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Preconditioning L6 Muscle Cells with Naringin Ameliorates Oxidative Stress and Increases Glucose Uptake.

Dhanya R, Arun KB, Nisha VM, Syama HP, Nisha P, Santhosh Kumar TR, Jayamurthy P - PLoS ONE (2015)

Bottom Line: Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes.Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro.Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

View Article: PubMed Central - PubMed

Affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram-695019, Kerala, India.

ABSTRACT
Enhanced oxidative stress contributes to pathological changes in diabetes and its complications. Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes. Herein, we have investigated Naringin mediated regulation of glutathione (GSH) & intracellular free radical levels and modulation of glucose uptake under oxidative stress in L6 cell lines. The results from the study demonstrated a marked decrease in glutathione with a subsequent increase in free radical levels, which was reversed by the pretreatment of Naringin. We also observed that the increased malondialdehyde level, the marker of lipid peroxidation on induction of oxidative stress was retrieved on Naringin pretreatment. Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro. Furthermore, we observed a twofold increase in uptake of fluorescent labeled glucose namely 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) on Naringin treatment in differentiated L6 myoblast. The increased uptake of 2-NBDG by L6 myotubes may be attributed due to the enhanced translocation of GLUT4. Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity in cultured L6 myoblast.(A) Effect of TBHP on cell viability was evaluated based on concentration as well as period of incubation. (B) Cytotoxicity of Naringin was determined after 24 h preincubation in L6 myoblast. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.
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pone.0132429.g001: Cytotoxicity in cultured L6 myoblast.(A) Effect of TBHP on cell viability was evaluated based on concentration as well as period of incubation. (B) Cytotoxicity of Naringin was determined after 24 h preincubation in L6 myoblast. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.

Mentions: The cytotoxicity of TBHP was standardized based on concentration as well as period of incubation. TBHP and Naringin at 100 μM was found to be less than 20 (Fig 1A) and 15% toxic (Fig 1B) for a period of 3 and 24 h respectively. These concentrations were taken for further studies.


Preconditioning L6 Muscle Cells with Naringin Ameliorates Oxidative Stress and Increases Glucose Uptake.

Dhanya R, Arun KB, Nisha VM, Syama HP, Nisha P, Santhosh Kumar TR, Jayamurthy P - PLoS ONE (2015)

Cytotoxicity in cultured L6 myoblast.(A) Effect of TBHP on cell viability was evaluated based on concentration as well as period of incubation. (B) Cytotoxicity of Naringin was determined after 24 h preincubation in L6 myoblast. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492986&req=5

pone.0132429.g001: Cytotoxicity in cultured L6 myoblast.(A) Effect of TBHP on cell viability was evaluated based on concentration as well as period of incubation. (B) Cytotoxicity of Naringin was determined after 24 h preincubation in L6 myoblast. Each value represents mean ± SD (standard deviation) from triplicate measurements (n = 3) of three different experiments.
Mentions: The cytotoxicity of TBHP was standardized based on concentration as well as period of incubation. TBHP and Naringin at 100 μM was found to be less than 20 (Fig 1A) and 15% toxic (Fig 1B) for a period of 3 and 24 h respectively. These concentrations were taken for further studies.

Bottom Line: Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes.Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro.Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

View Article: PubMed Central - PubMed

Affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram-695019, Kerala, India.

ABSTRACT
Enhanced oxidative stress contributes to pathological changes in diabetes and its complications. Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes. Herein, we have investigated Naringin mediated regulation of glutathione (GSH) & intracellular free radical levels and modulation of glucose uptake under oxidative stress in L6 cell lines. The results from the study demonstrated a marked decrease in glutathione with a subsequent increase in free radical levels, which was reversed by the pretreatment of Naringin. We also observed that the increased malondialdehyde level, the marker of lipid peroxidation on induction of oxidative stress was retrieved on Naringin pretreatment. Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro. Furthermore, we observed a twofold increase in uptake of fluorescent labeled glucose namely 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) on Naringin treatment in differentiated L6 myoblast. The increased uptake of 2-NBDG by L6 myotubes may be attributed due to the enhanced translocation of GLUT4. Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control.

No MeSH data available.


Related in: MedlinePlus