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A Novel Lipase as Aquafeed Additive for Warm-Water Aquaculture.

Ran C, He S, Yang Y, Huang L, Zhou Z - PLoS ONE (2015)

Bottom Line: Results showed that supplementation of LipG1 significantly improved the gut and heptaopancreas lipase activity of fish fed with palm oil diet.Consistently, improved feed conversion ratio and growth performance were recorded in the LipG1 feeding group, to levels comparable to the group of fish fed with soybean oil diet.Collectively, LipG1 exhibited good potential as an aquafeed additive enzyme, and deserves further characterization as the representative of a novel group of lipases.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.

ABSTRACT
A novel Acinetobacter lipase gene lipG1 was cloned from DNA extracted from intestinal sample of common carp (Cyprinus carpio), and expressed in E. coli BL21. The encoded protein was 406 amino acids in length. Phylogenetic analysis indicated that LipG1 and its relatives comprised a novel group of true lipases produced by Gram-negative bacteria. LipG1 showed maximal activity at 40℃ and pH 8.0 when pNP decanoate (C10) was used as the substrate, and remained high activity between 20℃ and 35℃. Activity of the lipase was promoted by Ca2+ and Mg2+, and inhibited by Zn2+ and Cu2+. Moreover, LipG1 is stable with proteases, most commercial detergents and organic solvents. Substrate specificity test indicated that LipG1 can hydrolyse pNP esters with acyl chain length from C2 to C16, with preference for medium-chain pNP esters (C8, C10). Lastly, LipG1 was evaluated as an aquafeed additive for juvenile common carp (Cyprinus carpio). Results showed that supplementation of LipG1 significantly improved the gut and heptaopancreas lipase activity of fish fed with palm oil diet. Consistently, improved feed conversion ratio and growth performance were recorded in the LipG1 feeding group, to levels comparable to the group of fish fed with soybean oil diet. Collectively, LipG1 exhibited good potential as an aquafeed additive enzyme, and deserves further characterization as the representative of a novel group of lipases.

No MeSH data available.


Related in: MedlinePlus

Cluster analysis of the adhesive gut bacterial communities of common carp (Cyprinus carpio).
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pone.0132049.g003: Cluster analysis of the adhesive gut bacterial communities of common carp (Cyprinus carpio).

Mentions: The 16S rRNA gene V3 region PCR-DGGE fingerprints of the adhesive intestinal microbiota of different groups are shown in S1 Fig Most of the OTUs of the intestinal microbiota of common carp were assigned to the Proteobacteria (17 OTU) and Firmicutes (4 OTU) (S3 Table). The gut microbiota was affected by dietary lipid sources, as the microbiota of palm oil diet associated groups were clearly distinguishable from the microbiota of the soybean oil group (Fig 3). Lipase supplementation didn’t exert significant change to the intestinal microbiota, and the intestinal microbial profiles of all the lipase supplement groups were similar (Fig 3).


A Novel Lipase as Aquafeed Additive for Warm-Water Aquaculture.

Ran C, He S, Yang Y, Huang L, Zhou Z - PLoS ONE (2015)

Cluster analysis of the adhesive gut bacterial communities of common carp (Cyprinus carpio).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492967&req=5

pone.0132049.g003: Cluster analysis of the adhesive gut bacterial communities of common carp (Cyprinus carpio).
Mentions: The 16S rRNA gene V3 region PCR-DGGE fingerprints of the adhesive intestinal microbiota of different groups are shown in S1 Fig Most of the OTUs of the intestinal microbiota of common carp were assigned to the Proteobacteria (17 OTU) and Firmicutes (4 OTU) (S3 Table). The gut microbiota was affected by dietary lipid sources, as the microbiota of palm oil diet associated groups were clearly distinguishable from the microbiota of the soybean oil group (Fig 3). Lipase supplementation didn’t exert significant change to the intestinal microbiota, and the intestinal microbial profiles of all the lipase supplement groups were similar (Fig 3).

Bottom Line: Results showed that supplementation of LipG1 significantly improved the gut and heptaopancreas lipase activity of fish fed with palm oil diet.Consistently, improved feed conversion ratio and growth performance were recorded in the LipG1 feeding group, to levels comparable to the group of fish fed with soybean oil diet.Collectively, LipG1 exhibited good potential as an aquafeed additive enzyme, and deserves further characterization as the representative of a novel group of lipases.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.

ABSTRACT
A novel Acinetobacter lipase gene lipG1 was cloned from DNA extracted from intestinal sample of common carp (Cyprinus carpio), and expressed in E. coli BL21. The encoded protein was 406 amino acids in length. Phylogenetic analysis indicated that LipG1 and its relatives comprised a novel group of true lipases produced by Gram-negative bacteria. LipG1 showed maximal activity at 40℃ and pH 8.0 when pNP decanoate (C10) was used as the substrate, and remained high activity between 20℃ and 35℃. Activity of the lipase was promoted by Ca2+ and Mg2+, and inhibited by Zn2+ and Cu2+. Moreover, LipG1 is stable with proteases, most commercial detergents and organic solvents. Substrate specificity test indicated that LipG1 can hydrolyse pNP esters with acyl chain length from C2 to C16, with preference for medium-chain pNP esters (C8, C10). Lastly, LipG1 was evaluated as an aquafeed additive for juvenile common carp (Cyprinus carpio). Results showed that supplementation of LipG1 significantly improved the gut and heptaopancreas lipase activity of fish fed with palm oil diet. Consistently, improved feed conversion ratio and growth performance were recorded in the LipG1 feeding group, to levels comparable to the group of fish fed with soybean oil diet. Collectively, LipG1 exhibited good potential as an aquafeed additive enzyme, and deserves further characterization as the representative of a novel group of lipases.

No MeSH data available.


Related in: MedlinePlus