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Epigenetic Diversity of Clonal White Poplar (Populus alba L.) Populations: Could Methylation Support the Success of Vegetative Reproduction Strategy?

Guarino F, Cicatelli A, Brundu G, Heinze B, Castiglione S - PLoS ONE (2015)

Bottom Line: The variable epigenetic status of Sardinian white poplars revealed a decreased number of population clusters.Landscape genetics analyses clearly demonstrated that ramets of the same clone were differentially methylated in relation to their geographic position.Therefore, our data support the notion that studies on plant biodiversity should no longer be restricted to genetic aspects, especially in the case of vegetatively propagated plant species.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Chimica e Biologia, Università degli Studi di Salerno, Fisciano, Italia.

ABSTRACT
The widespread poplar populations of Sardinia are vegetatively propagated and live in different natural environments forming large monoclonal stands. The main goals of the present study were: i) to investigate/measure the epigenetic diversity of the poplar populations by determining their DNA methylation status; ii) to assess if and how methylation status influences population clustering; iii) to shed light on the changes that occur in the epigenome of ramets of the same poplar clone. To these purposes, 83 white poplar trees were sampled at different locations on the island of Sardinia. Methylation sensitive amplified polymorphism analysis was carried out on the genomic DNA extracted from leaves at the same juvenile stage. The study showed that the genetic biodiversity of poplars is quite limited but it is counterbalanced by epigenetic inter-population molecular variability. The comparison between MspI and HpaII DNA fragmentation profiles revealed that environmental conditions strongly influence hemi-methylation of the inner cytosine. The variable epigenetic status of Sardinian white poplars revealed a decreased number of population clusters. Landscape genetics analyses clearly demonstrated that ramets of the same clone were differentially methylated in relation to their geographic position. Therefore, our data support the notion that studies on plant biodiversity should no longer be restricted to genetic aspects, especially in the case of vegetatively propagated plant species.

No MeSH data available.


Related in: MedlinePlus

Representation of Principal Coordinate Analysis (PCoA) for epigenetic differentiation among groups.Different colours represent different populations, called a, b, c. The a, b, c, symbols indicate the position of the genetic centroids. Ellipses represent the average dispersion of those individual data points around their centre. The long axis of the ellipse shows the direction of maximum dispersion and the short axis, the direction of minimum dispersion. Fig 5A and 5B show MspI and HpaII results, respectively.
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pone.0131480.g005: Representation of Principal Coordinate Analysis (PCoA) for epigenetic differentiation among groups.Different colours represent different populations, called a, b, c. The a, b, c, symbols indicate the position of the genetic centroids. Ellipses represent the average dispersion of those individual data points around their centre. The long axis of the ellipse shows the direction of maximum dispersion and the short axis, the direction of minimum dispersion. Fig 5A and 5B show MspI and HpaII results, respectively.

Mentions: The Principal Coordinate Analysis (PCoA—Fig 5), calculated using the MspI and HpaII profiles (Fig 5A and 5B), showed how epigenetic diversity, regarding the hemi-methylation of outer cytosine, was larger than that revealed by the MspI profile (Table 4). The MspI PCoA showed that the three estimated populations were intersected and shared a large part of the ellipse areas (Fig 5B); on the contrary, the HpaII PCoA showed three clearly distinct populations with partial overlapping. The length of the major and minor axes of the ellipses, representing the dispersion degree of the trees, showed that the dispersion was lower in the case of HpaII data when compared to those produced by MspI digestion. This finding was further confirmed by C1 and C2 values, representing the percentage of the explained variance, which were much smaller in the case of HpaII PCoA.


Epigenetic Diversity of Clonal White Poplar (Populus alba L.) Populations: Could Methylation Support the Success of Vegetative Reproduction Strategy?

Guarino F, Cicatelli A, Brundu G, Heinze B, Castiglione S - PLoS ONE (2015)

Representation of Principal Coordinate Analysis (PCoA) for epigenetic differentiation among groups.Different colours represent different populations, called a, b, c. The a, b, c, symbols indicate the position of the genetic centroids. Ellipses represent the average dispersion of those individual data points around their centre. The long axis of the ellipse shows the direction of maximum dispersion and the short axis, the direction of minimum dispersion. Fig 5A and 5B show MspI and HpaII results, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492942&req=5

pone.0131480.g005: Representation of Principal Coordinate Analysis (PCoA) for epigenetic differentiation among groups.Different colours represent different populations, called a, b, c. The a, b, c, symbols indicate the position of the genetic centroids. Ellipses represent the average dispersion of those individual data points around their centre. The long axis of the ellipse shows the direction of maximum dispersion and the short axis, the direction of minimum dispersion. Fig 5A and 5B show MspI and HpaII results, respectively.
Mentions: The Principal Coordinate Analysis (PCoA—Fig 5), calculated using the MspI and HpaII profiles (Fig 5A and 5B), showed how epigenetic diversity, regarding the hemi-methylation of outer cytosine, was larger than that revealed by the MspI profile (Table 4). The MspI PCoA showed that the three estimated populations were intersected and shared a large part of the ellipse areas (Fig 5B); on the contrary, the HpaII PCoA showed three clearly distinct populations with partial overlapping. The length of the major and minor axes of the ellipses, representing the dispersion degree of the trees, showed that the dispersion was lower in the case of HpaII data when compared to those produced by MspI digestion. This finding was further confirmed by C1 and C2 values, representing the percentage of the explained variance, which were much smaller in the case of HpaII PCoA.

Bottom Line: The variable epigenetic status of Sardinian white poplars revealed a decreased number of population clusters.Landscape genetics analyses clearly demonstrated that ramets of the same clone were differentially methylated in relation to their geographic position.Therefore, our data support the notion that studies on plant biodiversity should no longer be restricted to genetic aspects, especially in the case of vegetatively propagated plant species.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Chimica e Biologia, Università degli Studi di Salerno, Fisciano, Italia.

ABSTRACT
The widespread poplar populations of Sardinia are vegetatively propagated and live in different natural environments forming large monoclonal stands. The main goals of the present study were: i) to investigate/measure the epigenetic diversity of the poplar populations by determining their DNA methylation status; ii) to assess if and how methylation status influences population clustering; iii) to shed light on the changes that occur in the epigenome of ramets of the same poplar clone. To these purposes, 83 white poplar trees were sampled at different locations on the island of Sardinia. Methylation sensitive amplified polymorphism analysis was carried out on the genomic DNA extracted from leaves at the same juvenile stage. The study showed that the genetic biodiversity of poplars is quite limited but it is counterbalanced by epigenetic inter-population molecular variability. The comparison between MspI and HpaII DNA fragmentation profiles revealed that environmental conditions strongly influence hemi-methylation of the inner cytosine. The variable epigenetic status of Sardinian white poplars revealed a decreased number of population clusters. Landscape genetics analyses clearly demonstrated that ramets of the same clone were differentially methylated in relation to their geographic position. Therefore, our data support the notion that studies on plant biodiversity should no longer be restricted to genetic aspects, especially in the case of vegetatively propagated plant species.

No MeSH data available.


Related in: MedlinePlus