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Tocilizumab unmasks a stage-dependent interleukin-6 component in statin-induced apoptosis of metastatic melanoma cells.

Minichsdorfer C, Wasinger C, Sieczkowski E, Atil B, Hohenegger M - Melanoma Res. (2015)

Bottom Line: The effects of IL-6 were measured by western blots for STAT3 and Bcl-2 family proteins.This proapoptotic effect of IL-6 might be explained by a downregulation of Bcl-XL, observed only in WM35 cells.These results confirm that simvastatin facilitates apoptosis in combination with IL-6.

View Article: PubMed Central - PubMed

Affiliation: aDepartment of Oncology, Medicine I bCenter for Physiology and Pharmacology, Comprehensive Cancer Center, Institute of Pharmacology cDepartment of Neurology, Medical University of Vienna, Vienna, Austria.

No MeSH data available.


Related in: MedlinePlus

Simvastatin-induced caspase 3 activation in human melanoma cells. (a) The 518A2, A375, and WM35 cells were treated with the indicated concentrations of simvastatin for 48 h, lysed, and subjected to assessment of caspase 3 activity. Melanoma cells (b) 518A2, (c) A375, and (d) WM35 were treated for 48 h in the absence (CTL) and presence of 10 μmol/l simvastatin (Sim) and/or 20 ng/ml interleukin (IL)-6. Data points or bars represent the mean±SEM (n=3). Statistical significance versus control: **P<0.01; ***P<0.001, or versus simvastatin treatment: ###P<0.001; NS, not significant.
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Figure 4: Simvastatin-induced caspase 3 activation in human melanoma cells. (a) The 518A2, A375, and WM35 cells were treated with the indicated concentrations of simvastatin for 48 h, lysed, and subjected to assessment of caspase 3 activity. Melanoma cells (b) 518A2, (c) A375, and (d) WM35 were treated for 48 h in the absence (CTL) and presence of 10 μmol/l simvastatin (Sim) and/or 20 ng/ml interleukin (IL)-6. Data points or bars represent the mean±SEM (n=3). Statistical significance versus control: **P<0.01; ***P<0.001, or versus simvastatin treatment: ###P<0.001; NS, not significant.

Mentions: The executer caspase 3 mirrors the point of no return in the apoptotic machinery. For comparison, simvastatin-induced caspase 3 activation was evaluated in all three melanoma cell lines to corroborate apoptosis by another method (Fig. 4a).


Tocilizumab unmasks a stage-dependent interleukin-6 component in statin-induced apoptosis of metastatic melanoma cells.

Minichsdorfer C, Wasinger C, Sieczkowski E, Atil B, Hohenegger M - Melanoma Res. (2015)

Simvastatin-induced caspase 3 activation in human melanoma cells. (a) The 518A2, A375, and WM35 cells were treated with the indicated concentrations of simvastatin for 48 h, lysed, and subjected to assessment of caspase 3 activity. Melanoma cells (b) 518A2, (c) A375, and (d) WM35 were treated for 48 h in the absence (CTL) and presence of 10 μmol/l simvastatin (Sim) and/or 20 ng/ml interleukin (IL)-6. Data points or bars represent the mean±SEM (n=3). Statistical significance versus control: **P<0.01; ***P<0.001, or versus simvastatin treatment: ###P<0.001; NS, not significant.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492793&req=5

Figure 4: Simvastatin-induced caspase 3 activation in human melanoma cells. (a) The 518A2, A375, and WM35 cells were treated with the indicated concentrations of simvastatin for 48 h, lysed, and subjected to assessment of caspase 3 activity. Melanoma cells (b) 518A2, (c) A375, and (d) WM35 were treated for 48 h in the absence (CTL) and presence of 10 μmol/l simvastatin (Sim) and/or 20 ng/ml interleukin (IL)-6. Data points or bars represent the mean±SEM (n=3). Statistical significance versus control: **P<0.01; ***P<0.001, or versus simvastatin treatment: ###P<0.001; NS, not significant.
Mentions: The executer caspase 3 mirrors the point of no return in the apoptotic machinery. For comparison, simvastatin-induced caspase 3 activation was evaluated in all three melanoma cell lines to corroborate apoptosis by another method (Fig. 4a).

Bottom Line: The effects of IL-6 were measured by western blots for STAT3 and Bcl-2 family proteins.This proapoptotic effect of IL-6 might be explained by a downregulation of Bcl-XL, observed only in WM35 cells.These results confirm that simvastatin facilitates apoptosis in combination with IL-6.

View Article: PubMed Central - PubMed

Affiliation: aDepartment of Oncology, Medicine I bCenter for Physiology and Pharmacology, Comprehensive Cancer Center, Institute of Pharmacology cDepartment of Neurology, Medical University of Vienna, Vienna, Austria.

No MeSH data available.


Related in: MedlinePlus