period (Student t test).
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pone.0131700.g004: Quantification of fungal cells in G. mellonella hemolymph.Mean and standard deviation of CFU/mL (Log) values in G. mellonella hemolymph at various time periods of experimental infection. (A) C. albicans CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 12 h period (ANOVA and Tukey’s test). C. albicans was not quantified in G. mellonella hemolymph at 18 and 24 h because all larvae had perished by those times. (B) C. krusei CFU/mL measurements: significant statistical differences were observed between groups in the 12, 18 and 24 h periods (Student t test). (C) C. glabrata CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 24 h period (Student t test).

Mentions: Candida CFU/mL measurements of G. mellonella hemolymph displayed similar growth patterns in the infection by C. albicans in both single and mixed infections at 0, 4 and 8 hours after fungal inoculation. However, a significant statistical difference between the groups was found at 12h post-infection (Fig 4A), where C. albicans presented noticeably poorer growth in the interaction group when compared to the monotypic group.


Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.

Rossoni RD, Barbosa JO, Vilela SF, dos Santos JD, de Barros PP, Prata MC, Anbinder AL, Fuchs BB, Jorge AO, Mylonakis E, Junqueira JC - PLoS ONE (2015)

Quantification of fungal cells in G. mellonella hemolymph.Mean and standard deviation of CFU/mL (Log) values in G. mellonella hemolymph at various time periods of experimental infection. (A) C. albicans CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 12 h period (ANOVA and Tukey’s test). C. albicans was not quantified in G. mellonella hemolymph at 18 and 24 h because all larvae had perished by those times. (B) C. krusei CFU/mL measurements: significant statistical differences were observed between groups in the 12, 18 and 24 h periods (Student t test). (C) C. glabrata CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 24 h period (Student t test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493022&req=5

pone.0131700.g004: Quantification of fungal cells in G. mellonella hemolymph.Mean and standard deviation of CFU/mL (Log) values in G. mellonella hemolymph at various time periods of experimental infection. (A) C. albicans CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 12 h period (ANOVA and Tukey’s test). C. albicans was not quantified in G. mellonella hemolymph at 18 and 24 h because all larvae had perished by those times. (B) C. krusei CFU/mL measurements: significant statistical differences were observed between groups in the 12, 18 and 24 h periods (Student t test). (C) C. glabrata CFU/mL measurements: significant statistical differences were observed between monotypic and heterotypic groups in the 24 h period (Student t test).
Mentions: Candida CFU/mL measurements of G. mellonella hemolymph displayed similar growth patterns in the infection by C. albicans in both single and mixed infections at 0, 4 and 8 hours after fungal inoculation. However, a significant statistical difference between the groups was found at 12h post-infection (Fig 4A), where C. albicans presented noticeably poorer growth in the interaction group when compared to the monotypic group.

Bottom Line: CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively.After that, we expanded this study for in vivo host models of experimental candidiasis.In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Oral Diagnosis, Univ Estadual Paulista/UNESP, São José dos Campos, São Paulo, Brazil.

ABSTRACT
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

No MeSH data available.


Related in: MedlinePlus