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The Light Wavelength Affects the Ontogeny of Clock Gene Expression and Activity Rhythms in Zebrafish Larvae.

Di Rosa V, Frigato E, López-Olmeda JF, Sánchez-Vázquez FJ, Bertolucci C - PLoS ONE (2015)

Bottom Line: The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms.Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf).Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia Spain.

ABSTRACT
Light plays a key role in synchronizing rhythms and setting the phase of early development. However, to date, little is known about the impact of light wavelengths during the ontogeny of the molecular clock and the behavioural rhythmicity. The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms. For this purpose, 4 groups of zebrafish embryo/larvae were raised from 0 to 7 days post-fertilization (dpf) under the following lighting conditions: three groups maintained under light:dark (LD) cycles with white (full visible spectrum, LDW), blue (LDB), or red light (LDR), and one group raised under constant darkness (DD). The results showed that lighting conditions influenced activity rhythms. Larvae were arrhythmic under DD, while under LD cycles they developed wavelength-dependent daily activity rhythms which appeared earlier under LDB (4 dpf) than under LDW or LDR (5 dpf). The results also revealed that development and lighting conditions influenced clock gene expression. While clock1 rhythmic expression appeared in all lighting conditions at 7 dpf, per1b, per2 and dbp showed daily variations already at 3 dpf. Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf). Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR. These results emphasize the importance of lighting conditions and wavelengths during early development for the ontogeny of daily rhythms of gene expression and how these rhythms are reflected on the behavioural rhythmicity of zebrafish larvae.

No MeSH data available.


Related in: MedlinePlus

Daily activity under different lighting conditions from 2 to 7 dpf.Data are expressed as mean ± SEM. Letters indicate statistical differences between the different days for each lighting condition (one-way ANOVA; Tukey’s post-hoc test, p<0.05). Symbols indicate statistical differences between the lighting conditions (two-way ANOVA; Tukey’s post-hoc test, p<0.05).
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pone.0132235.g003: Daily activity under different lighting conditions from 2 to 7 dpf.Data are expressed as mean ± SEM. Letters indicate statistical differences between the different days for each lighting condition (one-way ANOVA; Tukey’s post-hoc test, p<0.05). Symbols indicate statistical differences between the lighting conditions (two-way ANOVA; Tukey’s post-hoc test, p<0.05).

Mentions: All groups showed an increase in the total daily locomotor activity throughout development (two-way ANOVA, p<0.05) (Fig 3). Larvae kept under LDB and LDR showed an increase in the total daily activity from 4 dpf, whereas a significant increase in activity under LDW and DD occurred at 5 dpf (Figs 1 and 3). Considering the whole period of recording (5 days, from 2 to 7 dpf), larvae reared under LDW and LDB displayed significantly higher overall activity than larvae under LDR and DD (LDW: 60.17 m; LDB: 56.51 m; LDR: 42.05 m; two-way ANOVA, p<0.05). Furthermore, larvae reared in DD showed lower total activity with respect larvae reared in the other lighting conditions (DD: 27.36 m; two-way ANOVA, p<0.05).


The Light Wavelength Affects the Ontogeny of Clock Gene Expression and Activity Rhythms in Zebrafish Larvae.

Di Rosa V, Frigato E, López-Olmeda JF, Sánchez-Vázquez FJ, Bertolucci C - PLoS ONE (2015)

Daily activity under different lighting conditions from 2 to 7 dpf.Data are expressed as mean ± SEM. Letters indicate statistical differences between the different days for each lighting condition (one-way ANOVA; Tukey’s post-hoc test, p<0.05). Symbols indicate statistical differences between the lighting conditions (two-way ANOVA; Tukey’s post-hoc test, p<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492954&req=5

pone.0132235.g003: Daily activity under different lighting conditions from 2 to 7 dpf.Data are expressed as mean ± SEM. Letters indicate statistical differences between the different days for each lighting condition (one-way ANOVA; Tukey’s post-hoc test, p<0.05). Symbols indicate statistical differences between the lighting conditions (two-way ANOVA; Tukey’s post-hoc test, p<0.05).
Mentions: All groups showed an increase in the total daily locomotor activity throughout development (two-way ANOVA, p<0.05) (Fig 3). Larvae kept under LDB and LDR showed an increase in the total daily activity from 4 dpf, whereas a significant increase in activity under LDW and DD occurred at 5 dpf (Figs 1 and 3). Considering the whole period of recording (5 days, from 2 to 7 dpf), larvae reared under LDW and LDB displayed significantly higher overall activity than larvae under LDR and DD (LDW: 60.17 m; LDB: 56.51 m; LDR: 42.05 m; two-way ANOVA, p<0.05). Furthermore, larvae reared in DD showed lower total activity with respect larvae reared in the other lighting conditions (DD: 27.36 m; two-way ANOVA, p<0.05).

Bottom Line: The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms.Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf).Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia Spain.

ABSTRACT
Light plays a key role in synchronizing rhythms and setting the phase of early development. However, to date, little is known about the impact of light wavelengths during the ontogeny of the molecular clock and the behavioural rhythmicity. The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms. For this purpose, 4 groups of zebrafish embryo/larvae were raised from 0 to 7 days post-fertilization (dpf) under the following lighting conditions: three groups maintained under light:dark (LD) cycles with white (full visible spectrum, LDW), blue (LDB), or red light (LDR), and one group raised under constant darkness (DD). The results showed that lighting conditions influenced activity rhythms. Larvae were arrhythmic under DD, while under LD cycles they developed wavelength-dependent daily activity rhythms which appeared earlier under LDB (4 dpf) than under LDW or LDR (5 dpf). The results also revealed that development and lighting conditions influenced clock gene expression. While clock1 rhythmic expression appeared in all lighting conditions at 7 dpf, per1b, per2 and dbp showed daily variations already at 3 dpf. Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf). Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR. These results emphasize the importance of lighting conditions and wavelengths during early development for the ontogeny of daily rhythms of gene expression and how these rhythms are reflected on the behavioural rhythmicity of zebrafish larvae.

No MeSH data available.


Related in: MedlinePlus