Limits...
Preconditioning L6 Muscle Cells with Naringin Ameliorates Oxidative Stress and Increases Glucose Uptake.

Dhanya R, Arun KB, Nisha VM, Syama HP, Nisha P, Santhosh Kumar TR, Jayamurthy P - PLoS ONE (2015)

Bottom Line: Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes.Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro.Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

View Article: PubMed Central - PubMed

Affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram-695019, Kerala, India.

ABSTRACT
Enhanced oxidative stress contributes to pathological changes in diabetes and its complications. Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes. Herein, we have investigated Naringin mediated regulation of glutathione (GSH) & intracellular free radical levels and modulation of glucose uptake under oxidative stress in L6 cell lines. The results from the study demonstrated a marked decrease in glutathione with a subsequent increase in free radical levels, which was reversed by the pretreatment of Naringin. We also observed that the increased malondialdehyde level, the marker of lipid peroxidation on induction of oxidative stress was retrieved on Naringin pretreatment. Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro. Furthermore, we observed a twofold increase in uptake of fluorescent labeled glucose namely 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) on Naringin treatment in differentiated L6 myoblast. The increased uptake of 2-NBDG by L6 myotubes may be attributed due to the enhanced translocation of GLUT4. Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

No MeSH data available.


Related in: MedlinePlus

Antiglycation activity of Naringin.Antiglycation activity of Naringin at three different concentrations (1, 10 & 100 μM). Ascorbic acid was used as standard. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4492986&req=5

pone.0132429.g003: Antiglycation activity of Naringin.Antiglycation activity of Naringin at three different concentrations (1, 10 & 100 μM). Ascorbic acid was used as standard. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.

Mentions: Non-enzymatic glycosylation between reducing sugar and protein results in the formation of advanced glycation end products (AGEs), which is involved in diabetic complications. Thus, compounds that inhibit the formation of AGEs are believed to have therapeutic potentials in patients with diabetes. Naringin showed a marked decline in in vitro glycation of proteins in a dose dependent manner (Fig 3). Naringin at 100μM showed an inhibition of 40%. Ascorbic acid was used as a positive control (IC50 -30 μM).


Preconditioning L6 Muscle Cells with Naringin Ameliorates Oxidative Stress and Increases Glucose Uptake.

Dhanya R, Arun KB, Nisha VM, Syama HP, Nisha P, Santhosh Kumar TR, Jayamurthy P - PLoS ONE (2015)

Antiglycation activity of Naringin.Antiglycation activity of Naringin at three different concentrations (1, 10 & 100 μM). Ascorbic acid was used as standard. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492986&req=5

pone.0132429.g003: Antiglycation activity of Naringin.Antiglycation activity of Naringin at three different concentrations (1, 10 & 100 μM). Ascorbic acid was used as standard. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.
Mentions: Non-enzymatic glycosylation between reducing sugar and protein results in the formation of advanced glycation end products (AGEs), which is involved in diabetic complications. Thus, compounds that inhibit the formation of AGEs are believed to have therapeutic potentials in patients with diabetes. Naringin showed a marked decline in in vitro glycation of proteins in a dose dependent manner (Fig 3). Naringin at 100μM showed an inhibition of 40%. Ascorbic acid was used as a positive control (IC50 -30 μM).

Bottom Line: Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes.Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro.Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

View Article: PubMed Central - PubMed

Affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram-695019, Kerala, India.

ABSTRACT
Enhanced oxidative stress contributes to pathological changes in diabetes and its complications. Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes. Herein, we have investigated Naringin mediated regulation of glutathione (GSH) & intracellular free radical levels and modulation of glucose uptake under oxidative stress in L6 cell lines. The results from the study demonstrated a marked decrease in glutathione with a subsequent increase in free radical levels, which was reversed by the pretreatment of Naringin. We also observed that the increased malondialdehyde level, the marker of lipid peroxidation on induction of oxidative stress was retrieved on Naringin pretreatment. Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro. Furthermore, we observed a twofold increase in uptake of fluorescent labeled glucose namely 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) on Naringin treatment in differentiated L6 myoblast. The increased uptake of 2-NBDG by L6 myotubes may be attributed due to the enhanced translocation of GLUT4. Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

No MeSH data available.


Related in: MedlinePlus