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The Light Wavelength Affects the Ontogeny of Clock Gene Expression and Activity Rhythms in Zebrafish Larvae.

Di Rosa V, Frigato E, López-Olmeda JF, Sánchez-Vázquez FJ, Bertolucci C - PLoS ONE (2015)

Bottom Line: The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms.Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf).Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia Spain.

ABSTRACT
Light plays a key role in synchronizing rhythms and setting the phase of early development. However, to date, little is known about the impact of light wavelengths during the ontogeny of the molecular clock and the behavioural rhythmicity. The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms. For this purpose, 4 groups of zebrafish embryo/larvae were raised from 0 to 7 days post-fertilization (dpf) under the following lighting conditions: three groups maintained under light:dark (LD) cycles with white (full visible spectrum, LDW), blue (LDB), or red light (LDR), and one group raised under constant darkness (DD). The results showed that lighting conditions influenced activity rhythms. Larvae were arrhythmic under DD, while under LD cycles they developed wavelength-dependent daily activity rhythms which appeared earlier under LDB (4 dpf) than under LDW or LDR (5 dpf). The results also revealed that development and lighting conditions influenced clock gene expression. While clock1 rhythmic expression appeared in all lighting conditions at 7 dpf, per1b, per2 and dbp showed daily variations already at 3 dpf. Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf). Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR. These results emphasize the importance of lighting conditions and wavelengths during early development for the ontogeny of daily rhythms of gene expression and how these rhythms are reflected on the behavioural rhythmicity of zebrafish larvae.

No MeSH data available.


Related in: MedlinePlus

Mean waveforms of locomotor activity under different lighting conditions from 2 to 7 dpf.Larvae reared under different light conditions (A: LDW, n = 35, B: LDB, n = 30, C: LDR, n = 30, D: DD, n = 45). Vertical axis represents activity (m/10 min) and horizontal axis zeitgeber/circadian time (ZT/CT). Bars above each panel indicate the lighting conditions [black bars indicate darkness, white bars indicate white light (LDW), blue bars indicate blue light (LDB), and red bars indicate red light (LDR)] and the day post-fertilization (dpf). Data are expressed as mean ± SEM.
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pone.0132235.g001: Mean waveforms of locomotor activity under different lighting conditions from 2 to 7 dpf.Larvae reared under different light conditions (A: LDW, n = 35, B: LDB, n = 30, C: LDR, n = 30, D: DD, n = 45). Vertical axis represents activity (m/10 min) and horizontal axis zeitgeber/circadian time (ZT/CT). Bars above each panel indicate the lighting conditions [black bars indicate darkness, white bars indicate white light (LDW), blue bars indicate blue light (LDB), and red bars indicate red light (LDR)] and the day post-fertilization (dpf). Data are expressed as mean ± SEM.

Mentions: All larvae hatched between 2 and 3 dpf. At 4 dpf, larvae from LDB group started to display a daily rhythm of locomotor activity (Fig 1B; Cosinor, p<0.001). Daily rhythms of activity became significant at 5 dpf in LDW and LDR groups (Fig 1A and 1C; Cosinor, p<0.001 and p<0.01, respectively). Larvae reared under DD were arrhythmic during all the days recorded (Fig 1D; Cosinor, p>0.1). Larvae from all LD groups display the typical diurnal pattern of zebrafish, with higher activity (>65%) during the light phase.


The Light Wavelength Affects the Ontogeny of Clock Gene Expression and Activity Rhythms in Zebrafish Larvae.

Di Rosa V, Frigato E, López-Olmeda JF, Sánchez-Vázquez FJ, Bertolucci C - PLoS ONE (2015)

Mean waveforms of locomotor activity under different lighting conditions from 2 to 7 dpf.Larvae reared under different light conditions (A: LDW, n = 35, B: LDB, n = 30, C: LDR, n = 30, D: DD, n = 45). Vertical axis represents activity (m/10 min) and horizontal axis zeitgeber/circadian time (ZT/CT). Bars above each panel indicate the lighting conditions [black bars indicate darkness, white bars indicate white light (LDW), blue bars indicate blue light (LDB), and red bars indicate red light (LDR)] and the day post-fertilization (dpf). Data are expressed as mean ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492954&req=5

pone.0132235.g001: Mean waveforms of locomotor activity under different lighting conditions from 2 to 7 dpf.Larvae reared under different light conditions (A: LDW, n = 35, B: LDB, n = 30, C: LDR, n = 30, D: DD, n = 45). Vertical axis represents activity (m/10 min) and horizontal axis zeitgeber/circadian time (ZT/CT). Bars above each panel indicate the lighting conditions [black bars indicate darkness, white bars indicate white light (LDW), blue bars indicate blue light (LDB), and red bars indicate red light (LDR)] and the day post-fertilization (dpf). Data are expressed as mean ± SEM.
Mentions: All larvae hatched between 2 and 3 dpf. At 4 dpf, larvae from LDB group started to display a daily rhythm of locomotor activity (Fig 1B; Cosinor, p<0.001). Daily rhythms of activity became significant at 5 dpf in LDW and LDR groups (Fig 1A and 1C; Cosinor, p<0.001 and p<0.01, respectively). Larvae reared under DD were arrhythmic during all the days recorded (Fig 1D; Cosinor, p>0.1). Larvae from all LD groups display the typical diurnal pattern of zebrafish, with higher activity (>65%) during the light phase.

Bottom Line: The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms.Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf).Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia Spain.

ABSTRACT
Light plays a key role in synchronizing rhythms and setting the phase of early development. However, to date, little is known about the impact of light wavelengths during the ontogeny of the molecular clock and the behavioural rhythmicity. The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms. For this purpose, 4 groups of zebrafish embryo/larvae were raised from 0 to 7 days post-fertilization (dpf) under the following lighting conditions: three groups maintained under light:dark (LD) cycles with white (full visible spectrum, LDW), blue (LDB), or red light (LDR), and one group raised under constant darkness (DD). The results showed that lighting conditions influenced activity rhythms. Larvae were arrhythmic under DD, while under LD cycles they developed wavelength-dependent daily activity rhythms which appeared earlier under LDB (4 dpf) than under LDW or LDR (5 dpf). The results also revealed that development and lighting conditions influenced clock gene expression. While clock1 rhythmic expression appeared in all lighting conditions at 7 dpf, per1b, per2 and dbp showed daily variations already at 3 dpf. Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf). Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR. These results emphasize the importance of lighting conditions and wavelengths during early development for the ontogeny of daily rhythms of gene expression and how these rhythms are reflected on the behavioural rhythmicity of zebrafish larvae.

No MeSH data available.


Related in: MedlinePlus