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Differential Activation of Human Monocytes and Lymphocytes by Distinct Strains of Trypanosoma cruzi.

Magalhães LM, Viana A, Chiari E, Galvão LM, Gollob KJ, Dutra WO - PLoS Negl Trop Dis (2015)

Bottom Line: It is known that these DTUs have different geographical distribution, as well as biological features.TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively.These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT

Background: Trypanosoma cruzi strains are currently classified into six discrete typing units (DTUs) named TcI to VI. It is known that these DTUs have different geographical distribution, as well as biological features. TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively. Our hypothesis is that upon infection of human peripheral blood cells, Y strain (Tc II) and Col cl1.7 (Tc I), cause distinct immunological changes, which might influence the clinical course of Chagas disease.

Methodology/principal findings: We evaluated the infectivity of CFSE-stained trypomastigotes of Col cl1.7 and Y strain in human monocytes for 15 and 72 hours, and determined the immunological profile of lymphocytes and monocytes exposed to the different isolates using multiparameter flow cytometry. Our results showed a similar percentage and intensity of monocyte infection by Y and Col cl1.7. We also observed an increased expression of CD80 and CD86 by monocytes infected with Col cl1.7, but not Y strain. IL-10 was significantly higher in monocytes infected with Col cl1.7, as compared to Y strain. Moreover, infection with Col cl1.7, but not Y strain, led to an increased expression of IL-17 by CD8+ T cells. On the other hand, we observed a positive correlation between the expression of TNF-alpha and granzyme A only after infection with Y strain.

Conclusion/significance: Our study shows that while Col cl1.7 induces higher monocyte activation and, at the same time, production of IL-10, infection with Y strain leads to a lower monocyte activation but higher inflammatory profile. These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

No MeSH data available.


Related in: MedlinePlus

Determination of the percentage of expression of the cytokines TNF-alpha, IL-12/IL-23p40 and IL-10 by human monocytes infected or not with different strains of T. cruzi.Results are expressed as average ± standard deviation. The symbol * indicates p < 0.05 between groups. Determination of percentage of (A) TNF-alpha in CFSE+ monocytes; (B) TNF-alpha in CFSE- monocytes; (C) IL-12/IL-23p40 in CFSE+ monocytes; (D) IL-12/IL-23p40 in CFSE- monocytes; (E) IL-10 in CFSE+ monocytes; (F) IL-10 in CFSE- monocytes; All analysis done after 15 hours of culture.
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pntd.0003816.g003: Determination of the percentage of expression of the cytokines TNF-alpha, IL-12/IL-23p40 and IL-10 by human monocytes infected or not with different strains of T. cruzi.Results are expressed as average ± standard deviation. The symbol * indicates p < 0.05 between groups. Determination of percentage of (A) TNF-alpha in CFSE+ monocytes; (B) TNF-alpha in CFSE- monocytes; (C) IL-12/IL-23p40 in CFSE+ monocytes; (D) IL-12/IL-23p40 in CFSE- monocytes; (E) IL-10 in CFSE+ monocytes; (F) IL-10 in CFSE- monocytes; All analysis done after 15 hours of culture.

Mentions: We questioned whether the monocyte activation triggered by infection had an influence on the expression of cytokines after 15 hours of infection with the Y strain or Col cl1.7. We observed a higher expression of TNF-alpha and IL-12/IL-23p40 in monocytes infected with the two strains compared to media control (Fig 3A and 3C, respectively). No changes were observed in non-infected monocytes (Fig 3B and 3D).


Differential Activation of Human Monocytes and Lymphocytes by Distinct Strains of Trypanosoma cruzi.

Magalhães LM, Viana A, Chiari E, Galvão LM, Gollob KJ, Dutra WO - PLoS Negl Trop Dis (2015)

Determination of the percentage of expression of the cytokines TNF-alpha, IL-12/IL-23p40 and IL-10 by human monocytes infected or not with different strains of T. cruzi.Results are expressed as average ± standard deviation. The symbol * indicates p < 0.05 between groups. Determination of percentage of (A) TNF-alpha in CFSE+ monocytes; (B) TNF-alpha in CFSE- monocytes; (C) IL-12/IL-23p40 in CFSE+ monocytes; (D) IL-12/IL-23p40 in CFSE- monocytes; (E) IL-10 in CFSE+ monocytes; (F) IL-10 in CFSE- monocytes; All analysis done after 15 hours of culture.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492932&req=5

pntd.0003816.g003: Determination of the percentage of expression of the cytokines TNF-alpha, IL-12/IL-23p40 and IL-10 by human monocytes infected or not with different strains of T. cruzi.Results are expressed as average ± standard deviation. The symbol * indicates p < 0.05 between groups. Determination of percentage of (A) TNF-alpha in CFSE+ monocytes; (B) TNF-alpha in CFSE- monocytes; (C) IL-12/IL-23p40 in CFSE+ monocytes; (D) IL-12/IL-23p40 in CFSE- monocytes; (E) IL-10 in CFSE+ monocytes; (F) IL-10 in CFSE- monocytes; All analysis done after 15 hours of culture.
Mentions: We questioned whether the monocyte activation triggered by infection had an influence on the expression of cytokines after 15 hours of infection with the Y strain or Col cl1.7. We observed a higher expression of TNF-alpha and IL-12/IL-23p40 in monocytes infected with the two strains compared to media control (Fig 3A and 3C, respectively). No changes were observed in non-infected monocytes (Fig 3B and 3D).

Bottom Line: It is known that these DTUs have different geographical distribution, as well as biological features.TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively.These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT

Background: Trypanosoma cruzi strains are currently classified into six discrete typing units (DTUs) named TcI to VI. It is known that these DTUs have different geographical distribution, as well as biological features. TcI and TcII are major DTUs found in patients from northern and southern Latin America, respectively. Our hypothesis is that upon infection of human peripheral blood cells, Y strain (Tc II) and Col cl1.7 (Tc I), cause distinct immunological changes, which might influence the clinical course of Chagas disease.

Methodology/principal findings: We evaluated the infectivity of CFSE-stained trypomastigotes of Col cl1.7 and Y strain in human monocytes for 15 and 72 hours, and determined the immunological profile of lymphocytes and monocytes exposed to the different isolates using multiparameter flow cytometry. Our results showed a similar percentage and intensity of monocyte infection by Y and Col cl1.7. We also observed an increased expression of CD80 and CD86 by monocytes infected with Col cl1.7, but not Y strain. IL-10 was significantly higher in monocytes infected with Col cl1.7, as compared to Y strain. Moreover, infection with Col cl1.7, but not Y strain, led to an increased expression of IL-17 by CD8+ T cells. On the other hand, we observed a positive correlation between the expression of TNF-alpha and granzyme A only after infection with Y strain.

Conclusion/significance: Our study shows that while Col cl1.7 induces higher monocyte activation and, at the same time, production of IL-10, infection with Y strain leads to a lower monocyte activation but higher inflammatory profile. These results show that TcI and TcII have a distinct immunological impact on human cells during early infection, which might influence disease progression.

No MeSH data available.


Related in: MedlinePlus