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Assessment of the Impact of Zoledronic Acid on Ovariectomized Osteoporosis Model Using Micro-CT Scanning.

Shuai B, Shen L, Yang Y, Ma C, Zhu R, Xu X - PLoS ONE (2015)

Bottom Line: The impact of ovariectomy, the effect of estradiol, and the effect of zoledronic acid intervention on cancellous bone microstructure, as well as on the expression of β-catenin, were evaluated.The estradiol-treated and the zoledronic acid-treated group exhibited a significant increase in the bone volume fraction, trabecular number, trabecular thickness, bone surface to bone volume ratio (BS/BV), and β-catenin expression, when compared with those of the control group (P <0.01).In contrast, the structure model index, trabecular separation, and BS/BV were significantly lower compared with those of the model group (P <0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

ABSTRACT

Purpose/objective: Prompted by preliminary findings, this study was conducted to investigate the impact of zoledronic acid on the cancellous bone microstructure and its effect on the level of β-catenin in a mouse model of postmenopausal osteoporosis.

Methods and materials: 96 8-week-old specific-pathogen-free C57BL/6 mice were randomly divided into 4 groups (24 per group): a sham group, an ovariectomized osteoporosis model group, an estradiol-treated group, and a zoledronic acid-treated group. Five months after surgery, the third lumbar vertebra and left femur of the animals were dissected and scanned using micro-computed tomography (micro-CT) to acquire three-dimensional imagery of their cancellous bone microstructure. The impact of ovariectomy, the effect of estradiol, and the effect of zoledronic acid intervention on cancellous bone microstructure, as well as on the expression of β-catenin, were evaluated.

Results: The estradiol-treated and the zoledronic acid-treated group exhibited a significant increase in the bone volume fraction, trabecular number, trabecular thickness, bone surface to bone volume ratio (BS/BV), and β-catenin expression, when compared with those of the control group (P <0.01). In contrast, the structure model index, trabecular separation, and BS/BV were significantly lower compared with those of the model group (P <0.01). No differences were observed in the above parameters between animals of the zoledronic acid-treated and the estradiol-treated group.

Conclusion: These results suggest that increased β-catenin expression may be the mechanism underlying zoledronic acid-related improvement in the cancellous bone microstructure in ovariectomized mice. Our findings provide a scientific rationale for using zoledronic acid as a therapeutic intervention to prevent bone loss in post-menopausal women.

No MeSH data available.


Related in: MedlinePlus

Micro-CT of left femurs in the four groups.(2a-2d) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of distal femoral metaphysis (cross section) in four groups. (2e-2h) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of whole left femur (longitudinal section) in four groups.
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pone.0132104.g002: Micro-CT of left femurs in the four groups.(2a-2d) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of distal femoral metaphysis (cross section) in four groups. (2e-2h) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of whole left femur (longitudinal section) in four groups.

Mentions: The parameters of the trabecular bone after 3-D reconstruction demonstrated that, in animals of the ovariectomized osteoporosis model group, the bone density was significantly attenuated, the bone micro-architecture severely impaired, the trabeculae were thin and less dense, and the connectivity was reduced, when compared with the sham groups. The rod-like trabeculae outnumbered plate-like structures, and larger inter-trabecular spaces were present in animals of the ovariectomized osteoporosis model group (Figs 2 and 3). Furthermore, their BV/TV, Tb.N., and Tb.Th. were significantly lower and their SMI, Tb.Sp., and BS/BV were significantly higher compared with the animals in the sham group (both P <0.01), indicating the successful establishment of the experiment model (Figs 4 and 5).


Assessment of the Impact of Zoledronic Acid on Ovariectomized Osteoporosis Model Using Micro-CT Scanning.

Shuai B, Shen L, Yang Y, Ma C, Zhu R, Xu X - PLoS ONE (2015)

Micro-CT of left femurs in the four groups.(2a-2d) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of distal femoral metaphysis (cross section) in four groups. (2e-2h) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of whole left femur (longitudinal section) in four groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492783&req=5

pone.0132104.g002: Micro-CT of left femurs in the four groups.(2a-2d) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of distal femoral metaphysis (cross section) in four groups. (2e-2h) Three-dimensional images reconstructed from micro-CT analysis on the cortical and trabecular bone microarchitecture of whole left femur (longitudinal section) in four groups.
Mentions: The parameters of the trabecular bone after 3-D reconstruction demonstrated that, in animals of the ovariectomized osteoporosis model group, the bone density was significantly attenuated, the bone micro-architecture severely impaired, the trabeculae were thin and less dense, and the connectivity was reduced, when compared with the sham groups. The rod-like trabeculae outnumbered plate-like structures, and larger inter-trabecular spaces were present in animals of the ovariectomized osteoporosis model group (Figs 2 and 3). Furthermore, their BV/TV, Tb.N., and Tb.Th. were significantly lower and their SMI, Tb.Sp., and BS/BV were significantly higher compared with the animals in the sham group (both P <0.01), indicating the successful establishment of the experiment model (Figs 4 and 5).

Bottom Line: The impact of ovariectomy, the effect of estradiol, and the effect of zoledronic acid intervention on cancellous bone microstructure, as well as on the expression of β-catenin, were evaluated.The estradiol-treated and the zoledronic acid-treated group exhibited a significant increase in the bone volume fraction, trabecular number, trabecular thickness, bone surface to bone volume ratio (BS/BV), and β-catenin expression, when compared with those of the control group (P <0.01).In contrast, the structure model index, trabecular separation, and BS/BV were significantly lower compared with those of the model group (P <0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

ABSTRACT

Purpose/objective: Prompted by preliminary findings, this study was conducted to investigate the impact of zoledronic acid on the cancellous bone microstructure and its effect on the level of β-catenin in a mouse model of postmenopausal osteoporosis.

Methods and materials: 96 8-week-old specific-pathogen-free C57BL/6 mice were randomly divided into 4 groups (24 per group): a sham group, an ovariectomized osteoporosis model group, an estradiol-treated group, and a zoledronic acid-treated group. Five months after surgery, the third lumbar vertebra and left femur of the animals were dissected and scanned using micro-computed tomography (micro-CT) to acquire three-dimensional imagery of their cancellous bone microstructure. The impact of ovariectomy, the effect of estradiol, and the effect of zoledronic acid intervention on cancellous bone microstructure, as well as on the expression of β-catenin, were evaluated.

Results: The estradiol-treated and the zoledronic acid-treated group exhibited a significant increase in the bone volume fraction, trabecular number, trabecular thickness, bone surface to bone volume ratio (BS/BV), and β-catenin expression, when compared with those of the control group (P <0.01). In contrast, the structure model index, trabecular separation, and BS/BV were significantly lower compared with those of the model group (P <0.01). No differences were observed in the above parameters between animals of the zoledronic acid-treated and the estradiol-treated group.

Conclusion: These results suggest that increased β-catenin expression may be the mechanism underlying zoledronic acid-related improvement in the cancellous bone microstructure in ovariectomized mice. Our findings provide a scientific rationale for using zoledronic acid as a therapeutic intervention to prevent bone loss in post-menopausal women.

No MeSH data available.


Related in: MedlinePlus