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The Tinkerbell (Tink) Mutation Identifies the Dual-Specificity MAPK Phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) as a Novel Regulator of Organ Size in Arabidopsis.

Johnson KL, Ramm S, Kappel C, Ward S, Leyser O, Sakamoto T, Kurata T, Bevan MW, Lenhard M - PLoS ONE (2015)

Bottom Line: Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway.Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development.We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.

View Article: PubMed Central - PubMed

Affiliation: ARC Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Royal Parade, Parkville, Victoria, 3010, Australia.

ABSTRACT
Mitogen-activated dual-specificity MAPK phosphatases are important negative regulators in the MAPK signalling pathways responsible for many essential processes in plants. In a screen for mutants with reduced organ size we have identified a mutation in the active site of the dual-specificity MAPK phosphatase indole-3-butyric acid-response5 (IBR5) that we named tinkerbell (tink) due to its small size. Analysis of the tink mutant indicates that IBR5 acts as a novel regulator of organ size that changes the rate of growth in petals and leaves. Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway. Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development. We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.

No MeSH data available.


Related in: MedlinePlus

Double mutant analysis of tink/ibr5-6 klu-2 mutants compared to single mutants and wild-type.a. A significant reduction in petal area (p value ≤ 2.4e-7) is seen in klu-2 mutants compared to wild-type and the same relative decrease in petal size occurs in double mutants of tink/ibr5-6 with klu-2. b. Cell size in klu-2 mutants is significantly reduced (p value ≤ 0.02) compared to Ler and the same relative decrease in cell size occurs in tink/ibr5-6 klu-2 mutants. Values are shown as mean ± SEM, with n = 10.
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pone.0131103.g004: Double mutant analysis of tink/ibr5-6 klu-2 mutants compared to single mutants and wild-type.a. A significant reduction in petal area (p value ≤ 2.4e-7) is seen in klu-2 mutants compared to wild-type and the same relative decrease in petal size occurs in double mutants of tink/ibr5-6 with klu-2. b. Cell size in klu-2 mutants is significantly reduced (p value ≤ 0.02) compared to Ler and the same relative decrease in cell size occurs in tink/ibr5-6 klu-2 mutants. Values are shown as mean ± SEM, with n = 10.

Mentions: The tink/ibr5-6 mutant was identified in a mutagenesis screen as an enhancer of the klu-2 mutant phenotype. The cytochrome P450 KLUH (KLU)/CYP78A5 is presumed to generate a growth-promoting signal that acts in a regulatory mechanism to coordinate the growth of individual organs [25]. Increased activity of KLU causes organ overgrowth, while klu mutants form smaller aerial organs consisting of fewer cells. Detailed investigation of double mutant tink/ibr5-6 klu-2 plants shows an additive effect to decrease petal size (Fig 4). This suggests that IBR5 acts independently of KLU regulatory pathways.


The Tinkerbell (Tink) Mutation Identifies the Dual-Specificity MAPK Phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) as a Novel Regulator of Organ Size in Arabidopsis.

Johnson KL, Ramm S, Kappel C, Ward S, Leyser O, Sakamoto T, Kurata T, Bevan MW, Lenhard M - PLoS ONE (2015)

Double mutant analysis of tink/ibr5-6 klu-2 mutants compared to single mutants and wild-type.a. A significant reduction in petal area (p value ≤ 2.4e-7) is seen in klu-2 mutants compared to wild-type and the same relative decrease in petal size occurs in double mutants of tink/ibr5-6 with klu-2. b. Cell size in klu-2 mutants is significantly reduced (p value ≤ 0.02) compared to Ler and the same relative decrease in cell size occurs in tink/ibr5-6 klu-2 mutants. Values are shown as mean ± SEM, with n = 10.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492785&req=5

pone.0131103.g004: Double mutant analysis of tink/ibr5-6 klu-2 mutants compared to single mutants and wild-type.a. A significant reduction in petal area (p value ≤ 2.4e-7) is seen in klu-2 mutants compared to wild-type and the same relative decrease in petal size occurs in double mutants of tink/ibr5-6 with klu-2. b. Cell size in klu-2 mutants is significantly reduced (p value ≤ 0.02) compared to Ler and the same relative decrease in cell size occurs in tink/ibr5-6 klu-2 mutants. Values are shown as mean ± SEM, with n = 10.
Mentions: The tink/ibr5-6 mutant was identified in a mutagenesis screen as an enhancer of the klu-2 mutant phenotype. The cytochrome P450 KLUH (KLU)/CYP78A5 is presumed to generate a growth-promoting signal that acts in a regulatory mechanism to coordinate the growth of individual organs [25]. Increased activity of KLU causes organ overgrowth, while klu mutants form smaller aerial organs consisting of fewer cells. Detailed investigation of double mutant tink/ibr5-6 klu-2 plants shows an additive effect to decrease petal size (Fig 4). This suggests that IBR5 acts independently of KLU regulatory pathways.

Bottom Line: Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway.Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development.We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.

View Article: PubMed Central - PubMed

Affiliation: ARC Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Royal Parade, Parkville, Victoria, 3010, Australia.

ABSTRACT
Mitogen-activated dual-specificity MAPK phosphatases are important negative regulators in the MAPK signalling pathways responsible for many essential processes in plants. In a screen for mutants with reduced organ size we have identified a mutation in the active site of the dual-specificity MAPK phosphatase indole-3-butyric acid-response5 (IBR5) that we named tinkerbell (tink) due to its small size. Analysis of the tink mutant indicates that IBR5 acts as a novel regulator of organ size that changes the rate of growth in petals and leaves. Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway. Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development. We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.

No MeSH data available.


Related in: MedlinePlus