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Differential Activation of Human Monocytes and Lymphocytes by Distinct Strains of Trypanosoma cruzi.

Magalhães LM, Viana A, Chiari E, Galvão LM, Gollob KJ, Dutra WO - PLoS Negl Trop Dis (2015)

Bottom Line: It is known that these DTUs have different geographical distribution, as well as biological features.TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively.These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT

Background: Trypanosoma cruzi strains are currently classified into six discrete typing units (DTUs) named TcI to VI. It is known that these DTUs have different geographical distribution, as well as biological features. TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively. Our hypothesis is that upon infection of human peripheral blood cells, Y strain (Tc II) and Col cl1.7 (Tc I), cause distinct immunological changes, which might influence the clinical course of Chagas disease.

Methodology/principal findings: We evaluated the infectivity of CFSE-stained trypomastigotes of Col cl1.7 and Y strain in human monocytes for 15 and 72 hours, and determined the immunological profile of lymphocytes and monocytes exposed to the different isolates using multiparameter flow cytometry. Our results showed a similar percentage and intensity of monocyte infection by Y and Col cl1.7. We also observed an increased expression of CD80 and CD86 by monocytes infected with Col cl1.7, but not Y strain. IL-10 was significantly higher in monocytes infected with Col cl1.7, as compared to Y strain. Moreover, infection with Col cl1.7, but not Y strain, led to an increased expression of IL-17 by CD8+ T cells. On the other hand, we observed a positive correlation between the expression of TNF-alpha and granzyme A only after infection with Y strain.

Conclusion/significance: Our study shows that while Col cl1.7 induces higher monocyte activation and, at the same time, production of IL-10, infection with Y strain leads to a lower monocyte activation but higher inflammatory profile. These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

No MeSH data available.


Related in: MedlinePlus

Correlation analysis of the expression of Granzyme A with the expression of TNF-alpha in CD8 T cells.Statistical significance (p value) is indicated in each graph together with the r2 value.
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pntd.0003816.g005: Correlation analysis of the expression of Granzyme A with the expression of TNF-alpha in CD8 T cells.Statistical significance (p value) is indicated in each graph together with the r2 value.

Mentions: We performed correlation analysis to determine whether the increase in expression of Granzyme A by CD8 T lymphocytes was associated with the expression of TNF-alpha by these cells. Our data showed a positive correlation between the expression of TNF-alpha and the expression of Granzyme A. Interestingly, this correlation was only observed after the infection with Y strain and not in media or infection with Col cl1.7 (Fig 5).


Differential Activation of Human Monocytes and Lymphocytes by Distinct Strains of Trypanosoma cruzi.

Magalhães LM, Viana A, Chiari E, Galvão LM, Gollob KJ, Dutra WO - PLoS Negl Trop Dis (2015)

Correlation analysis of the expression of Granzyme A with the expression of TNF-alpha in CD8 T cells.Statistical significance (p value) is indicated in each graph together with the r2 value.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492932&req=5

pntd.0003816.g005: Correlation analysis of the expression of Granzyme A with the expression of TNF-alpha in CD8 T cells.Statistical significance (p value) is indicated in each graph together with the r2 value.
Mentions: We performed correlation analysis to determine whether the increase in expression of Granzyme A by CD8 T lymphocytes was associated with the expression of TNF-alpha by these cells. Our data showed a positive correlation between the expression of TNF-alpha and the expression of Granzyme A. Interestingly, this correlation was only observed after the infection with Y strain and not in media or infection with Col cl1.7 (Fig 5).

Bottom Line: It is known that these DTUs have different geographical distribution, as well as biological features.TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively.These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT

Background: Trypanosoma cruzi strains are currently classified into six discrete typing units (DTUs) named TcI to VI. It is known that these DTUs have different geographical distribution, as well as biological features. TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively. Our hypothesis is that upon infection of human peripheral blood cells, Y strain (Tc II) and Col cl1.7 (Tc I), cause distinct immunological changes, which might influence the clinical course of Chagas disease.

Methodology/principal findings: We evaluated the infectivity of CFSE-stained trypomastigotes of Col cl1.7 and Y strain in human monocytes for 15 and 72 hours, and determined the immunological profile of lymphocytes and monocytes exposed to the different isolates using multiparameter flow cytometry. Our results showed a similar percentage and intensity of monocyte infection by Y and Col cl1.7. We also observed an increased expression of CD80 and CD86 by monocytes infected with Col cl1.7, but not Y strain. IL-10 was significantly higher in monocytes infected with Col cl1.7, as compared to Y strain. Moreover, infection with Col cl1.7, but not Y strain, led to an increased expression of IL-17 by CD8+ T cells. On the other hand, we observed a positive correlation between the expression of TNF-alpha and granzyme A only after infection with Y strain.

Conclusion/significance: Our study shows that while Col cl1.7 induces higher monocyte activation and, at the same time, production of IL-10, infection with Y strain leads to a lower monocyte activation but higher inflammatory profile. These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

No MeSH data available.


Related in: MedlinePlus