Limits...
Preconditioning L6 Muscle Cells with Naringin Ameliorates Oxidative Stress and Increases Glucose Uptake.

Dhanya R, Arun KB, Nisha VM, Syama HP, Nisha P, Santhosh Kumar TR, Jayamurthy P - PLoS ONE (2015)

Bottom Line: Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes.Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro.Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

View Article: PubMed Central - PubMed

Affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram-695019, Kerala, India.

ABSTRACT
Enhanced oxidative stress contributes to pathological changes in diabetes and its complications. Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes. Herein, we have investigated Naringin mediated regulation of glutathione (GSH) & intracellular free radical levels and modulation of glucose uptake under oxidative stress in L6 cell lines. The results from the study demonstrated a marked decrease in glutathione with a subsequent increase in free radical levels, which was reversed by the pretreatment of Naringin. We also observed that the increased malondialdehyde level, the marker of lipid peroxidation on induction of oxidative stress was retrieved on Naringin pretreatment. Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro. Furthermore, we observed a twofold increase in uptake of fluorescent labeled glucose namely 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) on Naringin treatment in differentiated L6 myoblast. The increased uptake of 2-NBDG by L6 myotubes may be attributed due to the enhanced translocation of GLUT4. Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

No MeSH data available.


Related in: MedlinePlus

GLUT4 upregulation on Naringin pretreatment.Immunofluorescence assay visualized upregulation of GLUT4 in differentiated L6 myoblast. High resolution confocal images (40X) of Untreated L6 myotubes, L6 myotubes treated with TBHP, Rosiglitazone (100 nM) and Naringin (100 μM, 24 h). Scale bar corresponds to 10μM. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments. Significance test between various groups were determined by using one way ANOVA followed by Duncan’s multiple range test. * P≤0.05 versus control; #P≤0.05 versus TBHP.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4492986&req=5

pone.0132429.g005: GLUT4 upregulation on Naringin pretreatment.Immunofluorescence assay visualized upregulation of GLUT4 in differentiated L6 myoblast. High resolution confocal images (40X) of Untreated L6 myotubes, L6 myotubes treated with TBHP, Rosiglitazone (100 nM) and Naringin (100 μM, 24 h). Scale bar corresponds to 10μM. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments. Significance test between various groups were determined by using one way ANOVA followed by Duncan’s multiple range test. * P≤0.05 versus control; #P≤0.05 versus TBHP.

Mentions: Immunofluorescence staining revealed an upregulation of GLUT4 transporter protein on pretreatment of Naringin (100 μM) which was comparable to that of positive control as shown in Fig 5 (P≥0.05). The results obtained by quantifying immunologically labeled GLUT4 at the surface of intact cells correlates with that of glucose uptake, suggesting that the induction of GLUT4 by pretreatment of Naringin is likely responsible for enhanced glucose uptake exhibited by L6 myotubes. TBHP exposure to cells did not induce any changes in GLUT4 translocation.


Preconditioning L6 Muscle Cells with Naringin Ameliorates Oxidative Stress and Increases Glucose Uptake.

Dhanya R, Arun KB, Nisha VM, Syama HP, Nisha P, Santhosh Kumar TR, Jayamurthy P - PLoS ONE (2015)

GLUT4 upregulation on Naringin pretreatment.Immunofluorescence assay visualized upregulation of GLUT4 in differentiated L6 myoblast. High resolution confocal images (40X) of Untreated L6 myotubes, L6 myotubes treated with TBHP, Rosiglitazone (100 nM) and Naringin (100 μM, 24 h). Scale bar corresponds to 10μM. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments. Significance test between various groups were determined by using one way ANOVA followed by Duncan’s multiple range test. * P≤0.05 versus control; #P≤0.05 versus TBHP.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492986&req=5

pone.0132429.g005: GLUT4 upregulation on Naringin pretreatment.Immunofluorescence assay visualized upregulation of GLUT4 in differentiated L6 myoblast. High resolution confocal images (40X) of Untreated L6 myotubes, L6 myotubes treated with TBHP, Rosiglitazone (100 nM) and Naringin (100 μM, 24 h). Scale bar corresponds to 10μM. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments. Significance test between various groups were determined by using one way ANOVA followed by Duncan’s multiple range test. * P≤0.05 versus control; #P≤0.05 versus TBHP.
Mentions: Immunofluorescence staining revealed an upregulation of GLUT4 transporter protein on pretreatment of Naringin (100 μM) which was comparable to that of positive control as shown in Fig 5 (P≥0.05). The results obtained by quantifying immunologically labeled GLUT4 at the surface of intact cells correlates with that of glucose uptake, suggesting that the induction of GLUT4 by pretreatment of Naringin is likely responsible for enhanced glucose uptake exhibited by L6 myotubes. TBHP exposure to cells did not induce any changes in GLUT4 translocation.

Bottom Line: Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes.Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro.Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

View Article: PubMed Central - PubMed

Affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram-695019, Kerala, India.

ABSTRACT
Enhanced oxidative stress contributes to pathological changes in diabetes and its complications. Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes. Herein, we have investigated Naringin mediated regulation of glutathione (GSH) & intracellular free radical levels and modulation of glucose uptake under oxidative stress in L6 cell lines. The results from the study demonstrated a marked decrease in glutathione with a subsequent increase in free radical levels, which was reversed by the pretreatment of Naringin. We also observed that the increased malondialdehyde level, the marker of lipid peroxidation on induction of oxidative stress was retrieved on Naringin pretreatment. Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro. Furthermore, we observed a twofold increase in uptake of fluorescent labeled glucose namely 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) on Naringin treatment in differentiated L6 myoblast. The increased uptake of 2-NBDG by L6 myotubes may be attributed due to the enhanced translocation of GLUT4. Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

No MeSH data available.


Related in: MedlinePlus